Browsing by Author "Lucero Zhumi, Juan Ismael"

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Efecto de la jalea real suplementada a diluyentes de base sintética y no sintética sobre criosupervivencia de esperma bovino
(Universidad de Cuenca, 2022-11-22) Guanga Tenempaguay, Jacqueline Estefanía; Lucero Zhumi, Juan Ismael; Galarza Álvarez, Luis Rodrigo
Royal jelly (RJ) is a nutrient produced by nurse bees (4 and 11 days old) and contains vitamins, proteins, enzymes, amino acids, and other minerals. There is limited information on the positive effect of royal jelly in ruminant sperm cryopreservation; its beneficial components may be enhanced in different diluents. This investigation evaluated the effect of RJ supplementation in synthetic (TCG= tris, acid, citric, glucose + 6% egg yolk) and non-synthetic (UHT= skim milk + 6% egg yolk) diluents on cryosurvival of bull spermatozoa after refrigeration, freezing and vitrification processes. In a first experiment, 9 ejaculates from 3 healthy bulls (3 ejaculates/week) were used to determine the most suitable concentration of RJ (w/v) at 0% (T0, control); 0,2% (T1); 0,4% (T2); 0,6% (T3); 0,8% (T4) and 1% (T5) supplemented with TCG and UHT extenders on the kinematics of refrigerated sperm for 96 hours. The results demonstrated that a low dose of 0,2% RJ (T1) supplemented to both extenders has a positive effect on progressive motility and velocities (curvilinear [VCL] and rectilinear [VSL]) of chilled bull sperm in the longterm. In a second experiment, 15 ejaculates from 3 bulls (3 ejaculates/week) were used to evaluate the effect of supplementation of 0,2% RJ (w/v) to freezing extenders (TCG+5% glycerol) and vitrification (TCG+100 sucrose). Four groups were formed according to the cryopreservation method (freezing [CONG] and vitrification [VIT]) and the addition or not (control) of RJ: (1) CONG-Co, (2) CONG-RJ, (3) VIT-Co, (4) VIT-RJ. The results showed that during freezing (CONG-RJ vs. CONG-Co, P<0.05), RJ increased VCL, VSL, and beat frequency (BCF), while during vitrification (VIT-RJ vs. VIT-Co, P<0,05), the RJ improved the BCF and integrity of plasma and acrosome membranes. It is concluded that the addition of 0,2% RJ to TCG and UHT extenders improved chilled bovine sperm kinematics. In addition, The RJ better protects sperm membranes during vitrification than the freezing process.
La suplementación de jalea real a diluyente de base sintética y no sintética ayuda a mantener la motilidad y cinética de esperma de toro refrigerado
(Universidad de Cuenca, 2022) Galarza Lucero, Diego Andrés; Guanga Tenempaguay, Jacqueline; Lucero Zhumi, Juan Ismael; Samaniego Campoverde, Jorge Xavier; Galarza Lucero, Diego Andrés; Galarza Lucero, Diego Andrés
Royal Jelly (RJ) is a substantial nutrient produced by the hypopharynx and mandibular glands of worker bees from 4 to 11 days of age, and its composition is mainly based on water (60–70%), protein (27–41% ) and carbohydrates (30%), lipids (8–19%), vitamins (A, B, C, E), salts, and amino acids. Due to its composition, JR has been successfully used in sperm cryopreservation at concentrations from 0.2 to 1% (w/v) supplemented with synthetic base extenders (eg TRIS or TALP). Its effect has been evidenced in the increase of motility, viability, functionality of the plasmatic membrane and fertilizing capacity of fresh and cryopreserved ram sperm. Conventional freezing requires the use of penetrating cryoprotectants (eg glycerol) to prevent damage caused by ice crystal formation, however, vitrification requires high concentrations (0.1 to 6 M) of non-penetrating cryoprotectants (eg sucrose or trehalose) and rapid cooling, passing rapidly to the glass transition without forming ice crystals. This research evaluated the effect of JR (0.2%) supplemented to freezing (TCG-YH + 5% glycerol) and vitrification (TCG-YH + 100 mM sucrose) media on cryosurvival of bull sperm.