Browsing by Author "Andrade Herrera, Juan Sebastian"

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    Producción y modelamiento de bioetanol obtenido a partir de los residuos de cacao mediante hidrólisis enzimática y fermentación anaerobia
    (Universidad de Cuenca, 2024-11-26) Andrade Herrera, Juan Sebastian; Delgado Noboa, Jorge Washington
    The objective of this study was to obtain bioethanol from cocoa husk using enzymatic hydrolysis with two types of enzymes and alcoholic fermentation with Saccharomyces cerevisiae. Additionally, a kinetic modeling of bioethanol production was conducted using the Modified Gompertz model in Matlab. A two-factor experimental design was implemented, with cellulase concentration (Cellulase from Trichoderma reesei-Celluclast) at three levels (0.1, 0.2, and 0.3 ml/g) and hemicellulase concentration (Hemicellulase from Aspergillus niger H2125) at two levels (0.1 g/g and 0.3 g/g). A two-factor ANOVA was performed to analyze the data. The results indicated that the optimal concentration for sugar production was 0.3 ml/g of cellulase and 0.3 g/g of hemicellulase, although no significant difference was observed between the use of 0.1 and 0.3 ml/g of cellulase. Additionally, using 0.3 g/g of hemicellulase reduced the hydrolysis time to 24 hours. The final ethanol concentration obtained was 90.59 grams of bioethanol per kilogram of dry biomass. Finally, the kinetic modeling in Matlab showed a similarity between the experimental data and the model, which revealed that fermentation was completed at approximately 12 hours