Browsing by Author "Castro Urdiales, Dario Vinicio"

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    Composición fitoquímica de los aceites esenciales de Syzygium aromaticum y Citrus limonia y actividad antimicrobiana frente a Staphylococcus aureus subsp. aureus cepa ATCC 25923
    (Universidad de Cuenca, 2020-02-26) Castro Garzón, Leonardo Enrique; Castro Urdiales, Dario Vinicio; Carchipulla Sanango, Claudia Janneth; Cuzco Quizhpi, Nancy Mirian
    Currently, science has launched a research boom through the return in history to study plant species with potential biological activities, being the main antimicrobial property of interest, in order to counteract many problems related to pathogenic microorganisms, especially those capable of developing resistance mechanisms against usual antimicrobial treatments such as Staphylococcus aureus. In this project an exploratory and quasi-experimental study was conducted in which the antimicrobial activity of the essential oils (AEs) of the plant species Citrus limonia and Syzygium aromaticum against the Staphylococcus aureus ATCC 25923 bacteria was determined. The oils essential were obtained by steam traction, obtaining a yield of 0.64% for Citrus limonia and 1.19% for Syzygium aromaticum, subsequently the main bioactive components were identified by TLC based on their retention factors and colorimetric characteristics, demonstrating the presence of Limonene, Citral, Geraniol, Trans-Caryophylene, Alpha-Pinene, Alpha-Terpinen and Eugenol in the Citrus Limon AE and Eugenol in Syzygium aromaticum; finally, the antimicrobial activity of the AEs was determined against the Staphylococcus aureus strain ATCC 25923 by means of the standardized method of macrodilution in broth, obtaining a MIC of 0.6% for the Citrus limonia AE and 0.3% for Syzygium aromaticum; and a CMB using the agar extension technique of 2.5% and 1.25% respectively.