Cipriani Ávila, Eva IsabelCapparelli, Mariana V.Jara Negrete, Eliza NuitAlvarado Martínez, Andrés OmarCisneros Ramos, Juan FernandoTripaldi, PiercosimoPerez Gonzalez, Bolivar AndresPinos Vélez, Verónica Patricia2021-11-052021-11-0520212673-4117http://dspace.ucuenca.edu.ec/handle/123456789/37279https://www.mdpi.com/2673-4117/2/3/20Among emerging pollutants, endocrine disruptors such as estradiol are of most concern. Conventional water treatment technologies are not capable of removing this compound from water. This study aims to assess a method that combines physicochemical and biological strategies to eliminate estradiol even when there are other compounds present in the water matrix. Na-montmorillonite, Ca-montmorillonite and zeolite were used to remove estradiol in a medium with sulfamethoxazole, triclosan, and nicotine using a Plackett–Burman experimental design; each treatment was followed by biological filtration with Daphnia magna. Results showed between 40 to 92% estradiol adsorption in clays; no other compounds present in the mixture were adsorbed. The most significant factors for estradiol adsorption were the presence of nicotine and triclosan which favored the adsorption, the use of Ca-montmorillonite, Zeolite, and time did not favor the adsorption of estradiol. After the physicochemical treatment, Daphnia magna was able to remove between 0–93% of the remaining estradiol. The combination of adsorption and biological filtration in optimal conditions allowed the removal of 98% of the initial estradiol concentration.es-ESNatural claysEmerging contaminantsZeoliteBentoniteWastewater treatmentAdsorptionDaphnia magnaARTÍCULO10.3390/eng2030020