Browsing by Author "Caguana Mayancela, John Patricio"

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    Caracterización de los genotipos frecuentes del virus del papiloma humano en mujeres atendidas en los hospitales Monte Sinai y del Seguro Social Cuenca- Ecuador. 2008- 2014
    (2017) Arcentales Cayamcela, Mauro Javier
    OBJECTIVE: To determine the prevalence of hu- man papilloma virus genotypes in cervical-uterine samples and their relation to risk factors in women with active sex life in Cuenca. METHODOLOGY: It is a cross-sectional observatio- nal study with a sample of 500 women from Cuen- ca who were attended in the Monte Sinai Hospi- tal and the Ecuadorian Institute of Social Security (IESS). The exams were carried out in the BIONCO- GEN Molecular Biology Laboratory which is loca- ted in Cuenca. The Real Time PCR technique plus the Pap examination were used. RESULTS: The prevalence of HPV was 78.4 % and the most frequent genotype was the HPV 16 with 26.2 %. On the other hand, the CIN I with 27% is the most frequent gynecological pathology. CONCLUSION: The genotypes 16/18 represent the types of HPV that are directly related to the de- velopment of cervical cancer because when it is correlated with cytology (Pap) it is demonstrated that the cell undergoes morphological changes (koilocytosis).
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    Caracterización de los genotipos frecuentes del virus del Papiloma Humano en mujeres atendidas en los hospitales Monte Sinai y del Seguro Social Cuenca- Ecuador. 2008- 2014
    (Universidad de Cuenca. Facultad de Ciencias Médicas, 2017-04) Campoverde Cisneros, Manuel Alfredo; Arcentales Cayamcela, Mauro; Caguana Mayancela, John Patricio
    OBJECTIVE: To determine the prevalence of human papilloma virus genotypes in cervical-uterine samples and their relation to risk factors in women with active sex life in Cuenca. METHODOLOGY: It is a cross-sectional observational study with a sample of 500 women from Cuenca who were attended in the Monte Sinai Hospital and the Ecuadorian Institute of Social Security (IESS). The exams were carried out in the BIONCOGEN Molecular Biology Laboratory which is located in Cuenca. The Real Time PCR technique plus the Pap examination were used. RESULTS: The prevalence of HPV was 78.4 % and the most frequent genotype was the HPV 16 with 26.2 %. On the other hand, the CIN I with 27% is the most frequent gynecological pathology. CONCLUSION: The genotypes 16/18 represent the types of HPV that are directly related to the development of cervical cancer because when it is correlated with cytology (Pap) it is demonstrated that the cell undergoes morphological changes (koilocytosis). Keywords: Genotype, Human papillomavirus 16, Human papillomavirus 18, Clinical Laboratory Techniques, PCR; Risk factors; Monte Sinai Hospital, Hospital of the Ecuadorian Institute of Social Security, Cuenca-Ecuador.
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    Diagnóstico de enfermedades de transmisión sexual mediante PCR tiempo real en mujeres indígenas del Ecuador 2016
    (Universidad de Cuenca, 2017-11-11) Caguana Mayancela, John Patricio; Carreño Calle, Christian Santiago; Campoverde Cisneros, Manuel Alfredo
    Background Screening test for the detection of sexually transmitted diseases, some of them producing cancer such as human papilloma virus, besides being factors of infertility and infertility, depends increasingly on molecular techniques. An important challenge for developing countries like Ecuador has been the lack of availability and costs of tests based on the detection of the DNA of the microorganism. In this study, microorganisms of sexual transmission were diagnosed by qPCR in women of indigenous nationality Ecuador. Methodology Inside the laboratory of molecular biology of the Cuenca University, a descriptive study was carried out to diagnose sexually transmitted microorganisms in cervical samples using molecular techniques of HPV-28 and STI-7 Anyplex qPCRs. The samples came from woman indigenous from the provinces of Loja, Morona Santiago and Cañar. Results 396 samples were analyzed, 120 samples were extracted in Saraguro, 131 in Quilloac community, 145 in Seville Don Bosco community. 135 samples showed high risk and low oncogenic HPV risk genotypes, also 362 samples with other sexually transmitted microorganisms. Conclusions Implementing molecular techniques such as qPCR, for the diagnosis of microorganisms of sexual transmission are fast, simple with a high sensitivity and specificity