Cornejo Franco, Juan FranciscoMedina Salguero, AndrésFlores Flor, FranciscoChica Martínez, Eduardo JoséGrinstead, SamuelMollov, DimitreQuito Ávila, Diego Fernando2021-01-072021-01-0720200929-1873, e 1573-8469https://www.scopus.com/record/display.uri?eid=2-s2.0-85086655122&origin=resultslist&sort=plf-f&src=s&st1=EXPLORING+THE+VIROME+OF+VASCONCELLEA+X+HEILBORNII%3a+THE+FIRST+STEP+TOWARDS+A+SUSTAINABLE+PRODUCTION+PROGRAM+FOR+BABACO+IN+ECUADOR&sid=523339cb06631a851b0059ec86bd67aa&sot=b&sdt=b&sl=143&s=TITLE-ABS-KEY%28EXPLORING+THE+VIROME+OF+VASCONCELLEA+X+HEILBORNII%3a+THE+FIRST+STEP+TOWARDS+A+SUSTAINABLE+PRODUCTION+PROGRAM+FOR+BABACO+IN+ECUADOR%29&relpos=0&citeCnt=5&searchTerm=&featureToggles=FEATURE_NEW_DOC_DETAILS_EXPORT:1,FEATURE_EXPORT_REDESIGN:0The virome of babaco (Vasconcellea x heilbornii) —a non-traditional fruit crop native to Ecuador— was investigated by high-throughput sequencing (HTS) on plants obtained from a commercial nursery. Six virus-like sequences were detected, including the full length of papaya ringspot virus (PRSV) and an RNA-dependent-RNA-polymerase (RdRp) sequence with homology to papaya virus Q. Three RNA sequences were found with homology, respectively, to apple latent spherical virus (genus Cheravirus, 71% nt identity), cherry leaf roll virus (genus Nepovirus, 54% nt identity) and Citrullus lanatus cryptic virus (genus Deltapartitivirus, 66% nt identity); whereas a DNA pararetrovirus-like sequence with homology to citrus endogenous pararetrovirus (58% nt identity) was also detected. RT-PCR-based virus surveys on a total of 284 samples collected from three provinces revealed that the partitivirus- and pararetrovirus-like sequences were present in 100% of tested plants; whereas the other virus sequences were detected in up to 68% of plants and were associated with different symptoms. This work provides information on the occurrence and prevalence of PRSV and five additional virus-like sequences in babaco, a vegetatively propagated crop, supporting the need for a virus-free certification program.es-ESVasconcellea x heilborniiVirusBabacoPapayaEcuadorARTÍCULO10.1007/s10658-020-02037-2