Browsing by Author "Machuca Machuca, Alejandro Armando"

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    Efecto de la L-carnitina y la melatonina sobre la calidad espermática de gallo criollo (gallus gallus domesticus) previamente congelado con dimetilformamida
    (Universidad de Cuenca, 2024-07-16) Machuca Machuca, Alejandro Armando; Galarza Lucero, Diego Andrés
    Cold shock and osmotic stress are two factors that determine the cryosurvival of rooster sperm. To mitigate these effects, the supplementation of antioxidant additives to freezing media has been suggested. This research evaluated the effect of two antioxidant- additives, melatonin (MEL) and L-carnitine (LC), onkinematics and plasma membrane integrity (viability) of Creole rooster sperm (Gallus gallus domesticus) previously frozen with dimethylformamide. A group without additive was used as a control. The straws (0.25 ml) from the MEL (n = 100), LC (n = 100) and control (n = 100) groups were thawed by immersing them in cold water (5°C) for 3 minutes. Post-thawing, kinematics and viability were objectively evaluated using the CASA computerized system (SCA- Evolution 2018) and the propidium iodide fluorescent test, respectively. A one-way ANOVA and Tukey's multiple comparison test were used to evaluate the effect of the three treatments. The results demonstrated that sperm samples previously frozen with LC produced higher values (P < 0.05) of viability, progressive motility, curvilinear, rectilinear and average speed, and flagellum beating frequency compared to those sperm samples previously frozen with MEL. And control. On the other hand, samples frozen with MEL, although its effect was limited on viability, produced higher values (P < 0.05) of curvilinear velocity and amplitude of lateral head displacement compared to samples from the control group. In conclusion, LC supplementation to the freezing medium produced better sperm cryosurvival based on greater membrane integrity and improved kinematics