Browsing by Author "Mendez Ortiz, Yessenia Estefania"

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    Evaluación del método PCR para la detección de material genético de Brucella spp en órganos reproductivos y sangre de perros seropositivos a inmunocromatografía
    (Universidad de Cuenca. Facultad de Ciencias Agropecuarias, 2026-02-12) Buñay Guaman, Jennyfer Teresa; Mendez Ortiz, Yessenia Estefania; Picón Saavedra, Marco Antonio
    Canine brucellosis is an infectious disease caused by bacteria of the genus Brucella, mainly the species B. canis, which is of great importance due to its zoonotic nature in public health. Effective and reliable diagnosis is essential for controlling the disease and preventing its transmission in both animals and humans. There are various diagnostic techniques for the detection of Brucella spp., among the most important of which are serological and molecular tests; however, polymerase chain reaction (PCR) is considered one of the most specific and sensitive for the identification of this bacterium. The objective of this study was to evaluate the detection of Brucella spp. using the PCR technique in blood and reproductive organ samples (uterus, testicles, and epididymis) from dogs seropositive for Brucella using the immunochromatography test. A total of 76 samples were analyzed, corresponding to 38 blood samples and 38 tissue samples. The results obtained showed four positive samples by PCR, of which two corresponded to blood and two to tissue. This represents 5,3% of the total number of seropositive dogs. Based on the results obtained, it is concluded that both blood and reproductive organ tissue samples are useful for the diagnosis of Brucella spp. by PCR. However, the accuracy of the results depends largely on the proper handling of samples and the application of laboratory protocols in order to avoid erroneous results or incorrect interpretations.