Browsing by Author "Ortiz Lazo, Christian Israel"

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    Evaluación de la contaminación bacteriana de frascos de Propofol en tres momentos post apertura
    (Universidad de Cuenca, 2024-08-05) Márquez Zhiminaycela, Joseline Andrea; Ortiz Lazo, Christian Israel; Solano León, María Katherine
    Propofol is an intravenous anesthetic drug widely used in veterinary medicine, due to its composition it has a potential risk of contamination; therefore, it requires strict control in its handling; however, little is known about the handling practices regarding the factors that increase the risks of bacterial appearance, this research describes the microbial presence in the content of 20 ml Troypofol (Propofol 1 %) vials. The study was carried out at the veterinary clinic of the University of Cuenca, a group of bottles were stored at room temperature in the drug section of the clinic and another group was placed in the refrigerator located in the office. For sample collection, two different areas of the bottle were chosen, the first sampling site was the rubber stopper; for this, a swab was performed with saline solution (0.9 %), the swabs were stored in Stuart transport medium and taken to the laboratory. The second place from where the sample was obtained was from the content of the bottle, 0.1 ml was aspirated with a 1 ml syringe, the content was placed in red vacutainer tubes without additives for its proper transport. Sample collection was performed at 3 different times (0 hours; 168 hours; 336 hours). The results on day 0 (D0) showed no growth of microorganisms once the bottles were opened, on day 7 (D7) bottle 5 (F5) and bottle 8 (F8) which were stored at room temperature showed 1.000 and 2.000 CFU respectively, on day 14 (D14) all bottles at room temperature were contaminated, samples from bottles 1 (F1) and 4 (F4) which were at refrigeration temperature showed contamination 2000 CFU each; In the identification of the microorganisms, the appearance of Coagulase negative Staphylococcus spp (SCN) was found in all the bottles at room temperature (F5, F6, F7, F8) and in the refrigerated F1, while in F4 (refrigerated) the growth of Bacillus sppBacillus spp. was evidenced. An antibiogram of the microorganisms present in the flasks was performed, where those with SCN showed resistance to Amoxicillin, Ampicillin, Clindamycin and Erythromycin, while the Bacillus sppBacillus spp. isolated from F4 showed resistance to Ciprofloxacin; in the results of the drug rubber stopper there was a complete absence of bacteria