Calibración y validación del equipo milkoscan FT1 para la determinación de parámetros fisicoquímicos en leche cruda en la Industria de Lácteos San Antonio C.A.

Abstract

This thesis arises from the need to use the equipment MilkoScan FT1 for analysis of raw milk, as a measure of quality control. It is a manufactured equipment technology based on FTIR analyzer from FOSS MilkoScan FT120. The study was conducted in San Antonio Dairy Company located in the Industrial Park of the City of Cuenca. The project objective was to calibrate and validate the equipment MilkoScan FT1 in analyzes of fat, total solids, nonfat solids and specific gravity at 20 ° C in raw milk. For calibration were used 80 raw milk samples. For the regression was used the partial least squares model, obtaining a correlation coefficient (r) of 0.999 and a standard error of prediction (EEP) to fat 0.009; a r of 0.996 and 0.147 of EEP to total solids; a r of 0.990 and 0.061 of EEP to non-fat solids; r of 0.993 and 0.633 EEP to relative density at 20 ° C. In the validation were analyzed 80 raw milk samples. The validation showed that the analytical method used was linear, precise, accurate, sensitive, robust in the concentration range studied. Both the validation process and the development of analytical procedures were performed in order to meet the technical requirements specified in ISO / IEC 17025 accreditation for Food Science Laboratory.