Browsing by Author "Chuya Sigche, Erika Paulina"

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    Efecto de idebenona en la congelación y vitrificación de espermatozoides ovinos
    (Universidad de Cuenca, 2025-08-06) Chuya Sigche, Erika Paulina; Narváez Arcentales, María José; Soria Parra, Manuel Elías
    This study evaluated the effect of Idebenone (IDB) supplementation in conventional slow freezing (CC) and kinetic vitrification (VIT) media on the cryosurvival of ram spermatozoa. For that, eighteen ejaculates were collected from three adult rams (six/male) using an artificial vagina. Each ejaculate was diluted with TEST extender (tris, tes, glucose, and 6% egg yolk), supplemented with IDB (10 µM and 0 µM [control]), and cryopreserved using CF and VIT, establishing four treatments: CC+IDB, CC-Control, VIT+IDB, and VIT- Control. The CC was performed using 5% glycerol and exposing the samples to static liquid nitrogen (LN₂) vapor; while the VIT was done using 100 mM sucrose and direct immersion of 30 µL droplets into LN₂. The results showed that supplementation with IDB in the freezing medium (CC+IDB) significantly (P < 0.05) improved sperm kinematics and membrane integrity, and reduced DNA fragmentation post-thaw compared to its control (CC-Control). Additionally, IDB in the vitrification medium (VIT+IDB) significantly (P < 0.05) reduced oxidative stress and DNA fragmentation compared to VIT-Control. Nevertheless, VIT+IDB samples showed a significant (P < 0.05) undesirable decrease in several kinematic parameters (velocities, linearity, and oscillation) after warming compared to VIT-Control. In conclusion, IDB improved kinematics, viability, and acrosomal integrity, and reduced DNA fragmentation in frozen-thawed ram spermatozoa. Despite its undesirable effect on post-warming kinematics, IDB reduced oxidative stress and DNA fragmentation following the vitrification process.