Cambios en comunidades microbianas de la rizósfera de Solanum betaceum frente enriquecimiento de la microbiota edáfica nativa con consorcios microbianos

Abstract

The use of native or introduced microorganisms represents one of the alternatives to the use of synthetic chemical products in agriculture since they can be applied as biological inoculants (bioinoculants), providing the enrichment of soil microbial communities. However, despite the fact that they are used to improve crop productivity, there is still little information about their effects on microbial communities in the rhizosphere of crops and their monitoring during their establishment and/or post-inoculation is not reported. Therefore, by means of metagenomics, it was determined whether the inoculation with beneficial microorganisms induces changes in the diversity of the microbial communities residing in the rhizosphere of S. betaceum., in addition to the estimation of the persistence of Trichoderma spp., with the use of a specific PCR protocol. As a result, no significant changes were reported in the structure of the microbial community as a consequence of bioinoculation, despite the evidence of a slight difference between the control and treatment samples, which cannot be directly attributed to the application of the bioinoculant. When evaluating the persistence of Trichoderma spp., its establishment in the rhizosphere of the crop was not evidenced after 120 days after inoculation. However, the present study provides preliminary and, until now, pioneering information about the microbial communities present in the rhizosphere of S. betaceum, analyzing their diversity and monitoring after inoculation. As a result, biotechnological advances can be made in conjunction with a comprehensive understanding of genetic diversity, dynamics of microbial communities, and a greater understanding of the microbial profiles of the rhizosphere of this fruit, which is characterized by having an important nutritional value and, in recent years, being considered as an alternative in the production, diversification, and exploitation.