Browsing by Author "Malla Bravo, Ana Cristina"

Now showing 1 - 2 of 2

Determinación del metabolito tóxico aflatoxina M1 en leches cruda, pasteurizada y ultrapasteurizada consumidas en la ciudad de Cuenca mediante la técnica de cromatografía líquida de alta resolución (HPLC)
(2016) Malla Bravo, Ana Cristina; Saula López, Sandra Vanessa; Uguña Rosas, María Fernanda; Ortiz Ulloa, Silvia Johana
Aflatoxins are very powerful and immunosuppressive toxic metabolites carcinogenic, teratogenic, mutagenic, hepatotoxic, which constitute a potential health risk to humans, so it should be considered as a potential problem that needs to be continually reviewed in order to ensure the quality of food. This study was conducted to quantitatively determine the content of aflatoxin M1 in three types of raw milk, pasteurized and ultra-pasteurized consumed in the city of Cuenca, by the technique of high performance liquid chromatography HPLC resolution, following an extraction process immunoaffinity columns. Analyzing 84 samples in total, it was made for each kind of milk two corresponding samples each in different production batches, three samples per lot, ie six samples by mark in the case of whole and skimmed milk were carried out UHT and two samples in the case of whole milk pasteurized and raw milk. , Giving a prevalence of 19% - 16 positive samples that were between the detection limit and quantification (0.18 ppb 0.09) were found. AFM1 values were lower than that permitted by current national regulations INEN 9-10 (0.5 ppb) and internationally by the FDA (0.5 ppb) concentration; therefore, the concentration of AFM1 in milk analyzed by the technique of high performance liquid chromatography HPLC resolution does not contain levels that constitute a danger to the health of the consumer population.
Patologías causantes del decomiso de vísceras y canales bovinas según edad, sexo y raza en el centro de beneficio de Paute
(Universidad de Cuenca, 2023-03-27) Centeno Centeno, Libia Liliana; Malla Bravo, Ana Cristina; López Crespo, Gonzalo Estuardo
Pathologies causing confiscation of channels and viscera of slaughtered bovines cause public health problems and economic losses; Above all, not having studies that relate these alterations with variables such as "age, race and sex." This work was carried out in order to establish the type of injury that causes the confiscation and determine its percentage in relation to the three variables mentioned; In addition, an atlas was developed from the findings of this investigation. The main alterations found correspond to pathologies of the cardiac, respiratory, genitourinary, digestive and trauma system. The study was executed at the benefit center of the Paute canton located in the province of Azuay. A post mortem observation was performed for the identification and classification of pathologies. A chisquare test was carried out to determine the association of the frequency of the lesions with the variables of the study. The results showed that in relation to the sex variable, the females presented injuries more frequently. According to age, bovines 49 months and older (discard) presented more injuries than bovines of 4-23 months (young) and those of 24-48 months (reproduction). The races that presented a higher percentage of injuries are Creole, Holstein and Brown Swiss. Relationship of the lesions with the age and sex variables were found. In conclusion, the main pathologies causing confiscation were distomatosis, multifocal hemorrhages in lung, liver abscesses and interstitial emphysema.