A modified lateral flow assay, using serum, for the rapid identification of human and bovine cysticercosis in the absence of false positives.

Rojas, Glenda C.Sastre, PatriciaCortéz, María MilagrosHarrison, LeslieParkhouse, MichaelCarpio Rodas, Luis ArturoCampoverde Cisneros, Manuel Alfredo2023-07-122023-07-1220190035-9203http://dspace.ucuenca.edu.ec/handle/123456789/42383https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85061121773&origin=inwardBackground Previously we reported the use of a monoclonal antibody–based (HP10) antigen (Ag) detection lateral flow assay (LFA) for the diagnosis of extraparenchymal neurocysticercosis (EP-NCC). The assay performed well when used with cerebrospinal fluid (CSF) samples but not with their paired serum samples, due to false-positive reactions in some known negative control cases. Methods Our novel modification involves pretreatment of serum samples using a combination of sodium deoxycholate and dithiothreitol. Results The modification overcomes the problem of false positives when using negative serum samples from clinically characterized cases of EP-NCC and bovine cysticercosis. In general, there was good agreement between HP10 Ag enzyme-linked immunosorbent assay (ELISA) and the HP10 Ag-LFA, but the HP10 Ag-ELISA was …Background Previously we reported the use of a monoclonal antibody–based (HP10) antigen (Ag) detection lateral flow assay (LFA) for the diagnosis of extraparenchymal neurocysticercosis (EP-NCC). The assay performed well when used with cerebrospinal fluid (CSF) samples but not with their paired serum samples, due to false-positive reactions in some known negative control cases. Methods Our novel modification involves pretreatment of serum samples using a combination of sodium deoxycholate and dithiothreitol. Results The modification overcomes the problem of false positives when using negative serum samples from clinically characterized cases of EP-NCC and bovine cysticercosis. In general, there was good agreement between HP10 Ag enzyme-linked immunosorbent assay (ELISA) and the HP10 Ag-LFA, but the HP10 Ag-ELISA was …es-ESBovine cysticercosisNeurocysticercosis (NCC)HP10 antigen LFAField diagnosisHP10 antigen ELISAA modified lateral flow assay, using serum, for the rapid identification of human and bovine cysticercosis in the absence of false positives.ARTÍCULO10.1093/trstmh/try116.