Efecto del resveratrol sobre la capacidad fecundante in vitro de espermatozoides bovinos congelados y vitrificados

Abstract

This research evaluated the effect of Resveratrol (RES) on the kinematics and in vitro fertilizing capacity of frozen and vitrified bovine spermatozoa. Four treatments were evaluated according to RES supplementation (0 [control] and 50 µM) and cryopreservation method (conventional freezing [CF] and vitrification [VIT]): CF-RES (n=80 straws); CF-Co (n=80 straws); VIT-RES (n=20 cryotubes), and VIT-Co (n=20 cryotubes). Each treatment was objectively evaluated for its kinematics and fertilizing capacity using a Computer Assisted Sperm Analysis system (CASA®) and in vitro fertilization (200 oocytes/treatment), respectively. Results showed better cryoresponse kinematics with freezing treatments (CFRES and CF-Co) than with vitrification treatments (VIT-RES and VIT-Co). The CF-RES treatment produced a higher (P<0.05) total motility (TM) and progressive motility (PM) than the CF-Co treatment, while the VIT-RES treatment produced a higher (P<0.05) beat cross frequency (BCF) than the VIT-Co treatment. The CC-RES treatment produced a higher (P<0.05) cleavage rate than the CF-Co and VIT- RES treatments (64.0±3.40% vs. 34.7±3.44% and 42.0±4.96%, respectively). Likewise, the CF-RES treatment produced a higher percentage of blastocysts (P<0.05) than all treatments (CF-RES=22.5±3.11% vs. CCCo=10.2±2.29%; VIT-RES=8.0±3.88%; and VIT-Co=4.0±2.8%). Furthermore, a correlation (P<0.05) was evidenced in the CC-RES treatment between motilities and lateral head displacement amplitude, and cleavage and blastocyst rates. In conclusion, RES improved the motility and in vitro fertility of spermatozoa after freezing, however, in vitrification it only improved one kinetic parameter.