Susceptibilidad antimicrobiana de cepas de bordetella bronchiseptica aislada en perros

Abstract

Bordetella bronchiseptica is a bacterium that mostly affects the upper respiratory tract of domestic animals. It remains the leading cause of canine infectious tracheobronchitis, known as kennel cough. This investigation evaluated the presence of Bordetella bronchiseptica and antimicrobial susceptibility in samples of conjunctival exudate, nasal exudates and oropharyngeal mucous membrane, through biochemical tests; 115 samples of dogs were taken (puppies, adults and old dogs), patients should have clinical signs of respiratory disease; the samples were taken in two rescue centers in the city of Cuenca (Clínica Veterinaria Solidaria and Arca Foundation, located in San Sebastian , and “Pichacay” shelter located in Santa Ana). The samples were processed at the microbiology laboratory of the School of Veterinary Medicine, they were incubated for 24 hours in brain heart infusion broth at 37 ° C, then planting in blood agar and MacConkey agar and incubated at 37 ° C for 24 hours, the suspect colonies underwent biochemical tests for identification (gram stain, catalase production, oxidase, nitrates, and urease production, using citrate and malonate and motility test), Finally, antibiotic susceptibility tests were performed using the colonies isolated and identified. Purposive stratified sampling was used, and concluded that the bacterium is present in 9.57% of patients with infectious tracheobronchitis; The sample was taken from three different sites being in a higher percentage in the nasal exudates 72.7%. The statistical test Chi 2 was applied, which showed no correlation between age and the presence of Bordetella bronchiseptica, as well as between the variable sex and presence of Bordetella bronchiseptica. According to sensitivity tests, it was concluded that the Bordetella bronchiseptica strains isolated showed increased sensitivity to antibiotics Criprofloxacina and imipenem with 90.0%, followed by azithromycin and gentamicin with 72.7% and nalidixic acid ranking third with 54.5 % of susceptibility; other antibiotics used for the study show a percentage lower than 50% action