Browsing by Author "Becerra Navarrete, Edgar Antonio"

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Detección de la mutación JAK2 V617F en neoplasias mieloproliferativas en población ecuatoriana por reacción en cadena de la polimerasa alelo específica
(2017) Campoverde Cisneros, Manuel Alfredo; Oliveros Alvear, Jorge William; Reyes Peña, Ines Maria; Maldonado Guerrero, Bella Irma; Becerra Navarrete, Edgar Antonio; Ullauri Zambrano, Veronica Alexandra; Villa Cardenas, Esteban Homero; Espinoza Calle, Glenda Iralda; Ladines Castro, Washington Javier; Chacon Velez, Jennifer Yadira; Arcentales Cayamcela, Mauro Javier
In 2005, five independent research groups reported the association of V617F mutation in the gene encoding the janus kinase 2 (JAK2) Myeloproliferative Neoplasms Small (NMP), including polycythemia vera (PV), Essential thrombocytosis (ET) and primary myelofibrosis (MFP). This study aimed to detect the JAK2 V617F mutation in Ecuadorian patients through chain reaction polymerase allele specific (AS-PCR). The research design was descriptive cross-sectional and included a total of 40 patients, 20 with NMP and 20 controls attending the various services Haematology Health Institutions participating. Genetic material was extracted and then amplified by AS-PCR, while the clinical hematology of NMP was performed by using specialized clinical, morphological and hematological tests such as blood counts and flow cytometry examination hematologists. The frequency of the JAK2 V617F mutation found was 50% with a rate of 95% confidence. Patients with a greater presence of the mutation (35%) were those with PV, followed by patients with TE (15%), the average age being 45. These data confirm that the JAK2 V617F mutation is prevalent in NMP and has become the molecular marker of both the PV, ET and MFP. Likewise, this study has shown that the AS-PCR method is a quick, easy and cost-effective diagnosis.
Frecuencia de hemorragia subaracnoidea por rotura de aneurisma en el H.V.C.M. Cuenca, 1990-1994
(1995) Bailón Pico, Bawer Axdud; Becerra Navarrete, Edgar Antonio; Benítez Serrano, Ximena Esperanza; Díaz Heredia, Favian Eduardo
This is a descriptive epidemiological study in 26 patients diagnosed by clinical and complementary examinations of subarachnoid hemorrhage from ruptured aneurysm in the Vicente Corral Moscoso Regional Hospital, from January 1990 to December 1994 the frequency of subarachnoid hemorrhage due to rupture was determined aneurysm, and the main features according to age, sex, underlying cause, clinical features frequently used type of treatment in the hospital, evolution and complications and determine the neurological sequelae are described. After identifying the case it proceeded in each clinic to apply the relevant questionnaire previously developed as an instrument of data collection history. We conclude that the frequency of subarachnoid hemorrhage from ruptured aneurysm is 8.1 per thousand neurological cases per year, predominantly in females with an average age for women from 38.9 years for men and 48.18 years. The precipitating causes, is related to daily activities (53.84%) and physical effort (26.92%). The most common clinical symptoms and signs include headache, sudden loss of consciousness, vomiting and meningeal signs. The most common complication is vasospasm (53.84%). 57.4% in the surgery was performed. Among the neurological sequelae are hemiparesis, hemiplegia, relevant recommendations are listed