Browsing by Author "Rodriguez Coyago, Maria De lourdes"

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Burning mouth syndrome as a manifestation of an unbalanced psycho-neuro immuno-endocrine axis in mentally ill women with intestinal dysbiosis: a literature review
(2022) Bolaños Castillo, Mayra Daniela
Background: Burning mouth syndrome is currently defined as a type of chronic orofacial pain of unknown etiology; however, several publications describe this syndrome as a neurological disorder with multifactorial pathogenesis in which psychogenic, endocrine, and neuropathic factors are involved. The objective of this article is to propose an etiopathogenic model of BMS based on clinical and preclinical evidence published to date. Methods: Through a systematic bibliographic search in 4 scientific databases: PubMed, Science Direct, Embase and the Cochrane library, of articles published in English in the last 20 years. Results: We dare to propose BMS as a trigeminal small-fiber sensory neuropathy influenced by low-grade chronic systemic inflammation, in a scenario of intestinal dysbiosis or psychiatric disorders and exacerbated in peri or postmenopausal women, due to neuroprotective steroid deficiency and greater propensity to psychological disturbances. Conclusion: It is necessary to develop lines of research linked to the taxonomic and functional characterization of the intestinal microbiome in patients with BMS and to compare it with that obtained in mentally ill patients without BMS against a control group, in order, to elucidate its pathogenesis and find new therapeutic targets that allow us to better manage the syndrome, with stable responses over time.
Candida parapsilosis sensu stricto can act as a pathobiont in conditions of oral dysbiosis
(2019) Rodriguez Coyago, Maria De lourdes; Rosa, Alcira Cristina; Jewtuchowicz, Virginia Marta
Recent publications have reported high prevalence of Candida parapsilosis Sensu Stricto in oral cavity niches. Our research group performed a pilot study in 2017 which showed that of the psilosis complex, Candida parapsilosis Sensu Stricto is the species most frequently isolated from oral cavity niches. Under inflammatory conditions, the probability of recovering it is almost four times higher, and it displays high- er biofilm-forming capacity in-vitro, differing significantly from isolates of the same species obtained in conditions of eubiosis. This led us to hypothesize that an oral environment in dysbiosis overregulates vir- ulent genes, promoting a more pathogenic phenotype. Accordingly, we decided to re-evaluate the in-vitro biofilm formation assay using a larger sample, under two nutritional conditions, using colorimetric meth- ods for quantification. In addition, the results obtained were validated by imaging techniques. For both clinical conditions (eubiosis and dysbiosis), high biofilm forming phenotype predominated with both reading methods, and for both nutritional conditions tested. XTT showed significant difference between absorbance values for isolates from buccal dysbiosis and eubiosis (P=0.0025). Candida parapsilosis Sensu Stricto cells that colonize oral cavity niches are basically strong biofilm formers, regardless of in vitro growing conditions. However, the oral cavity in dysbiosis probably promotes virulence in this species due to inheritable epigenetic modifications.
Chronic periodontitis exacerbated by occlusal trauma: report of a case and revision of literature
(2017) Rodriguez Coyago, Maria De lourdes; Iturralde Piedra, Mario Javier; Vega Rivadeneira, Juan Fernando; Pinos Ochoa, Xavier Andres
Occlusal trauma has been associated with periodontal disease 100 years ago, but only observationally. Since the 1930s, the effect of excessive occlusal forces on the periodontium has been evaluated at the pre-clinical level. At first, studies on animal and human autopsy material showed no association between occlusal discrepancies and periodontal destruction. However, in the last 10 years new evidence has emerged that today allows us to establish a relationship between both clinical entities. The latest review on the subject published in 2015, states that at the moment there is a lack of strong evidence to assume a relation of cause/effect between periodontitis and excessive occlusal forces. The objective of this study is to report a clinical case related to a patient with no previous medical history, with an oral diagnosis of an advanced chronic periodontitis, in which the existing occlusal trauma was recognized as a possible accelerating and/or aggravating factor. In addition there is an update of the focused theme.
Deregulation of energy metabolism as a cause and consequence of oncogenic process: review of literature
(2016) Rodriguez Coyago, Maria De lourdes
The reprogramming of energy metabolism is an emerging hallmark of cancer that has not yet been demonstrated in all tumor models, and there are aspects which still not entirely clear. This review enrolled and analyzed data from the last 20 years regarding the proposed theme; and it allowed to conclude that the deregulation of energy metabolism is a key factor in the initiation and progression of tumors, behaving as a cause and consequence.
Distribución y sensibilidad antifúngica del complejo cándida parapsilosis en diferentes nichos de cavidad bucal de una cohorte de pacientes argentinos
(Hospital de Infecciosas Francisco J. Muñiz, Distrito de Diseño CMD, Secretaría e Informes Inés Escalada Congresos y Eventos, 2015) Rodriguez Coyago, Maria De lourdes; Ariza, YY; Facente, Andrea; Magariños, Francisco; Jewtuchowicz, Virginia Marta; Rodriguez Coyago, Maria De lourdes
Distribution and virulence of candida parapsilosis complex oral isolates from immunocompetent subjects with and without gingival-periodontal disease
(2017) Rodriguez Coyago, Maria De lourdes
The Candida parapsilosis complex comprises three genetically related species: Candida parapsilosis sensu stricto, Candida orthop- silosis and Candida metapsilosis. During the last decade, Candida parapsilosis sensu stricto has become the second most commonly isolated yeast from patients with blood stream invasive fungal infections, following Candida albicans. Despite its clinical significance, little is known about its distribution and behavior in oral cavity niches. Given this background, we intended to perform a molecular characterization of 38 isolates of the Candida parapsilosis complex collected from oral cavities of immunocompetent patients with and without gingival-periodontal disease, with the aim of determining its distribution and establishing a correlation with clinical data. Besides, we assessed the virulence of the strains by examining the in vitro biofilm-forming capacity in two different culture media: YPD and RPMI 1640. Being more frequently isolated from oral cavities with pathological conditions, 89.5% of the strains were reconfirmed to be Candida parapsilosis sensu stricto. More than 70% of the Candida parapsilosis sensu stricto strains showed biofilm- forming capacity in both culture media, reflecting a statistically significant association between biofilm-forming capacity and the clinical conditions of the subjects from whom the strains were isolated. Among the three complex species, Candida parapsilosis sensu stricto is a normal commensal in the oral cavity, being more frequently recovered in inflammatory conditions. Gingival-periodontal disease may be a factor triggering overgrowth and virulence of Candida parapsilosis sensu stricto commensal strains.
Ecological imbalance of the oral cavity improves the virulence of candida parapsilosis sensu stricto
(SciTech Central Conferences, 2020) Rodriguez Coyago, Maria De lourdes; Rosa, Alcira Cristina; Jewtuchowicz, Virginia Marta
Candida parapsilosis sensu stricto is the second to third most frequent cause of candidemia. Studies place this yeast as a frequent colonizer of niches of the oral cavity, predominantly in pathological conditions. We hypothesize that a buccal environment in dysbiosis enhances the virulence of C. parapsilosis sensu stricto. Objective: To evaluate at the phenotype and molecular level the production of biofilm in oral isolates of Candida parapsilosis sensu stricto, and correlate the results with the clinical origin (dysbiosis versus eubiosis). Material and methods: The biofilm-forming ability was compared in 50 oral isolates of Candida parapsilosis sensu stricto obtained from patients with and without oral dysbiosis; by quantification of biofilm biomass and metabolic activity. The results were corroborated by optical and confocal fluorescence microscopy, and correlated with the transcriptional activity of CPH2, by RT-qPCR. The data were analyzed by Excel 2010, and InfoStat 2018, with a 95% confidence interval. Results: The metabolic activity in biofilm was significantly higher in oral dysbiosis relative to control (p = 0.0025). Basal expression of CPH2 increased 2.8 times more in oral dysbiosis related to the control condition, and showed no significant differences with pathogenic isolates of this same yeast, derived from onychomycosis lesions. Conclusion: The oral cavity in dysbiosis increases the virulence of C. parapsilosis sensu stricto due to possible changes in epigenetic marks. This finding suggests that the oral cavity in dysbiosis may be an alternative route to the skin in the epidemiology of nosocomial candidemia.
Eficacia clínica de terapia fotodinámica por 5-ALA tópico en el manejo onco-preventivo de lesiones de leucoplasia oral
(2022) Rodriguez Coyago, Maria De lourdes
Oral leukoplakia is the most prevalent malignant disorder of the oral mucosa globally and its management remains a challenge. A systematic review was carried out to determine the clinical efficacy of photodynamic therapy mediated by topical 5- aminolevulinic acid, as an alternative for chemoprevention in the different clinical forms of oral leukoplakia. Materials and Methods An exhaustive search was carried out under MeSH terms for clinical trials published in English in the last 30 years, about the use of photodynamic therapy mediated by topical 5-aminolevulinic acid as a photosensitizer, and under low-intensity laser radiation or LED light as a source of light in different digital bases. Results The systematic review under PRISMA guidance recognized an efficacy of 88.6% for this mode of phototherapy in the management of oral leukoplakia, with a greater effect size in the homogeneous clinical forms with dysplastic changes, regardless of the type of light source. However, the evidence used in this analysis was moderate. Conclusion Based on the results of the present study, we can conclude that 5- aminolevulinic acid topical – mediated photodynamic therapy appears to be a high- performance alternative in the oncopreventive management of oral leucoplakia lesions. However, we recommend running controlled and randomized clinical trials with homogeneous methodologies that allow us to generate a meta-analysis with a high level of evidence.
Eficacia de la bacterioterapia probiótica para la prevención de caries dental: una revisión sistemática de la literatura
(2020) Osorio Ayala, Lesly Damaris
Background: A variety of approaches have been developed for the control of dental caries, a pathology with high incidence and prevalence worldwide. The use of probiotic strains for the modulation of dental biofilm in the prevention of caries has been studied, but the available evidence shows varied methodologies; and the strains tested differ from one study to another. Objective: To analyze through a systematic review of clinical trials, the efficacy of using probiotic strains to prevent dental caries. Methods: A search was made in the scientific bases PubMed, Cochrane and Science Direct, prioritizing randomized double and triple-blind clinical trials from 2010 to 2020, including a total of 20 studies to be analyzed. The selection criteria were consistent with the Preferred Reporting Items for Systematic Reviews protocol. Results: Within the studies, different types of probiotic bacteria were analyzed, dominating Lactobacillus Paracasei. While most clinical trials show a favorable response in terms of a significant reduction of Streptococcus mutans in the oral microbiota, very few studies evaluated salivary pH and sIgA levels. Conclusions: The heterogeneity of the studies analyzed and the multifactorial nature of dental caries do not allow us to ensure that probiotic therapy is completely effective in preventing this pathology. Although probiotic therapy can help by regulating the microbiological factor, there are other determinants that can favor the development of caries and that are barely approached in their relationship with bacteriotherapy. Future studies that homogeneously evaluate the use of probiotics could give us a clearer idea of their effectiveness.
Estrategias de inmunización en caries dental: alcances y limitaciones
(2020) Rodriguez Coyago, Maria De lourdes
Immunization strategies in dental caries: scopes and limitations
(2020) Cantos Tello, Paola Michelle; Torres Tamayo, Gabriela Cecibel; Abad Ortiz, Hugo Alejandro; Rodriguez Coyago, Maria De lourdes
Dental caries is an infectious disease, multifactorial, and considered a global public health problem. Of the cariogenic microbiota, Streptococcus mutans concentrated for years as the main etiological agent of dental caries; leading the scientific community to consider it a target of immunization strategies. However, recent studies discovered a polymicrobial ecosystem with wide variability in dental caries, and S. mutans would represent only a small part of the bacterial community, being found in less than 1% in the different types of carious lesions. Several studies have been carried out that seek to control both the appearance and the advance of cavities, for which, vaccines have been developed in two ways: passive immunization and active immunization. Both types of immunization target antigens expressed on the cell surface of the microorganism. In this study, research on the subject published in the last twenty years is reviewed, in order to establish the current state of this field, and expose its scope and limitations when it comes to meeting the objective of reducing the incidence of caries, which, according to world load studies is still high.
Modulation of oral biofilm and immune response associated to mucosa with probiotic bacteria as a potential approach in the prevention of dental caries: a systematic review
(2020) Osorio Ayala, Lesly Damaris; Buestan Zambrano, Juan Fernando; Yamunaque Vire, Jessica Micaela; Pinos Gavilanes, Maria Paz; Rodriguez Coyago, Maria De lourdes
Background: A variety of approaches have been developed for the control of dental caries, a pathology with high incidence and prevalence worldwide. The use of probiotic strains for the modulation of dental biofilm in the prevention of caries has been studied, but the available evidence shows varied methodologies; and the strains tested differ from one study to another. Objective: To analyse through a systematic review of clinical trials, the efficacy of using probiotic strains to prevent dental caries. Methods: A search was made in the scientific bases PubMed, Cochrane and Science Direct, prioritizing randomized double and triple-blind clinical trials from 2010 to 2020, including a total of 20 studies to be analysed. The selection criteria were consistent with the Preferred Reporting Items for Systematic Reviews protocol. Results: Within the studies, different types of probiotic bacteria were analysed, dominating Lactobacillus Paracasei. While most clinical trials show a favorable response in terms of a significant reduction of Streptococcus mutans in the oral microbiota, very few studies evaluated salivary pH and sIgA levels. Conclusion: The heterogeneity of the studies analysed and the multifactorial nature of dental caries do not allow us to ensure that probiotic therapy is completely effective in preventing this pathology. Although probiotic therapy can help by regulating the microbiological factor, there are other determinants that can favor the development of caries and that are barely approached in their relationship with bacteriotherapy. Future studies that homogeneously evaluate the use of probiotics could give us a clearer idea of their effectiveness.
Molecular characterization of candida parapsilosis species complex in niches of the oral cavity in a cohort of patients from Argentina with different oral and dental clinical manifestations
(2016) Rodriguez Coyago, Maria De lourdes; Jewtuchowicz, Virginia
Candida parapsilosis is considered to be a complex of three species due to genetic heterogeneity. Currently, it is the second most isolated yeast in bloodstream infections and oral cavity sites with C. parapsilosis sensu stricto and C. metapsilosis being the species mostly recovered from oral niches in both healthy and immunecompromised patients respectively. Disparity in antifungal susceptibility has been reported among species of this complex, while no data on distribution and behavior in the oral cavity has been reported in Argentina. Objective: To molecularly characterize a collection of isolates from Argentina, phenotypically identified as C. parapsilosis from different oral niches, in a cohort of patients with different oral and dental clinical conditions. Methodology: This experimental study used 31 yeasts phenotypically recognized as C. parapsilosis and recovered for molecular characterization by end-point PCR using specific primers. The in vitro sensitivity of isolates against azoles, echinocandins, amphotericin and flucytosine was evaluated by automated Vitek2 system and MIC (Minimum Inhibitory Concentration) in line with CLSI (Clinical and Laboratory Standards Institute) M27-A3 guidelines. Results: All isolates, 100% (31/31), were positive for C. parapsilosis sensu stricto species. Of these, 77.4% were recovered in oral inflammatory conditions, and 94.7% of the strains analyzed for antifungal susceptibility profile showed favorable response to all drugs tested. Conclusions: C. parapsilosis sensu strictois a common colonizing of the oral mucosa under pathological conditions, and generally shows favorable response to antifungal agents commonly used in clinical practice, but with a tendency to lower responsiveness to azoles and echinocandins.
Onco-preventive management of oral leukoplakia lesions by topical 5-ALA-mediated photodynamic therapy: a systematic review of clinical trials
(2022) Rodriguez Coyago, Maria De lourdes
Oral leukoplakia is the most prevalent potentially malignant disorder of the oral mucosa globally and its management remains a challenge. Photodynamic therapy is a minimally invasive alternative method in the treatment of premalignant lesions; and the use of the topical 5-ALA photosensitizer plus irradiation with light emitting diode (LED) or laser light has been one of the most recommended approaches for the treatments of oral leukoplakia by photodynamic therapy. The present study was carried out to determine its clinical efficacy as a chemoprevention alternative in the different clinical forms of oral leukoplakia. An exhaustive search of scientific articles published in the last 30 years in English was carried out, using keywords with the MeSH guideline, about the use of topical 5-ALA-mediated photodynamic therapy as a photosensitizer and under low intensity laser radiation or light LED as a source of illumination, in different digital bases. The systematic review under PRISMA guidance recognized an efficacy of 88.6% for this mode of phototherapy in the management of oral leukoplakia, with a greater effect size in the homogeneous clinical forms with dysplastic changes, regardless of the type of light source. However, the evidence used in this analysis was moderate. Based on the results of the present study, we can conclude that topical 5-ALA-mediated photodynamic therapy appears to be a high performance alternative in the oncopreventive management of oral leukoplakia lesions. However, we recommend running controlled and randomized clinical trials with homogeneous methodologies that allow us to generate a met analysis with a high level of evidence.
Optimización de RT-PCR para la evaluación de RNA de la levadura cándida parapsilosis sensu stricto por dos métodos comerciales basados en sistema de purificación por columnas de sílice
(Asociación Argentina de Microbiología, Sociedad Argentina de Bacteriología, 2018) Rosa, Alcira Cristina; Rodriguez Coyago, Maria De lourdes
Oral mucosa as a potential source of candidemia by Candida parapsilosis sensu stricto, under pathological conditions
(2018) Jewtuchowicz, Virginia Marta
Over the past 10 years, there have been increasingly frequent reports of colonization of niches in the oral cavity by Non-Candida Albicans Candida (NCAC) species. Among these species, Candida parapsilosis sensu stricto plays a major part both locally and systemically. Evidence shows that it may be the second most frequent yeast isolate, after Candida albicans, as commensal in the oral cavity and as pathogen in blood cultures of patients diagnosed with fungemia. Recent reports suggest that both its distribution and its virulence may be affected by environmental conditions and may increase in situations of oral dysbiosis. Based on this background, we hypothesize that oral mucosa in pathological condition may be a source of candidemia by Candida parapsilosis sensu stricto, when promoted by the conditions in the host. We reviewed the scientific databases in search of evidence which may support such hypothesis.
Periodontitis determining the onset and progression of Huntington's disease: review of the literature
(2015) Sánchez Temiño, Victoria Emilia
Huntington's disease is a neurodegenerative disorder caused by the expansion of a CAG triplet in the huntingtin gene. It presents with physical, cognitive and psychiatric impairment at different ages in the adult, and has a fatal prognosis. Other than the number of triplet repetitions, there seem to be other factors that explain the onset of this disease at an earlier age. It is well known that neuroinflammation has a key role in neurodegenerative disorders; Huntington's disease is not an exception to that rule. Neuroinflammation exacerbates neuronal damage produced by mutation, by initiating aberrant activation of microglia cell, as well as astrocyte and dendritic cell dysfunction; also compromising the blood-brain barrier and activating the complement cascade. The latter as a direct and indirect effect of the mutation and other stimuli such as chronic infections. In this study, periodontitis is presented as a model of chronic oral infection and a systemic inflammation source. We hypothesize the potential role of periodontitis in Huntington's disease, and the mechanisms by which it contributes to the early onset and progress of the disease. We considered experimental studies, systematic reviews, meta-analyses, published in both Spanish and English, obtained from the PubMed and SciELO databases. There are various mechanisms that generate brain inflammation in these patients; mechanisms of innate immunity being especially prominent. Chronic oral-dental infections, such as periodontal disease, may be an exacerbating factor that adds to the neuroinflammation of Huntington’s disease.
Psychoneuroimmunoendocrine syndrome with impact on the stomatognathic system
(2020) Rodriguez Coyago, Maria De lourdes; Casariego, Zulema Juana
Psychoneuroimmunoendocrinology studies the interaction between the psyche, neural, endocrine and immune function. There are not many works in the literature on the effect of chronic stress on the stomatognathic system. The objective of this article is to present a clinical case with a group of stomatological manifestations. There are, alterations in systemic stress mediators such as cortisol, and local and systemic immunological disorders. Those could be depend on clinical, biochemical and pathological characteristics established in the skin, as antecedents. We propose that the set of its manifestations are the result of an imbalanced psychoneuroimmunoendocrine axis with overactivation of the HPA axis. It could be also favored, by the cyclothymic personality and anxious profile of the patient, determined after a professional psychological study. This work will allow us to consider the “Psychoneuroimmunology” in the etiopathogenesis of disorders such as temporomandibular joint dysfunction, geographic tongue, and cheilitis.
RNA extraction from the yeast candida parapsilosis sensu stricto using two commercial methods based on purification by silica columns
(2018) Rodriguez Coyago, Maria De lourdes; Rosa, Alcira Cristina; Jewtuchowicz, Virginia Marta
Good quality RNA needs to be obtained in order to study gene expression. Different RNA extraction methods have been described, but RNA quality and yield may vary among the different techniques and biological study species. To date, there is no standardized method for extraction and purification of RNA from Candida genus yeasts. The few available papers on the subject apply mainly to filamentous fungi and have produced poor results for extraction techniques based on manual or in-house IVD methods. The aim of this study was therefore to compare two commercial RNA extraction and purification systems using silica columns (Qiagen and Zymo Research) with Candida parapsilosis sensu stricto as model organism. This yeast has been identified in recent papers as the second most frequently isolated Candida species in the oral cavity. In the past decade, it has been the object of increasing medical interest because it is one of the main causes of candidemia in both adults and preterm neonates. In view of this background, we consider the study of Candida parapsilosis sensu stricto transcriptome and its variations according to environmental changes to be a priority. In this experimental study, 19 fungal isolates were processed using Qiagen and 17 isolates using Zymo Research. The results suggest that Qiagen lysis buffer RLT is essential for obtaining better quality RNA product.
RNA extraction from the yeast candida parapsilosis sensu stricto using two commercially available silica column-based purification methods
(2018) Rodriguez Coyago, Maria De lourdes; AC, Rosa; VM, Jewtuchowicz
Good quality ribonucleic acid (RNA) needs to be obtained in order to study gene expression. Different RNA extraction methods have been described, but RNA quality and yield may vary among the different techniques and biological study species. To date, there is no standardized method for extraction and purification of RNA from Candida genus yeasts. The few available papers on the subject apply mainly to filamentous fungi and have produced poor results for extraction techniques based on manual methods. The aim of this study was therefore to compare two commercial RNA extraction and purification systems using silica columns (Qiagen and Zymo Research) with Candida parapsilosis sensu stricto as model organism. This yeast has been identified in recent papers as the second most frequently isolated Candida species in the oral cavity. In the past decade, it has been the object of increasing medical interest because it is one of the main causes of candidemia in both adults and preterm neonates. In view of this background, we consider the study of Candida parapsilosis sensu stricto transcriptome and its variations according to environmental changes to be a priority. In this experimental study, 19 fungal isolates were processed using Qiagen and 17 isolates using Zymo Research. The results suggest that Qiagen lysis buffer RLT is essential for obtaining better quality RNA product.