Browsing by Author "Guanga Lucero, Evelyn Daniela"

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    Efecto de la exposición a la luz artificial sobre calidad espermática, morfometría testicular y niveles de testosterona en cuyes
    (Universidad de Cuenca, 2024-03-18) Guanga Lucero, Evelyn Daniela; Quilli Barbecho, María Beatriz; Rosales Jaramillo, Cornelio Alejandro
    The study evaluated exposure to artificial light on sperm quality, testicular morphometry and testosterone levels in guinea pigs. Forty-eight male guinea pigs were divided into four randomized treatments: T1: 24H light, T2: 18H light, T3: 12H light and T4: 0H no exposure. Blood testosterone was evaluated on days 1, 30, 60 of the experiment. Testicular morphometry was determined after castration; for the assessment of sperm quality, epididymal spermatozoa were recovered by retrograde flow using 0.5 ml of commercial diluent (Botu- Semen special®). The results showed that artificial light significantly (p<0.05) affected left (ETI) and right (ETD) testis thickness at T1. Testosterone levels at day 60 were significantly higher at T3 (5.22 ng/ml) (p<0.05) compared to T2 (2.76 ng/ml), with no difference between T1 and T4. On sperm quality, significant differences (p<0.05) were found in sperm concentration between T4 and T3, but did not differ in the other treatments; for kinetic parameters total motility (MT) was higher (p<0.05) in T4 (81.33%) compared to T1 (74.46%). In progressive motility (PM) and progressive fast rectilinear velocity were higher (p<0.05) at T2 (48.65%; 41.44 μm/s) and T4 (55.38%; 41.45 μm/s) compared to T1 (45.54%; 37.01 μm/s). In conclusion, the different periods of artificial light did not influence testicular morphometry but did influence testosterone levels, sperm quality and testicular histology