Title: | Methods of collection, extender type, and freezability of semen collected from creole bulls raised in the tropical highlands of Ecuador |
Other Titles: | Métodos de recolección, tipo extensor y congelación del semen recolectado de toros criollos criados en las tierras altas tropicales de Ecuador |
Authors: | Argudo Garzon, Daniel Ernesto Galarza Lucero, Diego Andres Bueno Leon, Hernan Patricio Iñiguez Gutierrez, Carlos Ulises Mendez Alvarez, Maria Silvana Soria Parra, Manuel Elias Torres Inga, Carlos Santiago Perea Ganchou, Fernando Pedro Alberio Null, Ricardo Horacio |
metadata.dc.ucuenca.correspondencia: | Argudo Garzon, Daniel Ernesto, daniel.argudo@ucuenca.edu.ec |
Keywords: | Creole bull Andean highland Electroejaculation Artificial vagina Soybean- and egg yolk- based extender Cryopreservation |
metadata.dc.ucuenca.areaconocimientofrascatiamplio: | 1. Ciencias Naturales y Exactas |
metadata.dc.ucuenca.areaconocimientofrascatidetallado: | 1.6.9 Biología de la Reproducción |
metadata.dc.ucuenca.areaconocimientofrascatiespecifico: | 1.6 Ciencias Biológicas |
metadata.dc.ucuenca.areaconocimientounescoamplio: | 08 - Agricultura, Silvicultura, Pesca y Veterinaria |
metadata.dc.ucuenca.areaconocimientounescodetallado: | 0841 - Veterinaria |
metadata.dc.ucuenca.areaconocimientounescoespecifico: | 084 - Veterinaria |
Issue Date: | 2019 |
metadata.dc.ucuenca.embargoend: | 30-Dec-2050 |
metadata.dc.ucuenca.volumen: | volumen 51, número 7 |
metadata.dc.source: | Tropical Animal Health and Production |
metadata.dc.identifier.doi: | 10.1007/s11250-019-01877-3 |
metadata.dc.type: | ARTÍCULO |
Abstract: | This study was conducted to determine the best combination between two collection method and two extenders in the cryopres-ervation of semen from creole bulls adapted to highlands of the Ecuadorian Andes. Sixty ejaculates from three adult Creole bulls were evaluated after collection by artificial vagina (AV) and electroejaculation (EE). Semen samples were split into two aliquots and diluted with a soy lecithin extender (Andromed®; A) or an egg yolk-containing extender (Triladyl®; T) and packed in straws of 0.25 ml with 20 × 106 sperms. Optical microscopy and computer-assisted semen analysis system (CASA) were used to evaluate semen quality characteristics. The effects of collection methods and extender type as well as its interaction were evaluated by a factorial ANOVA and Bonferroni’s test. Semen samples collected with EE and frozen with T (EE-T) and A (EE-A) had greater proportion of spermatozoa with optical assessed individual progressive motility (IPM), plasmatic membrane intact (HosT), and lower tail abnormalities than those obtained with AV and frozen with the same extenders (AV-T and AV-A);however, differences were significant only between EE-A and AV-T. CASA assessment indicated that the total mobility (TM) was greater (P < 0.05) in semen samples diluted with T, although these samples had a greater proportion (P < 0.05) of sperms with local motility (LM) and fewer immobile sperms (IS), than those extended with A. Generally, semen samples obtained with EE or AV and diluted with T seems to be the best option to ciopreserve gametes of Creole bulls raised in highlands of Ecuadorian Andes. |
Description: | This study was conducted to determine the best combination between two collection method and two extenders in the cryopres-ervation of semen from creole bulls adapted to highlands of the Ecuadorian Andes. Sixty ejaculates from three adult Creole bulls were evaluated after collection by artificial vagina (AV) and electroejaculation (EE). Semen samples were split into two aliquots and diluted with a soy lecithin extender (Andromed®; A) or an egg yolk-containing extender (Triladyl®; T) and packed in straws of 0.25 ml with 20 × 106 sperms. Optical microscopy and computer-assisted semen analysis system (CASA) were used to evaluate semen quality characteristics. The effects of collection methods and extender type as well as its interaction were evaluated by a factorial ANOVA and Bonferroni’s test. Semen samples collected with EE and frozen with T (EE-T) and A (EE-A) had greater proportion of spermatozoa with optical assessed individual progressive motility (IPM), plasmatic membrane intact (HosT), and lower tail abnormalities than those obtained with AV and frozen with the same extenders (AV-T and AV-A);however, differences were significant only between EE-A and AV-T. CASA assessment indicated that the total mobility (TM) was greater (P < 0.05) in semen samples diluted with T, although these samples had a greater proportion (P < 0.05) of sperms with local motility (LM) and fewer immobile sperms (IS), than those extended with A. Generally, semen samples obtained with EE or AV and diluted with T seems to be the best option to ciopreserve gametes of Creole bulls raised in highlands of Ecuadorian Andes. |
URI: | http://dspace.ucuenca.edu.ec/handle/123456789/33948 https://link.springer.com/article/10.1007%2Fs11250-019-01877-3 |
metadata.dc.ucuenca.urifuente: | https://link.springer.com/journal/11250/51/7 |
ISSN: | 0049-4747, e 1573-7438 |
Appears in Collections: | Artículos
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