Browsing by Author "Valdez Jojoa, Diego Antonio"

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Efecto del extracto del Aloe vera (Aloe barbadensis miller) en la congelabilidad del semen porcino
(2017) Guachún Yanza, Manuel Humberto; Valdez Jojoa, Diego Antonio
The objective of this research was to evaluate the effect of the addition of Aloe vera extract in a cryopreservation medium on the freezing of porcine semen. We used 16 ejaculates of 4 sexually mature boars, from 1 to 3 years, adapted and maintained in a farm in the Guachapala canton, the seminal analyzes and the freezing was carried out in the laboratory of Biotechnologies of the Animal Reproduction of the University of Cuenca; The ejaculates were divided into two aliquots for the treatments: T1.- Freezing of porcine semen in diluent based on sodium citrate and egg yolk; And, T2.- Freezing of porcine semen in diluent based on sodium citrate, egg yolk plus 10% v / v of Aloe Vera in the preparation water. Post-freeze parameters were: progressive individual motility, vitality and membrane integrity (HOS Test). A completely random design was used. Statistical analysis was performed with Student's t test and Mann-Whitney U test. There were no significant differences between the treatments (P> 0.05) on the variables: motility, vitality and abnormalities; However, HOS Test showed higher percentages (P <0.05) in T2 compared to T1 (respectively: 15.54 ± 0.50% and 13.89 ± 0.65%). It is concluded that the addition of the Aloe vera extract to 10% v / v of the media preparation water produced no difference in the parameters of motility, viability; However, decreased the deleterious effects of cryopreservation on the integrity of the plasma membrane of porcine spermatozoa
Eficacia de la radiación ultravioleta en la esterilización del plasma seminal porcino y su comportamiento sobre calidad espermática
(2017) Ordóñez Arias, Wilmer Wilfrido; Valdez Jojoa, Diego Antonio
The objective of the present work was to evaluate the effect of Ultraviolet Short Wave Radiation (UV-C) as a Porcine Seminal Plasma sterilizer and its behavior on sperm quality. Where a random Blocks design was used, consisting of 4 boars from which 4 ejaculated each were divided into 5 aliquots for the raised treatments giving a total of 80 experimental units. The treatments were: A.- no exposure; B.- 5 minutes of exposure; C.- 10 minutes of exposure; D.- 15 minutes of exposure, and E.- 20 minutes of exposure. A 254nm, 12W UV-C radiation sterilization chamber was used. Concentration of CFU / ml, progressive individual motility, vitality, abnormalities and sperm membrane integrity were evaluated. Significant bacterial reduction was obtained from 15 minutes of exposure to ultraviolet radiation type C obtaining 93.8% of sterilized samples. The parameters of sperm vitality, abnormalities and membrane integrity did not show significant differences between treatments (P> 0.05), whereas progressive sperm motility was different (p <0.05), being lower in semen reconstituted with semen plasma With greater exposure to UV-C radiation. It is concluded that UV-C radiation reduces bacterial load significantly as the exposure time to UV-C increases.