Browsing by Author "Carchipulla Sanango, Claudia Janneth"

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Composición fitoquímica de los aceites esenciales de Syzygium aromaticum y Citrus limonia y actividad antimicrobiana frente a Staphylococcus aureus subsp. aureus cepa ATCC 25923
(Universidad de Cuenca, 2020-02-26) Castro Garzón, Leonardo Enrique; Castro Urdiales, Dario Vinicio; Carchipulla Sanango, Claudia Janneth; Cuzco Quizhpi, Nancy Mirian
Currently, science has launched a research boom through the return in history to study plant species with potential biological activities, being the main antimicrobial property of interest, in order to counteract many problems related to pathogenic microorganisms, especially those capable of developing resistance mechanisms against usual antimicrobial treatments such as Staphylococcus aureus. In this project an exploratory and quasi-experimental study was conducted in which the antimicrobial activity of the essential oils (AEs) of the plant species Citrus limonia and Syzygium aromaticum against the Staphylococcus aureus ATCC 25923 bacteria was determined. The oils essential were obtained by steam traction, obtaining a yield of 0.64% for Citrus limonia and 1.19% for Syzygium aromaticum, subsequently the main bioactive components were identified by TLC based on their retention factors and colorimetric characteristics, demonstrating the presence of Limonene, Citral, Geraniol, Trans-Caryophylene, Alpha-Pinene, Alpha-Terpinen and Eugenol in the Citrus Limon AE and Eugenol in Syzygium aromaticum; finally, the antimicrobial activity of the AEs was determined against the Staphylococcus aureus strain ATCC 25923 by means of the standardized method of macrodilution in broth, obtaining a MIC of 0.6% for the Citrus limonia AE and 0.3% for Syzygium aromaticum; and a CMB using the agar extension technique of 2.5% and 1.25% respectively.
Control de calidad microbiológica de morcillas de cerdo blanca y negra expendidas en espacios públicos de la ciudad de Cuenca
(2019-01-31) Pineda Castro, Gabriela Estefanía; Esthela Viviana, Quilli Nieves; Carchipulla Sanango, Claudia Janneth
The existence of public spaces where morcillas (blood sausages) are sold, and the lack of measures to control their microbiological quality can give rise to foodborne diseases (FBD), which in turn represents a major health problem for consumers in the future. For this research we determined the microbiological quality of black and white pork blood sausages, which are sold in several public spaces around the city of Cuenca. Through application of the NTE INEN 1338:2012 standard and the Peruvian Technical Standard for black and white blood sausage, respectively, both of which establish microbial limits for the microorganisms under study, thus ensuring that these products are suitable for commercialization. An observational, descriptive, cross-sectional study was conducted. Ten duplicate samples obtained randomly from several points of sale were analyzed and determined by Compact Dry plates, Mesophyll aerobes, Escherichia coli and Staphylococcus aureus, while the Reveal 2.0 kit was used to determine Salmonella spp and Listeria monocytogenes, lastly the method described in the Bacteriological Analytical Manual (BAM), Chapter 16, was used for determination of Clostridium perfringens. The results obtained from the microbiological analysis were statistically analyzed, for this reason it was possible to determine that for black and white blood sausage most microbiological parameters comply with current regulations. Despite this, a comprehensive training regarding Good Handling Practices (GHP) was provided together with the City's Urban Control Department
Evaluación de la eficacia del desinfectante líquido alcalino clorado (ACL 40) frente a coliformes totales y E. coli en las superficies en contacto con alimentos en el área de producción de la empresa New Lac
(2019-11-07) Gavilanes Rodas, Juan Diego; Vásquez Peralta, Sergio Esteban; Carchipulla Sanango, Claudia Janneth
The efficacy of the chlorinated alkaline liquid disinfectant ACL 40 in 5% concentration for 30 minutes of exposure time, was evaluated against total coliforms and E. coli on regular and irregular inert surfaces in contact with food in the production area of the company New Lac. This study was based on a descriptive, observational, cross-sectional research. The sampling was carried out by means of the hyssop method. A total of 240 samples were sampled for later analysis by 3M® Petrifilm Plates: E. coli / Coliforms. In the statistical analysis, the Wilcoxon-Mann-Whitney test was used, because the results were non-parametric. The alternative hypothesis was accepted: "The population of total coliforms and E. coli before the PLD is greater than the population of total coliforms and E. coli after the PLD." However, to show the dispersion of the microbiological counts, histograms were performed and to demonstrate the microbicidal action of the disinfectant, box plots were used, which allowed comparing the bacterial populations of both total coliforms and E. coli before and after the PLD. Finally, the colony count of total coliforms where the significant reduction of the population of total coliforms was observed in most of the regular and irregular inert surfaces, complying with the rule. In the case of E. coli, absence was reported in the microbiological counts, achieving the elimination of the population and thus complying with the regulations, which requires the absence of the microorganism in question.
Evaluación in vitro de la actividad antibacteriana de los extractos etanólicos de Uncaria tomentosa; Piper aduncun; Artemisa absinthium frente a Escherichia coli ATCC 25922
(2020-02-26) Guayas Guamán, Jonnathan Paul; Lazo Burga, Patricio Israel; Carchipulla Sanango, Claudia Janneth
The plants have been used traditionally for medicinal purposes and could constitute a valuable resource to find new molecules with pharmacological activity in the face of conditions of microbiological origin, especially with the bacterial type. Those that currently constitute a public health problem, mainly due to the resistance acquired from microorganisms to the drugs used in their usual treatment and other factors that contribute to the emergence of resistant bacteria. The purpose of this research was the in-vitro evaluation of the antibacterial activity of ethanol extracts of ethanol plants of Uncaria tomentosa (cat's claw), Piper aduncum (matico), and Artemisa absinthium (wormwood) against the strain of E Coli ATCC 25922. The plant extracts were obtained through the maceration method using 96% ethanol as a solvent. The main functional groups present in the extracts obtained were identified by specific qualitative reactions to confirm the species and subsequently, the antibacterial activity was evaluated by the method of microdilution on the plate standardized by Clinical and Laboratory Standards Institute (CLSI) M100 29 to determine the minimum inhibitory concentration (MIC) of alcoholic extracts against E. coli. The research was exploratory and experimental. It shows the following results: Uncaria tomentosa had a minimum inhibitory concentration (MIC) at 625 µg/ml, while the case of Artemisia absinthium and Piper aduncum no bactericidal effect was observed; however, a slight decrease was observed in bacterial growth so it could arise to hurt that these plants have a bacteriostatic effect.
Prevalencia de micosis superficiales en adultos mayores de centros gerontológicos residenciales y de atención diurna de la ciudad de Cuenca durante el período 2014-2018
(Universidad de Cuenca, 2020-03-05) Lima Coronel, Eliana Vanessa; Siguenza Trelles, Viviana Stefanía; Carchipulla Sanango, Claudia Janneth; López Cisneros, Carmen Lucía
Introduction: Superficial mycoses are a group of infections that affect a considerable number of individuals, including children and the elderly, the latter presenting age-specific characteristics that make them susceptible to acquiring diseases in general, including superficial mycoses. Most of the older adults are isolated in specialized centers so the study is carried out in gerontological centers providing daytime and residential care in the city of Cuenca whose patients participated in the project of the Faculty of Chemical Sciences. General objective: To determine the prevalence of superficial mycosis in gerontological centers of diurnal and residential attention in the city of Cuenca, during the period 2014 - 2018. Methodology: This research was quantitative - descriptive, cross-sectional and retrospective, the study population included 417 elderly patients, data like: age, sex, fungal lesions and etiological agents diagnosed in the project was collected. Results: A 72.5% of positivity was obtained, 74.1% were women, the most affected age group was over 80 years old (53.2%), with the frequency rates being at 40% for blastomycetes, 36% for dermatophytes and 24% for other filamentous fungi; the most frequent locations were the lower extremities (75.8%), followed by the head and neck area (16.3%); the most prevalent etiological agents were Candida albicans (10%), Trichophyton tonsurans (8%) and Candida glabrata - Trichophyton mentagrophytes (7%).