Browsing by Author "Lema Fajardo, Wilson Mauricio"

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    Producción de las proteínas bovinas CLRN3 y SCAMP1 en un modelo de expresión bacteriano basado en Escherichia coli
    (Universidad de Cuenca. Facultad de Ciencias Agropecuarias, 2026-02-12) Lema Fajardo, Wilson Mauricio; Vallecillo Maza, Antonio Javier
    The membrane proteins BovCLRN3 and BovSCAMP1, differentially expressed in bovine “X” and “Y” spermatozoa, respectively, have been recently identified. Despite the limited information available regarding their functionality, both proteins have been proposed as specific antigenic candidates for immunological separation. The production of these proteins in a heterologous system would strengthen this possibility; however, no reports to date describe their recombinant expression in such systems. Based on this background, the present study focused on producing the recombinant proteins BovCLRN3 and BovSCAMP1 from cattle (Bos taurus) for their subsequent evaluation in the development of sperm‑sexing methods as alternatives to flow cytometry. As a result, the recombinant BovSCAMP1 protein was successfully produced using a bacterial expression model with the Escherichia coli Rosetta 2 (DE3) strain, establishing a reproducible protocol for its production. In contrast, the production of BovCLRN3 could not be achieved, a result that may be associated with technical limitations related to its expression, suggesting the need to optimize expression systems or culture conditions in future research.