Browsing by Author "Gavilanes Barbecho, Karol Vanessa"

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    Implementación de un protocolo de inseminación artificial por laparoscopía en ovejas en la granja de Irquis
    (Universidad de Cuenca, 2025-08-28) Gavilanes Barbecho, Karol Vanessa; Galarza Lucero, Diego Andrés
    This study evaluated the fertility of ewes artificially inseminated via laparoscopic technique using cryopreserved ram semen. Ten clinically healthy, reproductively sound adult ewes were selected and subjected to an estrus synchronization protocol. An intravaginal sponge impregnated with 50 mg of medroxyprogesterone acetate was inserted for 14 days, with a replacement on day seven. On day 14, the sponge was removed and 350 IU of equine chorionic gonadotropin (eCG) was administered. Laparoscopic artificial insemination (LAI) was performed 52 to 54 hours after eCG administration. The cryopreserved semen used for LAI was collected from two adult donor rams and frozen in 0.25 ml straws at a concentration of 100 x 10⁶ spermatozoa/ml. The LAI procedure was conducted under anesthesia using 2% xylazine, 10% ketamine, tiletamine, and zolazepam. Two incisions were made in the inguinal hypogastric region to introduce a light source through one port and a modified aspix device containing the thawed semen straw through the other. Semen was deposited into the uterine horn ipsilateral to the ovary bearing the preovulatory follicle. All ewes (10/10) responded to the synchronization protocol, as evidenced by behavioral estrus and the presence of a preovulatory follicle visualized by laparoscopy prior to insemination. Fertility was assessed via transrectal ultrasonography at 35 days post-LAI, yielding a conception rate of 70% (7/10). In conclusion, laparoscopic artificial insemination proved to be successful in achieving a 70% pregnancy rate. Increasing the number of inseminated ewes is recommended to validate these fertility results.