Browsing by Author "Bermeo Bacuilima, Melida Maribel"

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    Evaluar la sincronización de celo con PG y su efecto estimado sobre la fertilidad y prolificidad en cobayas nativas
    (Universidad de Cuenca, 2022-09-23) Bermeo Bacuilima, Melida Maribel; Guamán Guallpa, Anabel Gabriela; Rosales Jaramillo, Cornelio Alejandro
    The native guinea pig is an autochthonous resource of the Country with unique characteristics of rusticity and adaptability; however, these native subpopulations have been affected by the introduction of improved lines, leading to the loss of local genetics, for which there is a need to implement reproductive biotechnologies that allow safeguarding this valuable genetic material, but to apply these techniques it is It is necessary first to control the estrous cycle of the female. For this reason, the objective of this research was to evaluate estrus synchronization with PGF2α, its estimated effect on fertility and prolificacy in primiparous native guinea pigs, whose purpose was to establish an efficient time interval that allows estrus synchronization. For this, 32 native guinea pigs with a similar weight and age (449,13 ± 31,6 g and 3 months) were used, randomly distributed in 4 treatments of 8 animals each. Prior to estrus synchronization, the estrous cycle was determined by opening the vaginal membrane (AM) and vaginal cytology with Wright's stain. On the other hand, for estrus synchronization the following treatments were used: control T1 without PGF2α; T2 0,04 mg of PGF2α day 0 and day 7; T3 0,04 mg PGF2α, day 0 and day 9; T4 dose of 0,04 mg of PGF2α, day 0 and day 11; estrus synchronization efficiency was evaluated through AM, fertility rate and prolificacy. Thus, an estrous cycle duration of 15,82 ± 0,57 days was determined, distributed in: proestrus 33 ± 5,2 hours, estrus 8,31 ± 0,7 hours, metestrus 33,37 ± 5 hours and right-handed 12,31 ±0,6 days. Besides, it was shown that the effective estrus synchronization treatment was T4, with 100% AM compared to T2 and T3 that did not exhibit AM and T1 exhibited 37,5% AM. The fertility and prolificacy rate in T4 was 87,5% and 2.57 pups/female. It is concluded that the application of 0,04 mg of PGF2α on days 0 and 11 was efficient to synchronize oestrus, exhibiting satisfactory fertility and prolificacy rates in primiparous native guinea pigs.