Browsing by Author "Arcentales Cayamcela, Mauro Javier"

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Caracterización de los genotipos frecuentes del virus del papiloma humano en mujeres atendidas en los hospitales Monte Sinai y del Seguro Social Cuenca- Ecuador. 2008- 2014
(2017) Arcentales Cayamcela, Mauro Javier
OBJECTIVE: To determine the prevalence of hu- man papilloma virus genotypes in cervical-uterine samples and their relation to risk factors in women with active sex life in Cuenca. METHODOLOGY: It is a cross-sectional observatio- nal study with a sample of 500 women from Cuen- ca who were attended in the Monte Sinai Hospi- tal and the Ecuadorian Institute of Social Security (IESS). The exams were carried out in the BIONCO- GEN Molecular Biology Laboratory which is loca- ted in Cuenca. The Real Time PCR technique plus the Pap examination were used. RESULTS: The prevalence of HPV was 78.4 % and the most frequent genotype was the HPV 16 with 26.2 %. On the other hand, the CIN I with 27% is the most frequent gynecological pathology. CONCLUSION: The genotypes 16/18 represent the types of HPV that are directly related to the de- velopment of cervical cancer because when it is correlated with cytology (Pap) it is demonstrated that the cell undergoes morphological changes (koilocytosis).
Detección de la mutación JAK2 V617F en neoplasias mieloproliferativas en población ecuatoriana por reacción en cadena de la polimerasa alelo específica
(2017) Campoverde Cisneros, Manuel Alfredo; Oliveros Alvear, Jorge William; Reyes Peña, Ines Maria; Maldonado Guerrero, Bella Irma; Becerra Navarrete, Edgar Antonio; Ullauri Zambrano, Veronica Alexandra; Villa Cardenas, Esteban Homero; Espinoza Calle, Glenda Iralda; Ladines Castro, Washington Javier; Chacon Velez, Jennifer Yadira; Arcentales Cayamcela, Mauro Javier
In 2005, five independent research groups reported the association of V617F mutation in the gene encoding the janus kinase 2 (JAK2) Myeloproliferative Neoplasms Small (NMP), including polycythemia vera (PV), Essential thrombocytosis (ET) and primary myelofibrosis (MFP). This study aimed to detect the JAK2 V617F mutation in Ecuadorian patients through chain reaction polymerase allele specific (AS-PCR). The research design was descriptive cross-sectional and included a total of 40 patients, 20 with NMP and 20 controls attending the various services Haematology Health Institutions participating. Genetic material was extracted and then amplified by AS-PCR, while the clinical hematology of NMP was performed by using specialized clinical, morphological and hematological tests such as blood counts and flow cytometry examination hematologists. The frequency of the JAK2 V617F mutation found was 50% with a rate of 95% confidence. Patients with a greater presence of the mutation (35%) were those with PV, followed by patients with TE (15%), the average age being 45. These data confirm that the JAK2 V617F mutation is prevalent in NMP and has become the molecular marker of both the PV, ET and MFP. Likewise, this study has shown that the AS-PCR method is a quick, easy and cost-effective diagnosis.
Reacción en cadena de polimerasa transcriptasa inversa para diagnóstico de leucemia mieloide crónica Cuenca 2013-2017
(Universidad de Cuenca, 2018-11-11) Arcentales Cayamcela, Mauro Javier; Peña Patiño, Sandra Gioconda
Background: The Reverse Transcriptase Polymerase Chain Reaction (RT-PCR), a tool for important diagnosis in the detection of reciprocal translocations between chromosomes, which consists of using ribonucleic acid to generate multiple copies of a specific region through the enzyme Taq polymerase. General objective: General Objective: To determine the prevalence of the BCR / ABL p210 fusion gene by means of the Reverse Transcriptase Polymerase Chain Reaction (RT-PCR) technique in patients with Chronic Myeloid Leukemia (CML). Methodology: Methodology: The design of the research was observational, descriptive and retrospective, carried out from December 2013 to December 2017 and included a total of 121 patients with a diagnosis of CML. The results obtained were analyzed and tabulated with the software SPSS version 23.0 and Excel 2016; they are represented in tables and graphs. Results: The prevalence of the BCR / ABL p210 fusion gene was 38.84% of which positive cases in the city of Cuenca were 24,79 %, male patients accounted for 30,58 % and 9,92 % made up the age group of 28- 37 years. Conclusion: The patients with greater predisposition to the disease were the young adults of the male sex, the city of Cuenca presented the highest number of positive cases and the implementation of the RT-PCR technique was the highly sensitive in vitro diagnostic method for the detection of the gene of fusion BCR / ABL p210
Variantes de cepas de COVID-19
(Red Editorial Latinoamericana de Investigación Contemporánea, 2022) Torres Criollo, Larry Miguel; Campoverde Cisneros, Manuel Alfredo; Vera Siguenza, Juan Sebastián; Arévalo Jara, Jonnathan Fabricio; Ycaza Zurita, Maria Gabriela; Arcentales Cayamcela, Mauro Javier
lntroduction. For the first time, the 2019 corona virus (COVID-19) was detected in China at the end of 2019 and was declared a pandemic on March 11, 2020. As it is a virus with a high potential far mutation, it evolves into very aggressive strains and others less, this is evidenced in multiple investigations, currently the treatment is based on multiple therapies to prevent respiratory problems, one of the most efficient forms is antiviral therapy and vaccination. Objective. lnvestigate the main variants and characteristics of the SARS-CoV-2 virus, as well as describe each of them and how they present certain variants, through a bibliographic review, based on articles, magazines and scientific publications.Methodology. This research is of an explanatory-descriptive nature, based on the consultation of multiple internationally renowned bibliographic sources in the period from May 2019 to September 2022. Results. 31 articles of scientific quality were analyzed and paraphrased and the necessary information on the different variants, frequency, and pathogenicity of COVID 19 was collected. Conclusions. Concern Variants significantly increase transmissibility and have the potential to increase transmission and severity of disease. They may also affect the efficacy of adenovirus and mRNA vector vaccines, although currently licensed COVID-19 vaccines remain effective in preventing serious infection and disease. lf a variant emerges with a more significant impact on global public health, it would pose a greater threat to humanity; therefore, measures to reduce transmission of the virus and efforts to monitor and understand the impact of variants must continue.