Browsing by Author "Intriago Morales, Ruth Viviana"

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Adipokines in dental pulp: physiological, pathological and potential therapeutic roles
(Universidad de Cuenca, 2022-02-11) Gavidia Pazmiño, Jonathan Francisco; Bravo Guapisaca, María Isabel; Intriago Morales, Ruth Viviana
Background: Hundreds of adipokines have been identified, and their extensive range of endocrine functions—regulating distant organs such as oral tissues—and local autocrine/paracrine roles have been studied. In dentistry, however, adipokines are poorly known proteins in the dental pulp; few of them have been studied despite their large number. This study reviews recent advances in the investigation of dental-pulp adipokines, with an emphasis on their roles in inflammatory processes and their potential therapeutic applications. Highlights: The most recently identified adipokines in dental pulp include leptin, adiponectin, resistin, ghrelin, oncostatin, chemerin, and visfatin. They have numerous physiological and pathological functions in the pulp tissue: they are closely related to pulp inflammatory mechanisms and actively participate in cell differentiation, mineralization, angiogenesis, and immune-system modulation. Conclusion: Adipokines have potential clinical applications in regenerative endodontics and as biomarkers or targets for the pharmacological management of inflammatory and degenerative processes in dental pulp. A promising direction for the development of new therapies may be the use of agonists/antagonists to modulate the expression of the most studied adipokines.
Adipokines in dental pulp: physiological, pathological, and potential therapeutic roles
(2021) Gavidia Pazmiño, Jonathan Francisco; Intriago Morales, Ruth Viviana; Bravo Guapisaca, Maria Isabel; Álvarez Vásquez, José Luis
Background: Hundreds of adipokines have been identified, and their extensive range of endocrine functions- regulating distant organs such as oral tissues- and local autocrine/paracrine roles have been studied. In dentistry, however, adipokines are poorly known proteins in the dental pulp; few of them have been studied despite their large number. This study reviews recent advances in the investigation of dental-pulp adipokines, with an emphasis on their roles in inflammatory processes and their potential therapeutic applications. Highlights: The most recently identified adipokines in dental pulp include leptin, adiponectin, resistin, ghrelin, oncostatin, chemerin, and visfatin. They have numerous physiological and pathological functions in the pulp tissue: they are closely related to pulp inflammatory mechanisms and actively participate in cell differentiation, mineralization, angiogenesis, and immune-system modulation. Conclusion: Adipokines have potential clinical applications in regenerative endodontics and as biomarkers or targets for the pharmacological management of inflammatory and degenerative processes in dental pulp. A promising direction for the development of new therapies may be the use of agonists/antagonists to modulate the expression of the most studied adipokines.
Comparación de tres técnicas de irrigación en la remoción de hidróxido de calcio
(2018) Intriago Morales, Ruth Viviana; Ortiz Garay, Elida Magaly; Narvaez Miranda, Deborath Estefania; Vega Yepez, Andres Gustavo; Villavicencio Caparó, Ebingen
Objectives: To compare efﬁ cacy of three irrigation protocols in the removal of calcium hydroxide [Ca(OH)2]. Materials and Methods: Through an in vitro comparative study design, 106 root canals of bovine incisors were instrumented up to a ﬁ le of diameter 60, the roots were fractioned into two halves following the major axis of the tooth and a standardized groove was created 2mm from the apical foramen that was ﬁ lled with a paste of calcium hydroxide, then the halves were reassembled, incubated for 7 days and the irrigation protocols were carried out: passive ultrasonic (PUI), sonic with Endo Activator (EA) and manual dynamic activation (MDA), where 5% sodium hypochlorite (NaOCl) was used as irrigant. A negative and positive control was used. With a stereomicroscope the amount of residue was observed, the images were examined and assigned a score according to the van der Sluis scale, ﬁ nally the data were analyzed with the statistical test of Chi-square. Results: The percentages of efﬁ cacy for PUI, EA and MDA irrigation protocols were 87.5%; 46.9% and 28.1% respectively, the PUI technique was superior to EA and MDA and obtained statistically signiﬁ cant differences (p <0.001). Conclusions: PUI was the most effective method in the removal of Ca(OH)2 from the simulated grooves in the root canals.
Comparación del sellado del conducto radicular con la técnica de obturación de condensación lateral y cono único, usando cemento biocerámico y a base de resina epóxica
(Universidad de Cuenca, 2025-01-13) Saldaña Ayala, María Gabriela; Intriago Morales, Ruth Viviana
Root canal obturation is crucial for the success of endodontic treatment. This study compares the lateral condensation technique with the single cone technique, using bioceramic cement (BioRoot Flow) and epoxy-based cement (AH Plus Sealer). The experimental in vitro design involved 72 bovine teeth, divided into four groups: G1, single cone technique with bioceramic cement; G2, single cone with epoxy cement; G3, lateral condensation with epoxy cement; and G4, lateral condensation with bioceramic cement. The teeth were cleaned and standardized to 18 mm. After chemomechanical preparation of the canals, obturation was performed using the selected technique and cement, sealed with glass ionomer cement, and incubated in saline solution at 37°C for 48 hours. The roots were then submerged in 1% methylene blue for 72 hours to assess apical microleakage, followed by a longitudinal sectioning that allowed observation under a stereomicroscope and measurement of dye penetration, categorized based on the depth of leakage. The results showed that G1 exhibited the least microleakage (1-3 mm), while G4 performed the worst (≥ 5 mm). Significant differences were found between G1 and G2 (p = 0.037), as well as between G1 and G4 (p = 0.001). G2 and G3, which used epoxy-based cement, did not show significant differences between them. In conclusion, the single cone technique with BioRoot Flow bioceramic cement demonstrated superior performance.
Eficacia de la irrigación ultrasónica, sónica y agitación manual dinámica en la remoción de hidróxido de calcio de conductos radiculares de dientes Bovinos
(2017) Intriago Morales, Ruth Viviana; Vega Yépez, Andrés Gustavo
Objective: To compare the irrigation techniques: ultrasonic, sonic and manual dynamic activation in the removal of Ca(OH)2 from simulated grooves in bovine teeth. Materials and Methods: An in vitro study was performed on one hundred and six bovine incisors, where their root canals were prepared up to a file of diameter 60. The roots were divided longitudinally to create a groove 2mm from the apex which was filled with Ca(OH)2, then the halves were reassembled and after 7 days of incubation the techniques were applied: passive ultrasonic irrigation (PUI), sonic irrigation [Endo Activator (EA)] and manual dynamic activation (MDA), using 5% NaOCl as irrigant. Positive control and negative control were used. Samples were analyzed using a stereomicroscope and images were taken with a digital camera. Two calibrated observers (Kappa> 0.8) evaluated the removal according to the van der Sluis scores and the results were analyzed using the Chi Square test. Results: Effectiveness percentages in Ca(OH)2 removal were 87.5%; 46.9% and 28.1% for PUI, EA and MDA respectively. The PUI irrigation protocol was superior and obtained a statistically significant difference compared to the other evaluated techniques (P <0.01). Conclusions: PUI eliminated significantly more Ca(OH)2 from the simulated grooves than the EA and MDA protocols. Keywords: Calcium hydroxide removal, ultrasonic irrigation, sonic irrigation, Endo Activator, manual dynamic activation, endodontics
Elaboración de un biodiente: enfoque actual y desafíos
(2018) Intriago Morales, Ruth Viviana; Álvarez Vásquez, José Luis
Background: Edentulism is one of the major oral health problems that cause physiological, social,aesthetic, phonetic, and nutritional issues. Current therapies for dental replacement are artificial and do not satisfy the basic requirements of a natural tooth. Tissue bioengineering could be a viable alternative to substitute lost teeth. Objective: To identify current available approaches/techniques to obtain a complete bioengineered tooth (bio-tooth) and to point out future challenges and perspectives. Methods: This was an integrative literature review. Search keywords used were: bio-tooth, tissue bioengineering, whole tooth, stem cells. The search included the years 2000 through 2018, using the databases PubMed, Scopus, EBSCO, Science Direct, Wiley Online Library, Lilacs and Google Scholar, both in English and Spanish. Only relevant and pertinent articles and books were selected. Results: 53 articles and 10 books were obtained. Methods for bio-tooth generation found were: scaffolds, scaffold-free, induced pluripotent stem cells, tooth organ germ, chimeric tooth, and stimulation of third dentition formation. Achieving normal tooth size and shape and obtaining epithelial cells are the main challenges. Conclusions: The possibility of creating and developing a whole bioengineered tooth (bio-tooth) in an adult oral environment is becoming more realistic, considering the daily biotechnological advances. It is possible that these concepts will be the basis of restorative dentistry in a near future.
Inómeros de vidrio
(2010) Intriago Morales, Ruth Viviana; Abad Coronel, Dunia Elizabeth