Browsing by Author "Bravo Barbecho, Edisson Homero"

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Optimización del modelo de pez cebra (Danio rerio) para toxicidad de extractos vegetales
(Universidad de Cuenca, 2022-10-11) Peralta Córdova, Esteisy Paulina; Bravo Barbecho, Edisson Homero; Peñaherrera Wilches, María Eugenia
The present project focused on determining the toxicity of two methanolic and ethanolic extracts of Ruta graveolens (Ruda) and Phyllanthus anisolobus (Barbasco) respectively, using fish embryo toxicity tests (FET) with the zebrafish (Danio rerio) as a model organism. The study began with the selection of two plants that have reported toxicity both in studies carried out within the "Plant Group of the Biosciences Department of Cuenca University" and in bibliographic information, which were collected, botanically characterized, washed, dried and concentrated to obtain the dry extracts. On the other hand, for the FET, as a first step, the fish tanks were adapted following the guidelines described in the OECD manual in order to obtain a good performance in egg laying, which were then put in contact with the extracts at different concentrations in order to find toxicity based on lethality, which was observed in each test and used to calculate the LC50. The obtained results were analyzed statistically with the use of Microsoft Excel 2016- 64 bits using a linear regression for the calculation of the LC50 of each extract, with which it was concluded that both extracts present toxicity at low concentrations, being the ethanolic extract of Phyllanthus anisolobus (Barbasco) the one that presented a lower LC50 and therefore presented a higher toxicity.