Browsing by Author "Lema Segovia, Geovanna Daniela"

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    Evaluación del antígeno ESAT6-CFP10 recombinante de Mycobacterium tuberculosis para la detección de anticuerpos en primates no humanos potencialmente expuestos a tuberculosis
    (Universidad de Cuenca, 2025-09-03) Lema Segovia, Geovanna Daniela; Vele Criollo, Daniela Belén; Vallecillo Maza, Antonio Javier
    Mycobacterium tuberculosis complex is made up of bacterial species belonging to the Mycobacteriacea family, most of which cause tuberculosis, a zoonotic disease considered of public health importance. These bacterial pathogens are considered Gram-positive, non-sporulating, and capable of intracellular residence. In non-human primates, the species M. tuberculosis, M. bovis, and additionally M. avium have been identified. The main infection occurs through the inhalation of aerosols expelled from the respiratory tract of both animals and humans who have been infected with the active disease. Since these are wildlife, their current status in these species is unknown. The present study aimed to evaluate ESAT-6 and CFP-10 antigens integrated into a fusipon protein, for the detection of anti-M. tuberculosis in Capuchino monkeys (Cebus capucinus) and Chorongo (Lagothrix poeppigii) potentially exposed to this disease, for which a total of 14 blood samples were collected (10 from Capuchin monkeys and 4 from Chorongo) residing in a zoo-shelter in the city of Cuenca, these samples were analyzed using Western blot and indirect ELISA diagnostic techniques using the recombinant antigen HT-ESAT6-CFP10. The results obtained show that, of the 14 analyzed sera, no reactivity to the M. tuberculosis HT-ESAT6-CFP10 antigens was evident in the Western blot assay. However, the in-house indirect ELISA showed signs of reactivity, which can be assumed to be suspected cases. Finally, no differences were found in the application of either of the two diagnostic methods for the identification of tuberculosis in non-human primates.