Laboratorio Clínico-Pregrado

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Amilasa pancreática sérica en personas de 23 a 42 años de la ciudad de Cuenca-Ecuador, 2009-2010
(Universidad de Cuenca, 2010-11-11) Polo Peñafiel, César Daniel; Quituisaca Cabrera, Marco Antonio; Cabrera Vicuña, José Antonio; Álvarez Herrera, María Hermelinda
The objective of this project was to determine the levels of serum pancreatic amylase in people who are between 23 to 42 years old of any sex, high and sex who live in Cuenca Ecuador at least for the two last years with the purpose of establishing values of reference according to our reality. This descriptive research was done on 1000 people chosen randomly in the urban parishes of the city. From this amount of people 64.7% are women (647).; with an arithmetic mean of tall of 160 cm.; an arithmetic mean of weight of 64 kilograms. They were asked several questions in order to know their current health condition. They were asked also about their medical history which let us chose healthy people. The blood samples were collected and analyzed under very rigid norms of bio-security at the Clinic Laboratory of the Diagnosis Center of the School of Medicine of the University of Cuenca. A Spectronic Genesys 20, spectrophotometer was used. The reagents were obtained from the chemical store Wienner. The average value of amylase in male people is 77,8 AU /dL and in female people is 71,9 AU /dL. This gives a total average of 74 ± 0,65 AU/dl. We found a significant direct relationship with the Chi square test between the variables sex amylase: (p=0,000); we doesn`t found a significant direct relationship between; amylase with age: (p=0,069); amylase with high: (p=0,265); amylase with weight : (p=0,311). KEY WORDS: Amylases, reference values, urban population, adult, Cuenca – Ecuador.
Anormalidades cromosómicas, mediante citogenética convencional en abortos espontáneos, en pacientes del Hospital Vicente Corral Moscoso. Cuenca. 2016
(Universidad de Cuenca, 2016-11-11) Macas Quishpi, Digna Elizabeth; Peláez Cajas, Diana Elizabeth; Larriva Villarrreal, Diana Katherine; Mejía Chicaiza, Jorge Victoriano
BACKGROUND: The human reproduction is considered as a relatively inefficient process; the spontaneous abortions occur in 10-15% of all clinically recognized pregnancies. Chromosomal abnormalities are one of the main causes for such loss occurs. To this postponement of motherhood which directly influences a high incidence of chromosomal abnormalities adds. In addition, statistics of chromosomal aberrations in spontaneous abortions amounted to gestational age and if the woman has a history of abortions. (1) (2) OBJECTIVE: To determine by conventional cytogenetics chromosomal abnormalities in spontaneous abortions in patients Hospital Vicente Corral Moscoso in 2016. METHODS: An observational, analytical cross-sectional study was conducted in a six-month period 100 samples were collected abortion. As laboratory method analysis abortion tissues used by conventional cytogenetics; plus the patient data were obtained through a form, they were tabulated and associated with the result of the karyotype. The data obtained were analyzed in the SPSS version 1.8 software in Spanish and Excel 2010. RESULTS AND CONCLUSIONS: We obtained 59 karyotypes of processed samples and 33.9% had chromosomal abnormalities, the most common abnormalities were aneuploidy by 50%. No statistically significant association with the associated variables found. The results of the study were provided to each of the patients it was useful for counseling
Bacterias causantes de sepsis neonatal y su perfil de susceptibilidad en el Hospital Vicente Corral Moscoso, 2015 - 2018
(Universidad de Cuenca, 2020-03-04) Cabrera Plaza, Aracely Leslie; Cáceres Palacios, Jennifer Johanna; Agreda Orellana, Ivanna Solmayra
INTRODUCTION: Neonatal sepsis is responsible for a high percentage of the worldwide mortality rate. It’s defined as an abnormal immune response that is used to fight off a systematic invasion of microorganisms such as fungus, bacteria, and viruses that are in the bloodstream during the first 28 days of life. The symptomatology is nonspecific and the risk factors are maternal – neonatal. GENERAL OBJECTIVE: To determine the bacteria that causes neonatal sepsis and their susceptibility profile in the Vicente Corral Moscoso Hospital, 2015 - 2018. METHODOLOGY: A descriptive-retrospective study was done with universal and a sample of 1.200 infants were performed. Blood culture results and associated factors such as sex, gestational age and days of hospitalization were collected. The data was analyzed and tabulated in the SPSS version 22 and Microsoft Excel 2016 programs. RESULTS: Of 122 diagnoses of neonatal sepsis, 27,1% presented positive blood culture. The most affected population were women with 54,5%, preterm infants in 66,7% and the hospital stay was ≥8 days at 100%. The main causative agents were: Staphylococcus epidermidis 42,5% and Staphylococcus hominis 12,2%, both producers of betalactamases and resistant methicillin. The most pathogenic bacterium was Klebsiella pneumoniae 3,3%, producer of extended spectrum betalactamases and carbapenemases. CONCLUSION: Neonatal sepsis mainly affected women and preterm infants. The bacteria most affected were Staphylococcus spp. negative coagulase and were found resistance markers: BLEE, BLACT, MRS, MLSb and carbapenemases production.
Bilirrubinas séricas en personas de 23 a 42 años de la ciudad de Cuenca-Ecuador, 2009-2010
(Universidad de Cuenca, 2010-11-11) Peñaloza Ordóñez, Mayra Isabel; Vizhñay Fajardo, Jessica Maricela; Cabrera Vicuña, José Antonio; Alvarez Herrera, María Hermelinda; Cabrera Vicuña, José Antonio
Was evaluated serum levels of bilirubin in healthy people 23 to 42 years in the urban parishes of the city of Cuenca, who helped apply a survey to assess their health status and thus become part of our study. We obtained blood samples from fasting, they were processed in the laboratory of the Diagnostic Center for identification, we used the spectrophotometer Genesis 20, and the commercial reagent Wiener Bilirubin results relate to the variables of age, sex, height and weight. We used SPSS 15.0 where the average value of total bilirubin was: 0.8 ± 0.009 mg / dl, a standard deviation of 0.3 mg / dl, the minimum value of 0.2 mg / dl and the maximum 1.5 mg / dl. Direct bilirrubin was the average value: 0.15±0.002 mg/dl, a standard deviation of 0.06 mg/dl, the mínimum value of 0.01 mg/dl and the máximum value of 0.30 mg/dl; and indirect bilirubin average value: 0.50 ± 0.007 mg / dl, a standard deviation of 0.23 mg / dl, the minimum value of 0.00 mg / dl and the máximum value of 1.00mg/dl. There is no significant correlation between total bilirubin, age (p = 0.243), with sex (p = 0.212), height (p = 0.721), weight (p = 0.865) were not significant direct bilirubin relation ships - age(p = 0.148), with sex (p = 0.264); size (p = 0.909), weight (p = 0.215) and also between indirect bilirubin – size (p = 0.217), weight (p = 0.361), being significantly related indirect bilirubin - sex (p = 0.003), age (p = 0.046). Keywords: bilirubin, reference value, technical and laboratory procedures, healthy, Cuenca-Ecuador.
Calcio sérico en personas de 23 a 42 años de la ciudad de Cuenca-Ecuador. 2009-2010
(Universidad de Cuenca, 2011-11-11) Vivanco Tinoco, América Maritza; Seminario Vintimilla, Laura Elizabeth; Cabrera Vicuña, José Antonio; Alvarez Herrera, María Hermelinda
This study aimed to determine the values of Calcium in persons 23 to 42 years old of different sex, height and weight, in order to establish values reference. The study was conducted in 1000 randomly selected people in the urban parishes of the city of Cuenca; who were applied a questionnaire covering their current health status, the personal and family medical history, which allowed selection of healthy individuals. Research was conducted in the Clinical Laboratory of the Diagnostic Center at the Medical School of the University of Cuenca. A Spectronic Genesys 20 brand spectrophotometer and a reactive substance from the Wienner business were used for this research. Of the research subjects, 35% were men and 65% women. The majority, 34%, of subjects were between the ages of 23 and 26, 23,1% of subjects were between the ages of 39 and 42 with a total of 57,1 %,73.3% of subjects were between 151 and 170 cm tall, with an average height of 160 cm. 67% of subjects weighed between 51 and 70 Kg with an average weight of 63, 4 Kg. The obtained results were as follows: the average calcium level was 9,4 ± 0,0204 mg/dl with the84,5 % , with an average for men of 9,5 mg/dl and an average for women of 9,3 mg/dl. Broken down by ages, subjects between the ages of 23 and 26 had an average calcium level of 9,4 mg/dl. For subjects between the ages of 27 and 30 the average calcium level was 9,3 mg/dl, 9,3 mg/dl for subjects between the ages of 31 and 34, 9,4 mg/dl for subjects between the ages of 35 and 38, and 9,5 mg/dl for subjects between the ages of 39 and 42, with a resulting total Chi-square value of 0,164. Key words: Calcium, value of reference, urban population, adult, Cuenca Ecuador.
Calcio sérico en personas de 23 a 42 años de la Ciudad de Cuenca-Ecuador. 2009-2010
(Universidad de Cuenca, 2010-11-11) Vivanco Tinoco, América Maritza; Seminario Vintimilla, Laura Elizabeth; Cabrera Vicuña, José Antonio; Álvarez Herrera, María Hermelinda; Cabrera Vicuña, José Antonio
This study aimed to determine the values of Calcium in persons 23 to 42 years old of different sex, height and weight, in order to establish values reference. The study was conducted in 1000 randomly selected people in the urban parishes of the city of Cuenca; who were applied a questionnaire covering their current health status, the personal and family medical history, which allowed selection of healthy individuals. Research was conducted in the Clinical Laboratory of the Diagnostic Center at the Medical School of the University of Cuenca. A Spectronic Genesys 20 brand spectrophotometer and a reactive substance from the Wienner business were used for this research. Of the research subjects, 35% were men and 65% women. The majority, 34%, of subjects were between the ages of 23 and 26, 23,1% of subjects were between the ages of 39 and 42 with a total of 57,1 %,73.3% of subjects were between 151 and 170 cm tall, with an average height of 160 cm. 67% of subjects weighed between 51 and 70 Kg with an average weight of 63, 4 Kg. The obtained results were as follows: the average calcium level was 9,4 ± 0,0204 mg/dl with the84,5 % , with an average for men of 9,5 mg/dl and an average for women of 9,3 mg/dl. Broken down by ages, subjects between the ages of 23 and 26 had an average calcium level of 9,4 mg/dl. For subjects between the ages of 27 and 30 the average calcium level was 9,3 mg/dl, 9,3 mg/dl for subjects between the ages of 31 and 34, 9,4 mg/dl for subjects between the ages of 35 and 38, and 9,5 mg/dl for subjects between the ages of 39 and 42, with a resulting total Chi-square value of 0,164. Key words: Calcium, value of reference, urban population, adult, Cuenca Ecuador.
Calcio sérico por fotocolorimetría en mujeres de 45 a 55 años de la Ciudad de Cuenca 2013
(Universidad de Cuenca, 2013-11-11) Samaniego Espinosa, Sandra Tamara; Fernández Iñiguez, Patricia Guisella; Cárdenas Carrera, Jenny Carola; Cabrera Vicuña, José Antonio
The calcium, essential for the human body, is kept at levels from 8.5 to 10.5 mg / dl. Higher levels (hypercalcemia) are detected in the menopause, when the rate of reabsorption increases regarding the reduction of ovarian estrogen production and, consequently, more calcium is needed to manage the reduction in the efficiency of the use of mineral obtained from food. It was made an observational and descriptive study, which purpose was to determine the serum calcium using photocolorimetry in 45-55 years old women. According to the aim, 201 blood samples from patients belonging to urban area parishes of the city of Cuenca were analyzed, in order to identify the relationship between hypercalcemia and hormonal alteration these women. The processing of the samples was performed at the Laboratory of the” Vicente Corral Moscoso” Hospital, following biosafety standards and quality control. Next, the data was entered to SPSS and Excel software, getting these results: The 50.2% of women surveyed are over 50 years old. 56.7% of these women have a rate of calcium between 9 and 10.9 mg / dl, while 33.3% have values higher than 11 mg / dl relating it to a study carried out in Costa Rica with outpatient population in 45 healthy adult women randomly selected, without apparent disorders of calcium metabolism, in which was obtained serum calcium between 8.6 - 10.3 mg / dl.
Caracterización bacteriana en flora intestinal y pruebas de resistencia antibiótica en 500 escolares del sector urbano de Cuenca, año 2009
(Universidad de Cuenca, 2011-11-11) Jimbo Méndez, Janeth María; Saquicela Quezada, Sandra Tarcila; Galindo Banegas, Telmo Benjamín; Alvarez Serrano, Marlene Elizabeth
Objective: Bacterial characterization in Intestinal Flora and Tests of Antibiotic Resistance in 500 scholars of the Urban Sector of Cuenca, Year 2009 .-Materials and Methods. With an Observational Descriptive design, 500 samples of fecal grounds were gathered of school and they were processed in the laboratory of microbiology of the Ability of Sciences you prescribe, during the period September of the 2009. - Results. -Of the 500 scholars 279 were women and 221 men, the percentage distribution for ages was: 5-7 years (33.6%), 8-10 years (44.4%) and of 11-13 years (22.0%). The carried out bacterial isolations showed to Escherichia coli in 80%, Enterobacter 13%, Klebsiella 5.8%, Proteus 0.8% and Pseudomona 0.4%. - It was determined that all the bacterias turned out to be sensitive in a high percentage to Imipenem 100%, Cloranfenicol 98.4%, Ciprofloxacina 97.5%, Cefotaxime 95.5%, Cefurexime 93.3%, and Ceftriaxona 90.2%. Escherichia coli presents resistance to Trimetoprinsulfa with 20%, Gentamicina 23.5% and Ampicilina+ Sulbactam 25.3%; as for the Enterobacter it was it for Gentamicina 15.4%, Ampicilina+Sulbactam 18.5%, in what concerns Klebsiella 24.1% point for Gentamicina and Ampicilina+ Sulbactam and Trimetoprinsulfa 27.6%. Proteus and Pseudomona didn't present resistance some. Conclusions. - Escherichia coli was the bacteria of more frequency contrary to Klebsiella, Enterobacter, Proteus and Pseudomona, the bacterial resistance was comparatively low.AU
Caracterización y resistencia de escherichia Coli a los antimicrobianos en los hospitales Vicente Corral Moscoso y José Carrasco Arteaga, Cuenca, 2008-2009
(Universidad de Cuenca, 2011-11-11) Alvarado Sánchez, Sebastián Stalyn; Guzmán Rodas, Fernanda Elizabeth; Saquipay Pañi, Wilson Roberto; Galindo Banegas, Telmo Benjamín; Álvarez Serrano, Marlene Elizabeth
Objective. To characterize and determine the strength of Escherichia coli in primary cultures in Vicente Corral and Jose Carrasco hospitals of Cuenca city. Materials and method. A descriptive design information was collected 1000 primary cultures in external consultation and hospitalization of both institutions of health in period January to May 2009. Results. In the 1000 primary cultures 789 (79%) were at the Hospital Vicente Corral and 211 (21%) in the Hospital José Carrasco identified 352 (35.2%) positive cultures: Escherichia coli, in both hospitals the highest percentage of samples came from the outpatient clinic, the bacterium Escherichia coli showed increased resistance to TMP + SMX association in 50% of crops, there is resistance to other antibacterial agents: cefepime, nitrofurantoin, ceftriaxone, ceftazidime, ciprofloxacin, imepenem, gentamicin and sulbactam, ranging from 12.8% to 22.2%, respectively. Collection characterized the distribution of frequencies as: age, sex and origin of material for cultivation and antibiogram. . Conclusions. Bacterial resistance patterns are changing and differ between health houses. Bacterial behaviour seems to be determined by abuse of antibiotics. Key words: Culture media, antibiogram, bacteria, bacterial drug resistance, Escherichia coli.
Caracterización y resistencia de proteus, pseudomona, klebsiella y enterobacter en 1000 cultivos primarios en pacientes de los hospitales Vicente Corral Moscoso y José Carrasco Arteaga, Cuenca, 2008-2009
(Universidad de Cuenca, 2010-11-11) Sánchez Alvarado, David Fernando; Carrasco Contreras, Maribel Fabiola; Campoverde Ochoa, Mariela Soledad; Galindo Banegas, Telmo Benjamín; Alvarez Serrano, Marlene Elizabeth
Objective. Characterize the sensitivity and resistance of Proteus, Pseudomonas, Enterobacter, Klebsiella isolated in crops in the Vicente Corral and Jose Carrasco hospitals of Cuenca city. Materials and Methods. With a cross sectional study was collected information 1000 primary cultures in the outpatient and hospitalization of both houses of health from 2008 to 2009. Results. In the 1000 primary cultivations 789 (79%) picked up in the Hospital Vicente Corral and 211 (21%) in the Hospital José Carrasco 137 were identified (13,7%) positive cultivations for: Klebsiella spp (5,5%), Enterobacter spp (4,9%), Proteus spp (1,7%) and Pseudomona spp (1,6%). In both hospitals the biggest percentage was of the Service of External Consultation. Pseudomona spp went more resistant to Cefepima (53,4%). Gentamicina (50%) and Ceftriaxona (50%); Klebsiella spp to Ampiciline Sulbactam (52,8%) and Gentamicina (40%); Enterobacter spp to Gentamicina (65,4%), and Proteus spp to Ciprofloxacina (41,2%) and Sulbactam (35,3%). The summary characterizes the variables of interest like age, sex and bacterial resistance in patient as well as the hospital areas where were assisted and collection the sample for cultivation and antibiograma. Technique was used settled down in cultivation microbiology (primary, secondary), you prove biochemical of identification and antibiograma of Kirby Bawer, validated internationally. Conclusions: Sensitivity and bacterial resistance patterns are very changing and differ markedly between health centres. Bacterial behavior seems to be determined by the abuse of antibiotics. KEY WORDS: bacteria - characterization - proteus, pseudopretty, klebsiella, to enterobacter - bacterial farmacoresistencia - technical and procedures of laboratory - patient, hospitals public, Cuenca - Ecuador
Conocimientos, actitudes y prácticas sobre papiloma virus humano en estudiantes de la Escuela de Tecnología Médica-Universidad de Cuenca, 2016
(Universidad de Cuenca, 2017-11-11) Sinchi Tasambay, Hilda Maruja; Yupa Alvarez, Valeria Jaqueline; Macero Méndez, Reina María; Campoverde Cisneros, Manuel Alfredo
Background: It is very important to find out the knowledge they have the / os students with respect to Human Papillomavirus because adolescents to have an active sex life at an early age are more likely to contract the infection.1 Objective: To identify knowledge, attitudes and practices on human papilloma virus in students from the School of Medical Technology at the University of Cuenca 2016. Methodology: descriptive study, which allowed knowing the knowledge, attitudes and practices on human papilloma virus. The universe was 660 students of the School of Medical Technology; the sample was made up 243 students which were obtained by applying a standard deviation of the population of 0.5, a confidence level of 95% and an acceptable limit of sampling error, 5% (0.05). Prior to the development of research students gave written informed consent, surveys was applied with information regarding the study variables: age, sex, marital status, race, age of first sexual intercourse, sexual partners, knowledge, attitudes and practices on HPV. Scorekeeping for the relevant forms were used and processed for tabulation in Microsoft Word, Microsoft Excel and SPSS. Results: 65.8% mentioned receiving information about HPV, recognize that affects both men and women 69.1%. 28% do not know that complications can bring HPV infection; 37.9% indicated having active sex life
Conocimientos, actitudes y prácticas sobre parasitósis intestinal en adultos de las parroquias rurales del Cantón Cuenca-Ecuador. Intervención educativa. 2013-2014
(Universidad de Cuenca, 2014-11-11) Gualán Cabrera, Luz María; Loja Lema, María Mercedes; Orellana Paredes, Patricia Anabel; Cabrera Vicuña, José Antonio
This descriptive and educational research conducted to 202 subjects. The subjects were residents of the suburban areas of Cuenca City. The subjects were selected randomly at Sayausí, Sinincay, Baños y Chiquintad. Subjects were evaluated in their Knowledge, attitudes and practice (CAPs) of intestinal parasites. The evaluation was conducted by using a pre-test and a post-test. The Educational program used parasites general scientific concepts applied in Epidemiology, therapeutics, clinical and preventive areas. During the pre-test, 67% of the subjects demonstrated general knowledge of parasites, 70.8% of the subjects indicated that parasites could be physically observed. 31.2% of the subjects indicated that eating sweets is the source of transmitting parasites. 11.9 % of the subjects indicate that parasites are due to poor hygiene, considering that untreated tap water may help parasites to spread, also the subjects stated that flies are the vector to transmit parasites. 51% of the subjects indicated that parasites are located in the intestines only. 62% of the subjects indicated that parasites could be host in the heart, liver and brain, etc. 64% of the subject indicated no knowledge of physical characteristics of parasites. If having domestic animal is considered a source of spreading parasites. 70.56% of the subjects stated that dogs transmitted parasites. 23.25% of the subjects indicated that domestic births are source of parasites. Regarding to parasites treatment: 54% of the subjects indicated that they prefer to use anti-parasitic medicine available in the local pharmacies, 17.3% of the subjects used home made medicine when they suspect parasitic disease; and 10.9% goes to the doctor. After an educational intervention and using the post-test to evaluated subjects knowledge; the study find out that the knowledge, attitude and practice of subjects concerning parasites improved considerably, All the percentage indicators increase in general, in some cases reaching 100%.
Control de diabetes mellitus tipo 2 mediante valoración de hemoglobina glicosilada A1c e intervención educativa en pacientes del departamento de endocrinología del Hospital Vicente Corral Moscoso Cuenca-Ecuador 2011
(Universidad de Cuenca, 2011-11-11) López Bautista, Lorena Elizabeth; Maldonado Palacios, Ana María; Sarmiento Webster, María Belén; Alvarez Herrera, María Hermelinda; Cabrera Vicuña, José Antonio
We determined the values of glycated hemoglobin A1c and blood glucose in patients with Type 2 diabetes mellitus Department of Endocrinology, Hospital Vicente Corral Moscoso of the city of Cuenca, Ecuador in 2011. This educational intervention descriptive study was conducted in the Clinical Laboratory Diagnostic Centre, Faculty of Medical Sciences. Universe was taken as all people with type 2 diabetes mellitus Department of Endocrinology, Hospital Vicente Corral Moscoso, with a sample of 150 patients who had to answer a survey and found at baseline. Universe was taken as all people with type 2 diabetes mellitus Department of Endocrinology, Hospital Vicente Corral Moscoso, with a sample of 150 patients who had to answer a survey and found at baseline. HbA1c values was associated with the variables: age, sex, education level, physical activity, time of suffering and fasting glucose. We obtained the following results: average value of fasting blood glucose of 135 ± 2.8 mg / dl, standard deviation 34.6 mg / dl, maximum value 317.6 mg / dl, minimum value 98.9 mg / dl , median 123.5 mg / dl, fashion 102.0 mg / dl and variance 1195.1 mg / dl, mean value of glycosylated hemoglobin A1c is 8.3 ± 0.1%, 1.6% standard deviation, value 14.5% maximum, minimum value 5.4%, median 8.0%, 7.8% fashion and variance 2.6%. By relating the results obtained with the variables found that there is statistical significance between glycosylated hemoglobin and time of illness (p=0.000), glycosylated hemoglobin and fasting glucose (p=0.000) on the other hand in the relationship between glycosylated hemoglobin and sex (p=0.442), glycosylated hemoglobin and age (p = 0.376), and glycated hemoglobin level of education (p = 0.468) no statistical significance. There were three educational interventions in the Auditorium of the Hospital Vicente Corral Moscoso, we applied a pre-test and post-test to deepen understanding of the issues and encouraged patients to adequately control their disease to achieve better lifestyle. We conclude that the realization of an educational intervention thesis involves research group directly with the community, allowing health staff awareness and know the reality of the research population. DeCS: Diabetes Mellitus Type 2 – epidemiology; Hemoglobin A Glycosylated; Blood Glucose - Analysis; Health Education – Methods; Health Knowledge, Attitudes, Practice; Patients; Hospital Regional Vicente Corral Moscoso; Cuenca-Ecuador.
Correlación entre glucosa basal y hemoglobina glucosilada en el adulto mayor en el cantón Cuenca, 2015
(Universidad de Cuenca, 2016-11-11) Ulloa González, Mery Patricia; Velásquez Segarra, Katherine Alexandra; Macero Méndez, Reina María; Encalada Torres, Lorena Esperanza
INTRODUCTION: The metabolic syndrome and diabetes among elderly people are caused by alterations in the metabolism of glucose. The determination of glycosylated Hemoglobin has been an exact indicator of glycaemia among individuals for the last three months allowing to have a proof of their health condition. OBJECTIVE: To establish the correlation between the basal glucose and the glycosylated hemoglobin and its association with the metabolic syndrome among elderly people in the city of Cuenca. METHODOLOGY: It is a descriptive research, the participants were 126 elderly people. For this study, the automatic calculator EPI INFO was used. A group of participants with metabolic syndrome met to the Adult Treatment Panel (APT III) criterion. A survey was applied to collect information and blood samples were taken to determine basal glucose and glycosylated Hemoglobin. The gathered data was processed through the SPSS 20.0 version, Excel, and MedLab programs. The values were classified according to age and sex frequency and the relation to the metabolic syndrome. RESULTS: 126 patients between 65 and 96 years old were analyzed, being female elders more frequent, with a 65.1%. The population with metabolic syndrome was 50.8%. The mean of glucose was 87.16% and of HbA1 was 5.65%. After the analysis, a 92% were found in the normal rank of glycaemia and a 92.8% of HbA1. A 4.8% was located in the rank of prediabetes and in the rank of diabetes, a 2.4%. Through Pearson correlation ratio, it was determined a moderated correlation between basal glycaemia and glycosylated hemoglobin: 0,418. It was observed a small relationship, between glucose metabolism alteration and metabolic syndrome due to the 12.5% of patients with this illness showed hyper glycaemia and an 11% HbA1 alteration
Detección de helicobacter pylori en los comerciantes minoristas de la Asociación 9 de Enero, Cuenca 2018
(Universidad de Cuenca, 2019-11-11) Pesántez Lojano, Paola Alexandra; Salinas Cueva, Wendy Gissela; Baculima Tenesaca, José Mauricio
BACKGROUND: For several decades there have been problems that cause the informalization of retailers traders, lack of education, opportunities and socioeconomic situation make them resort to informal trade, becoming a vulnerable group capable of contracting H. pylori. GENERAL OBJECTIVE: To determine the presence of Helicobacter pylori in the retailers traders of the Association January 9, Cuenca 2018. METHODOLOGY: It is a cross-sectional, descriptive and observational investigation for the detection of Helicobacter pylori in the retailers traders of the Association January 9, whose universe is of 110 people, with which it was worked in its entirety. For the execution of the research we are directed towards this population, an informed consent was signed, in addition, the relevant information was obtained through a survey and then the biological samples were obtained. The detection of Helicobacter pylori was performed using the Elisa method in serum and faeces samples, tabulation and analysis of the data were made through the SPSS 22 statistics and the Microsoft Excel program. RESULTS: An incidence of 63,6% was obtained for H. pylori antigen in fecal matter and 68,2% in serum IgG antibodies, the female sex predominated with 60% (feces) and 61,3% (serum), the age between 34 to 51 years was mostly infected. The associated risk factors were type of housing and property, working hours, education, obtaining and meal schedule. CONCLUSIONS: The incidence of H. pylori found in traders is considered significant, taking into account the associated factors, which may predispose to stomach cancer.
Detección de Helicobacter pylori por Microelisa en materias fecales y factores de riesgo en estudiantes secundarios de la Ciudad de Cuenca 2012
(Universidad de Cuenca, 2013-11-11) Bigoni Ordóñez, Gabriele Davide; Aldaz Abril, Ronal Ricardo; Cárdenas Carrera, Jenny Carola; Cabrera Vicuña, José Antonio
This research aims to determine the presence of Helicobacter pylori in 202 students between the ages of 12-19 years of different kind of high school students in the city of Cuenca, Ecuador 2012. 65.3%are femaleand 34.7% male. The study sample was selected at random in the city of Cuenca. For the investigation we applied a questionnaire covering lifestyle, socio-economic, house type, housematerial, infrastructure type, occupation and educational level of parents, habits of cleanliness, food and symptoms at the time of collecting the sample. Feces were examined for identification of Helicobacter pylori by microplate analyzed biosafety standards and quality control, the examination was conducted in the Clinical Laboratory Diagnostic Center of the Faculty of Medicine at the University of Cuenca. We used an ELISA reader brand equipment and reagent Human fecal antigen LINEAR commercial house (CROMATEST), Anti-H. Pylori Ag EIA TEST. Helicobacter pylori was found in 60.4% of high school students; greater in females, age 17-19 years, with health infrastructure and adequate housing conditions, with poor hygiene habits, food, with an average of 8-9 people in the family, with an average monthly income of $ 685
Determinación de microalbuminuria en mujeres embarazadas que acuden al Centro de Salud Carlos Elizalde. Cuenca, 2016
(Universidad de Cuenca, 2017-11-11) Lata Inga, Jenny Alexandra; Neira Agila, Andrea Belén; Cárdenas Carrera, Jenny Carola
BACKGROUND: Microalbumunuria is the excretion of albumin in few amounts in the urine; during pregnancy it can cause serious complications such as pre-eclampsia, which is characterized by increased sarterial pressure in pregnancy. (1, 2) The research was done at the Health Center “Carlos Elizalde” of the Cuenca´s city. GENERAL OBJECTIVE: It was based on the determination of Microalbuminuria in pregnant women who came to the Health Center “Carlos Elizalde” the data were processed by surveys that included data necessary for the research, consent and informed settlement was requested, It collected samples of urine by the patients: the technique used was the Micral-Test and the samples were processed in the laboratory of the Health Center. For data processing SPSS version 22 and Microsoft Excel were used. Participants in the research signed informed consent, where they were provided with necessary information. In case of minors of age, the legal representatives gave their assent, at the same time they applied an (annex 4) survey in which they recorded data of important affiliation related to the variables of study. RESULTS: Of the 164 pregnant women attending the "Carlos Elizalde" Health Center, 12.1% presented positive microalbuminuria between 20 and 100 mg / l, of which 55% were between 15 and 25 years old, 85% were between 20 and 30 weeks of gestation and 15.5% have arterial hypertension
Determinación de la frecuencia de infección por helicobacter pylori y parasitosis en preescolares de 2 a 4 años que acuden a los centros de desarrollo infantil del municipio de la ciudad de Cuenca. Julio, 2015
(Universidad de Cuenca, 2016-11-11) Maldonado Guanga, Gladys Teresa; Buestán Buestán, Doris Elizabeth; Orellana Paucar, Adriana Monserrath; Abril Ulloa, Sandra Victoria
This study was aimed to determine the frequency of Helicobacter pylori and intestinal parasitosis in children from 2 to 4 years attending to daycare centers of Cuenca´s City Hall. Stool samples were collected at daycare centers with colaboration of parents and caretakers. Sample analysis were carried out according to quality control and biosafety standards at the Laboratory of Diagnostic Center of the Faculty of Medical Sciences of the University of Cuenca. Fresh examination was employed for identification of intestinal parasites and immunochromatographic technique for qualitative determination of Helicobacter pylori. Results of this study evidenced a prevalence of 26,1% for Helicobacter pylori infection and 219,3% for intestinal parasitosis in these daycare centers. Entamoeba histolytica was identified as the etiologic agent in 58,8% of parasitosis cases
Determinación de Staphylococcus aureus resistente a la meticilina como colonizante nasal en el personal de salud del Hospital Vicente Corral Moscoso, Cuenca 2018
(Universidad de Cuenca, 2018-11-11) Maza Morocho, Doris Gabriela; Naranjo Naranjo, Lorena Lisseth; Agreda Orellana, Ivanna Solmayra
BACKGROUND: The first outbreak of methicillin – resistant Staphylococcus aureus (MRSA) was recorded in the 70s in the United Kingdom, and this resistance spread throughout the world. The first outbreaks were only related to the intrahospital environments, having as carriers of this microorganism the own health personnel, in the 90s, MRSA strains acquired in the community were diagnosed. GENERAL OBJECTIVE: To establish the incidence of methicillin – resistant Staphylococcus aureus as a nasal colonizer in health professionals of the Vicente Corral Moscoso Hospital corresponding to the period May – October 2018. METHODOLOGY: The present investigation is of analytical and transversal type, 162 health workers were analyzed, samples of nasal swab were obtained for the later determination of S. aureus. The resistance to methicillin was diagnosed through the modified Kirby - Bauer method. The results obtained were examined in the SPSS software version 15.0 for their respective tabulation and analysis. RESULTS: In the year 2018, during the period May - October, an incidence of S. aureus of 20% was obtained, of these isolations 9,4% correspond to MRSA, with the job position of general practitioners, the areas where it was isolated MRSA were the intensive care unit (ICU) and hospitalization with incidents of 3,1% and 6,3% respectively. CONCLUSIONS: The incidence of MRSA found in nasal carriers is considered significant since, it represents a potential risk for the spread of colonization and nosocomial infections at the Hospital Vicente Corral Moscoso.
Determinación del índice homa en transportistas de buses urbanos del cantón Cuenca 2017
(Universidad de Cuenca, 2018-11-11) Soliz Ortega, Yomara Valeria; Valverde Pulla, Jessica Veronica; Cárdenas Carrera, Jenny Carola
BACKGROUND: The HOMA index (homeostatic model for the evaluation of insulin resistance) is a relationship between glucose and insulin that allows the evaluation of the presence of insulin resistance; this is an important factor in developing cardiovascular diseases, metabolic syndrome and diabetes (1). OBJECTIVE: To determine the HOMA index of professional urban drivers in the city of Cuenca. METHODOLOGY: Descriptive study, prospective of a transversal cut, was performer in a universe of 460 professional drivers from urban bus cooperatives of Cuenca. This happened with a full representation of 164 people according to the calculation formula for a finite universe. The people involved in this study signed a consent form, which explain the process to follow and the development of the investigation. These people also filled out a survey form, which allowed us to the results with variables of study. The glucose and insulin tests were performed in the laboratory of the Biochemistry Laboratory of the Medical School Technology of the University of Cuenca. During analysis of the information a program called SPSS was used. RESULTS: From the 164 drivers in this study, 93 showed a high HOMA Index, which determines insulin resistance. It was observed that the conditions of age over 40 years and a working day of more than 8 hours increase the risk of this alteration. 42.6% are sedentary and with a diet high in saturated fat, which is reflected in that 79.6% are overweight and obese, a major factor for the development of insulin resistance and chronic no communicable diseases. CONCLUSION: It was determined that 56.7% of the people studied presented insulin resistance, obesity and sedentary lifestyle being the main factors for their development.

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