Astiz, SusanaAyala Guanga, Luis EduardoDutan Sanango, Jorge BolivarSamaniego Campoverde, Jorge XavierJacome Aucay, Andres SantiagoGaray Pena, Gabriela SofiaRodas Carpio, Ermes Ramiro2024-03-042024-03-0420230936-6768http://dspace.ucuenca.edu.ec/handle/123456789/44016https://onlinelibrary.wiley.com/doi/epdf/10.1111/rda.14488The objective of this research was to investigate the diagnostic accuracy of post- mortem ultrasound in antral follicle count (AFC) determination and compare it with visual AFC in grazing crossbred Holstein cows, at high altitude in Ecuador. Pre- mortem blood from 80 cows was collected, and AFC and ovarian characteristics were ana-lysed post- mortem by ultrasound and visual techniques. AFC counts were stratified as high, medium or low by terciles. Mean AMH concentration in pre- mortem blood was 280.1 ± 15.53 pg/mL. The AFC obtained by visual inspection (26.9 ± 9.49 follicles) was 23.8% higher than by ultrasound (20.5 ± 7.53 follicles) in all ovaries. Body condi-tion score, age and weight of the cattle did not interact with the count technique. In the low AFC group, visual inspection and ultrasound provided similar AFC results. However, in the Medium- and High-AFC groups, AFC by ultrasound was 14.9% lower than AFC by visual inspection. We confirm that ultrasound can be used with great accuracy for AFC >3 mm (close to the resolution limit) in grazing crossbred Holstein cows at high altitude.es-ESAntral follicle countFollicle developmentOestrous cycleOvaryPost-mortemARTÍCULO10.1111/rda.14488