Browsing by Author "Curillo Santos, Diana Lorena"

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Caracterización de la comunidad de hongos endófitos asociados a la raíz de joyapa (Macleania rupestris) y evaluación del efecto de cepas cultivables en el desarrollo de plántulas
(Universidad de Cuenca, 2022-11-29) Curillo Santos, Diana Lorena; Peña Tapia, Denisse Fabiola
Macleania rupestris is a native Ericaceae with high potential in terms of benefits for health and the environment. Characterizing generalist or specialized interactions between M. rupestris and associated fungi is key to supporting its conservation and domestication. However, very little is known about the relationship between plant growth promoting endophytes and M. rupestris. Therefore, to learn more about the soil- associated microbiota of M. rupestris, we analyzed endophyte communities associated with the plant's surrounding soil and rhizosphere. Which presented a great abundance of genera such as Proteobacteria and Actinobacteria. rupestris plants. Objective: Characterize the endophytic fungal community associated with the root of joyapa (Macleania rupestris) and evaluate the effect of cultivable strains on seedlings development Methodology: Fungal endophytes were isolated from the roots of M. rupestris to evaluate their growth- promoting activity on seedlings. This research discusses the influence of endophytic fungi on the development of M. A total of six cultivable fungi were extracted, which were identified by the community of isolated fungi and characterized by molecular markers ITS (internal transcribed spacer) and LSU (large subunit). To investigate the effect of endophytes on plant growth, the 6 isolated fungi were selected to evaluate their ability to promote plant growth by applying a strain inoculation bioassay. Results: The results indicated that Clonostachys rosea and Trichoderma paravidescens isolated from the roots of M. rupestris have a vital role in promoting plant growth. Their potential use as inoculants to establish a sustainable crop production system and their application in the discovery of bioactive compounds should be part of future research.
Characterization of the microbial community associated with the roots of joyapa (Macleania rupestris) and the effect of fungal isolates on seedling developmentt
(2023) Cevallos Cevallos, Juan Manuel; Curillo Santos, Diana Lorena; Peña Tapia, Denisse Fabiola; Chica Martínez, Eduardo José
Macleania rupestris is a native Ericaceae with high potential benefits for health and the environment. Characterizing the interactions between M. rupestris and associated fungi is vital to supporting the plant's conservation and future domestication. However, little is known about the relationship between plant growth-promoting endophytes and M. rupestris. To learn more about the soil-associated microbiota of M. rupestris, we analyzed endophyte communities associated with the plant's rhizosphere and surrounding soil using nanopore sequencing. Additionally, fungal endophyte cultivable strains were isolated from the roots of M. rupestris to evaluate their growth-promoting activity on seedlings by applying a strain inoculation bioassay. Over 1000 genera were identified using nanopore sequencing, Bradyrhizobium and Mesorhizobium the most abundant in all samples analyzed. Similarly, six cultivable fungi were characterized by the molecular markers ITS (internal transcribed spacer) and LSU (large subunit). Amongst all isolates, Clonostachys rosea and Trichoderma paravidescens positively impacted seedlings' development. This study shows the potential of fungal strains as inoculants for the potential domestication of Macleania rupestris.
In Vitro-Based Production of Virus-Tested Babaco (Vasconcellea x heilbornii, syn. Carica pentagona) in Ecuador: An Integrated Approach to an Endangered Crop
(2023) Gómez Pinos, Sebastián Andrés; Curillo Santos, Diana Lorena; Muñoz Cali, Valeria Katherine; Quito Ávila, Diego Fernando; Sánchez Timm, Eduardo; Yánez Mendizábal, Viviana; Chica Martínez, Eduardo José; Peña Tapia, Denisse Fabiola; Moreno Peña, Lissette Delia
Babaco (Vasconcellea x heilbornii), a fruit-bearing vegetatively propagated crop native to Ecuador, is appreciated for its distinctive flavor and nutritional properties. The aim of this research was to determine a functional protocol for tissue culture propagation of virus-free babaco plants including in vitro establishment, multiplication, rooting, and acclimation. First, symptomless babaco plants from a single commercial nursery were analyzed for virus detection and cared for using different disinfection treatments in the greenhouse to reduce contamination during the in vitro establishing step, and three cytokinins, 6-(γ,γ-Dimethylallylamino) purine (2IP), 6-Benzylaminopurine (BAP), and Thidiazuron (TDZ), were used to determine the best hormone for multiplication. The best treatment for plant disinfection was the weekly application of copper sulfate at the greenhouse and a laboratory disinfection using ethanol (EtOH) (70%), Clorox (2%), and a solution of povidone iodine (2.5%), with an 80% survival during in vitro plant establishment. TDZ showed a better multiplication rate when compared with other hormones, and 70% of the rooted plants were successfully acclimated at the greenhouse. Generated plants were virus-free when tested against babaco mosaic virus (BabMV) and papaya ringspot virus (PRSV), two of the most important viruses that can affect babaco. An efficient protocol to produce virus-free babaco plants was elaborated with an integrated use of viral diagnostic tools to ensure the production of healthy start material to farmers.
Viability and infectivity of ceratobasidium sp. Encapsulated in alginate beads under different storage conditions
(2021) Lopez Alvarado, Queenny Kelly; Castro Añazco, Cesar Augusto; Curillo Santos, Diana Lorena; Chica Martínez, Eduardo José; Portilla Andrade, Jose Vicente; Peña Tapia, Denisse Fabiola
Mycorrhizal fungi are important partners of orchids because they establish close symbiotic relationships with this group of plants, and its preservation is also important for the successful conservation of orchids. In the present study, the conservation of Ceratobasidium sp., a fungal symbiont, using encapsulation in alginate beads was tested over different times, temperatures of storage and dehydrated conditions. Osmotically dehydrated and air-dried beads were stored at room temperature (20 ± 2 °C), 4 °C, −20 °C and −80 °C. The fungal growth was verified after 4, 8, 26 and 96 weeks. A second test was carried out to evaluate the encapsulations of fungi as a form of inoculation in Trichoceros antennifer orchid to promote symbiosis and plants development. The results show that the encapsulation of Ceratobasidium in alginate beads is a viable strategy for its conservation, the beads are of easy manipulation and promote plant growth when inoculated in plant substrate. These results may be adopted as part of effective conservation strategies for mycorrhizal fungi and orchids. © 2021. Universidad de Costa Rica.