Browsing by Author "Ortega Puma, Carmen Maribel"

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Efecto antidepresivo del tallo de trichocereus pachonoi
(2009) Bermejo, Fernanda; Ortega Puma, Carmen Maribel; Zaruma Torres, Fausto Leonardo
Evaluación de las variables altitud y composición del suelo sobre la actividad antibacteriana y antifúngica de extractos lipofílicos de hypericum laricifolium juss por fingerprint cromatográfico
(2017-09-01) Ortega Puma, Carmen Maribel; León Tamariz, Fabián; Santos Ordoñez, Efrén Germán; Ortiz Ulloa, Silvia Johana
Hypericum laricifolium Juss is used for the treatment of microbial infections. The secondary metabolic profile and hence the biological activity could be influenced by several environmental variables. The aim of this work was to evaluate the effect of altitude and soil compositions on the antibacterial and antifungal activity of lipophilic extracts of Hypericum laricifolium, by evaluating 29 individuals and their soil samples obtained in different environmental conditions and to get a predictive model of their effect using fingerprint chromatography by HPLC. Soil samples were analyzed for pH, electrical conductivity, organic matter, nitrogen, phosphorus and potassium. The extracts were obtained by supercritical fluid extraction. For molecular identification, the loci ITS1, ITS2 and rbcL were sequenced. The identity for ITS1 and ITS2 was 100% and 99.7% respectively, rbcL exhibited genus identity (98,5%). The antibacterial activity was performed by micro-dilution technique against MRSA. A high activity has been detected. Using partial least squares (PLS) a correlation was established between antibacterial activity and chromatographic profiles at 254, 280 and 360 nm. The model was constructed using the individuals with their replicas, with 7501 X variables (responses at different retention times), variable y (IC50 of each individual). The model was evaluated using R2 correlation parameters, the model at 254 nm predicted better the antibacterial activity (R2=0.92).
Evaluation of the variables altitude, soil composition and development of a predictive model of the antibacterial activity for the genus Hypericum by chromatographic fingerprint
(2019) Ortega Puma, Carmen Maribel; Fajardo Carmona, Sandra Isabel; Ortiz Ulloa, Silvia Johana; Tobar Solano, Vladimiro Alexis; Quito Ávila, Diego; Santos Ordóñez, Efrén; Jerves Andrade, María de Lourdes; Cuzco Quizhpi, Nancy Mirian; Wilches Arizabala, Isabel Maria; León Tamariz, Fabián
H. laricifolium Juss is a native shrub from the Andes used for the treatment of antibacterial infections among other illnesses. Nowadays, due to the growing need of new antibiotic substances, this medicinal plant would represent an important source for the investigation of antibacterial activity due to the wide medicinal potential of its genus, property that can be analyzed through its chemical fingerprint profile. The objectives of this research were, to develop a predictive model for the antibacterial potential of the genus Hypericum using chromatographic fingerprinting with three Hypericum species collected at Ecuadorian Andes (H. laricifolium, H. quitense R. Keller and H. decandrum Turcz); and to evaluate the influence of natural variables such as altitude and soil composition on the pharmacological effect of lipophilic extracts of H. laricifolium. Antibacterial activity of the plants extracts was assessed by plate microdilution technique against Methicillin-resistant Staphylococcus aureus (MRSA). The fingerprint was obtained by HPLC-DAD. The predictive model was constructed from the chromatographic fingerprint of H. laricifolium and H. quitense and their IC50 values using the “Partial least squares” multivariate analysis tool. Using the regression coefficients of the model, the predictive equation of antibacterial activity for new samples of H. decandrum was established. The extracts of H. laricifolium exhibited a high antibacterial activity with the pH and the organic matter as main factors influencing the activity. The prediction model obtained did not have predictive ability to different Hypericum species, which could be explained by differences in the chemical composition of the three species.