Browsing by Author "Gonzalez Bulnes, Antonio"

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A cross-sectional study of obesity effects on the metabolomic profile of a leptin-resistant swine model
(2020) Sanz Fernández, María Victoria; Torres Rovira, Laura; Pesántez Pacheco, José Luis; Garcia Contreras, Consolacion; Astiz, Susana; Gonzalez Bulnes, Antonio
Identifying metabolite signatures associated with obesity and related diseases might represent a valuable preventive and therapeutic tool to predict subjects at risk, establish an accurate prognosis, and monitor treatment success. The current cross-sectional study is aimed to evaluate the metabolite profile of diet-induced obesity in a porcine model of leptin resistance. Six Iberian female pigs prone to develop obesity (OB) were ad libitum fed a fat-enriched diet (HFD) for 82 days. Five lean Iberian sows (CON) in a maintenance diet served as controls. At the end of the dietary treatments, all animals were sacrificed, and plasma, liver, and muscle samples were immediately collected for nuclear magnetic resonance analysis. In plasma, signals corresponding to betaine, glycerophosphocholine/phosphocholine, glycine, and glutamate were decreased; and the valine signal was increased in OB sows compared to controls. Similarly, the betaine signal was decreased in the liver. No differences were detected in muscle. The observed metabolite changes suggest alterations in branched chain amino-acid metabolism and the methionine-homocysteine cycle, which have been previously associated with obesity-related diseases and type 2 diabetes in human observational studies. The current study supports the utilization of the leptin resistant Iberian pig for further interventional research in the field.
Antioxidant homeostasis is disturbed in fetuses with leptin-resistant genotypes: a cross-sectional study
(2018) Gonzalez Añover, Pedro; Torres Rovira, Laura; García Contreras, Consolación; Vazquez Gomez, Marta; Pesántez Pacheco, José Luis; Sanz Fernandez, María Victoria; Astiz Balnco, Susana; Gonzalez Bulnes, Antonio
Leptin resistance is associated with lower reproductive efficiency, with deficiencies in embryo viability and growth leading to low prolificacy and high incidence of intrauterine growth restriction.
Differential effects of litter size and within-litter birthweight on postnatal traits of fatty pigs
(2020) Vázquez Gómez, Marta; García Contreras, Consolacion; Pesántez Pacheco, José Luis; Torres Rovira, Laura; Heras Molina, Ana; Astiz, Susana; Óvilo, Cristina; Redondo, Beatriz Isabel; Gonzalez Bulnes, Antonio
The current work evaluated the relative effects of the number of piglets in the litter of origin (high vs. low litter size) and the birthweight (low (LBW) vs. normal (NBW) in large litters) on postnatal performance and quality of carcasses and meat in purebred Iberian pigs. The results indicated that NBW piglets born in large litters had disparities in developmental patterns in weight and size, back-fat deposition, and fatty acid composition of viscerae compared to NBW piglets from small litters, which again were different from those found in LBW piglets when compared to their NBW counterparts. However, both growth patterns were altered and might indicate previous phases of metabolic disorders.
Effects of L-glutamine supplementation during the gestation of gilts and sows on the offspring development in a traditional swine breed
(2021) Vázquez Gómez, Marta; García Contretas, Consolación; Astiz, Susana; Torres Rovira, Laura; Pesántez Pacheco, José Luis; Heras Molina, Ana; Castro Madrigal, Teresa; López Bote, Clemente; Ovilo, Cristina; Gonzalez Bulnes, Antonio; Isabel, Beatriz
The use of amino acids during pregnancy, such as glutamine (Gln), seems to be a promising strategy in selected swine breeds to improve the offspring prenatal development. The main goal of the current study was to assess the development of the offspring from parity 1–3 sows of a traditional breed, which were supplemented with 1% glutamine after Day 35 of gestation, under farm conditions. A total of 486 (288 treated) piglets from 78 (46 treated) Iberian sows were used. At birth and slaughterhouse, fatty acid composition, metabolism, and mTOR pathway gene expression were analyzed. At birth, treated newborns showed greater amounts of specific amino acids in plasma, such as glutamine, asparagine, or alanine, and Σn-3 fatty acids in cellular membranes than control newborns. The expression of genes belonging to mTOR Complex 1 was also higher in treated piglets with normal birth-weight. However, these findings did not improve productive traits at birth or following periods in litters from supplemented gilts (parity 1) or sows (parities 2–3). Thus, further research is needed to properly understand the effects of prenatal glutamine supplementation, particularly in traditional swine breeds.
Effects of maternal factors during pregnancy on the birth weight of lambs in dairy sheep
(Wageningen Academic Publishers, 2018) Pesántez Pacheco, José Luis; Torres Rovira, Laura; Gonzalez Bulnes, Antonio; Astiz, Susana; Vazquez Gomez, Marta; Gonzalez Martin, Juan Vicente; Vazquez Gomez, Marta
The aim of the present study was to evaluate the influence of physiological and productive factors of ewes (age and type of pregnancy and level of milk production) during pregnancy on birth weight of lambs. The study was carried out in a single commercial farm on 334 Lacaune dairy sheep, classified by age (187 mature and 147 maiden ewes), pregnancy type (155 single vs 179 multiple pregnancies), milk production average milk yield per day (YDIM),from conception to drying off; 45 Low yielding, LYDIM, <1.37 l/d; 70 Average yielding, AYDIM, 1.37 to <1.8l/d; 72 High yielding, HYDIM, >1.8 l/d; and average milk yield per day during month of conception (Yc); 45 Low yielding, LYc, <0.91 l/d; 70 Average yielding, AYc, 0.91 to <1.3 l/d; and 72 High yielding, HYc, >1.3 l/d). Lambs body weight was recorded in 576 lambs (253 males, 323 females) at birth and at 18 days old. Differences among groups were evaluated with ANOVA and Kruskal-Wallis test when non-normal distributed. The lambs born to mature ewes were heavier than those from maiden ewes both at birth (4.2±0.8 vs 3.5±0.8 kg; P<0.0001) and at 18 d-old (8.8±2.0 vs 6.8±1.6 kg; P<0.0001). Newborns from single pregnancies were heavier at birth than those born from multiple pregnancies (4.3±0.9 vs 3.8±0.8 kg; P<0.0001), but no difference was found at 18 d-old (8.0±2.1 vs 7.9±2.1 kg; P>0.05). The birth weight of lambs was similar in the groups HYDIM and AYDIM and heavier in these both groups than lambs from the groups LYDIM (4.3±0.8; 4.3±0.8; 4.0±0.8 kg; P<0.05); there were no differences at 18 d-old. Concomitantly, lambs of the groups HYc and AYc were heavier than lambs in the group LYc at birth (4.4±0.8; 4.2±0.7; 4.1±0.8 kg; P>0.05), but values were similar among the three groups at 18 d-old. The present study suggests that, age, type of pregnancy and level of milk production during gestation in ewes are determinants of lamb birth weight.
Effects of maternal factors on the metabolic profile throughout pregnancy in dairy sheep
(WBC 2018 SPONSORS, 2018) Pesántez Pacheco, José Luis; Torres Rovira, Laura; Gonzalez Bulnes, Antonio; Astiz, Susana; Vazquez Gomez, Marta; Gonzalez Martin, Juan Vicente; Gonzalez Martin, Juan Vicente
Objectives: Physiological states as pregnancy and lactation modify metabolism in sheep. The aim of this study was to evaluate the influence of pregnancy features (parity and type of pregnancy) on the maternal metabolism in high yielding Lacaune dairy sheep. Material and methods: The study was carried out in a single commercial farm on 334 Lacaune dairy sheep, classified by age (187 mature and 147 maiden ewes), parity (145 multiparous vs. 42 primiparous) and type of pregnancy (161 single vs. 173 multiple). Fasting plasma samples were collected at two points during pregnancy mid and late-pregnancy (74±5 and 141±3 d, respectively) and postpartum (52±5 d after delivery). The following metabolic parameters were considered: plasma ß-hydroxybutyrate (ß-OHB), nonesterified fatty acids (NEFA), glucose, lactate, urea, cholesterol, triglycerides and fructosamine. Differences among groups and interactions were evaluated with ANOVA and Kruskal-Wallis test when non-normal distributed. Results: ß-OHB was higher during gestation in mature than in maiden ewes (0.58±0.20 vs. 0.37±0.11 mmol/L; P< 0.0001) at mid pregnancy, (0.67±0.34 vs. 0.55±0.21 mmol/L; P< 0.0001) and late pregnancy, but did not differ with age at postpartum (0.78±0.25 vs. 0.74±0.23 mmol/L; P> 0.05). During gestation ß-OHB was not affected by type of pregnancy, although, postpartum ß-OHB was lower in ewes that had carried out single gestation than in those with multiple pregnancy (0.59±0.27 vs. 0.65±0.31 mmol/L; P< 0.01). At mid pregnancy NEFA were lower in mature than in maiden ewes (0.46±0.22 vs. 0.57±0.17 mmol/L; P< 0.0001); whereas NEFA were higher in mature at late pregnancy and postpartum (0.67±0.29 vs. 0.55± 0.18 mmol/L; P< 0.0001 for late pregnancy); (0.50±0.18 vs. 0.44±0.16 mmol/L; P=0.004 for postpartum). At late pregnancy, ewes carrying a single pregnancy showed lower NEFA than those with multiple foetuses (0.57±0.25 vs. 0.67±0.25 mmol/L; P< 0.0001). Glucose levels during gestation did not differ with age, however, at postpartum mature ewes had lower levels than maiden ewes (67.90±6.44 vs. 74.63±7.54 mg/dl P<0.0001). Glucose during gestation was higher in ewes carrying a single gestation at mid (65.45±15.48 vs. 60.44±8.82 mg/ dl; P< 0.004) and late pregnancy (71.84±14.62 vs. 67.37±12.53 mg/dl; P<0.003) but did not differ postpartum. Lactate was lower in mature ewes at mid pregnancy (16.34±8.73 vs. 18.85±10.13 mg/dl; P<0.003), and postpartum (11.13±6.90 vs. 12.07±5.64 mg/dl; P<0.01), but it was similar at late pregnancy and did not change by type of pregnancy. Urea was higher in mature ewes at mid pregnancy (57.44±11.23 vs. 39.95±7.86 mg/dl; P<0.0001) and postpartum (67.56±15.01 vs. 58.18±13.53 mg/dl; P<0.0001), but lower at late pregnancy (41.44±10.70 vs. 42.31±8.81; P<0.048). Urea was higher at late pregnancy in ewes carrying a single gestation (43.29±10.15 vs. 40.45±9.50 mg/dl; P<0.004). Cholesterol was higher during gestation in mature than in maiden ewes (P<0.0001). However, at postpartum cholesterol was higher in maiden ewes (108.79±21.76 vs. 100.44±21.74 mg/dl; P<0.001) and it was not affected by type of pregnancy. Triglycerides were higher in mature ewes (23.51±8.04 vs. 19.29±7.33 mg/dl; P< 0.001) at mid pregnancy. At postpartum, triglycerides were higher in maiden ewes (17.73±8.62 vs. 15.07±4.37mg/dl; P<0.001) and did not differ with type of pregnancy. Fructosamine was higher at mid gestation in mature ewes (310.77±31.63 vs. 275.47±30.58 umol/L; P<0.0001) and at the same time, it was affected by type of pregnancy (289.75±36.95 vs. 300.30±33.90 umol/L for single and multiple pregnancies respectively; P=0.013). The daily average milk yield during the lactation was not different between primiparous and multiparous (312.42±130.68 vs. 339.6±0.142.9 L; P>0.05); before getting pregnant, primiparous produced less than multiparous (1.71±0.62 vs. 2.07±0.67 L; P<0.05), and after conception primiparous produced the same as multiparous (1.11±0.38 vs. 1.07±0.46 L; P>0.05). Milk yield was not affected by type of pregnancy. Conclusions: The present data reveal interactions among maternal factors (age and type of pregnancy) physiological status (pregnancy and lactation) and time of pregnancy on the metabolism of dairy ewes, which be related to health and productivity.
Gestational toxemia in lactating sheep is associated with alterations in circulating inflammatory biomarkers
(WBC 2018 SPONSORS, 2018) Pesántez Pacheco, José Luis; Gonzalez Bulnes, Antonio; Astiz, Susana; Vazquez Gomez, Marta; Gonzalez Martin, Juan Vicente; Sanz Fernandez, María Victoria; Gonzalez Bulnes, Antonio; Astiz, Susana; Gonzalez Martin, Juan Vicente
Objectives: Gestational toxemia (GT) is a late pregnancy metabolic disease characterized by the disruption of glucose and lipid homeostasis. Metabolic dysregulation leads to hepatic failure and neurological disorders, which frequently result in the death of both the ewe and its lamb/s. The etiopathology of GT is poorly understood. Several risk factors have been identified (e.g. age, number of fetuses, nutritional status, etc.); however, the large individual variability in GT susceptibility suggests that other factors are likely involved. Recently, inflammation has been associated with metabolic diseases both in cows and sheep. Thus, the study objective was to evaluate changes in inflammatory biomarkers between toxemic and healthy sheep. Materials and methods: The current dataset was retrospectively obtained as a subset from a larger experiment (n=334) conducted at a Lacaune sheep high-yield dairy farm (“Granja Cerromonte”, Spain). Within individuals treated for GT (clinically diagnosed based on neurological symptoms), sheep with the highest β-hydroxybutyrate (BHB) blood concentrations were selected (n=9; TOX). Matching healthy controls (n=9; CON) were chosen based on lambing date, lactation number (4±3lactations), and number of carried lambs (2±1born lambs). Body condition parameters were recorded and a fasting blood sample (prior to morning feeding) was collected in late gestation (6±2 days before parturition). Results: There were no differences in body weight or body condition score between groups. As expected, TOX sheep had decreased glucose (58.8 vs. 69.4 mg/dl; P < 0.02), and increased non-esterified fatty acids (1.57 vs. 0.72 mM; P < 0.0004; NEFA) and BHB (2.00 vs. 0.84 mM; P < 0.0004) blood concentrations, compared to CON sheep. Circulating cholesterol was decreased in TOX sheep (83.8 vs. 98.4 mg/dl; P < 0.03); but fructosamine, lactate, triglycerides and urea concentrations did not differ between groups. Gestational toxemia increased circulating tumor necrosis factor α (8.4 vs. 5.9 pg/ml; P < 0.002) and decreased haptoglobin (2.4 vs. 7.1 mg/dl; P < 0.03), but did not change interleukin-6 concentrations. Haptoglobin concentrations were negatively correlated with both BHB (r = -0.62; P < 0.006) and NEFA (r = -0.60; P < 0.009) levels. Tumor necrosis factor α concentrations tended to be and were positively correlated with BHB (r = 0.42; P < 0.09) and NEFA (r = 0.82; P < 0.0001) levels. Conclusions: In summary, GT in sheep appears to be associated with alterations in biomarkers of inflammation.
Influence of maternal factors (weight, body condition, parity, and pregnancy rank) on plasma metabolites of dairy ewes and their lambs
(2019) Pesántez Pacheco, José Luis; Heras Molina, Ana; Torres Rovira, Laura; Sanz Fernández, María Victoria; García Contreras, Consolación; Vázquez Gómez, Marta; Feyjoo, Pablo; Cáceres, Elisa; Millán Frías, Mateo; Hernández, Fernando; Martínez Ros, Paula; González Martin, Juan Vicente; Gonzalez Bulnes, Antonio; Astiz, Susana
Pregnancy and lactation are challenging states that affect maternal and lamb health. In Lacaune dairy sheep, we evaluated the impact of parity, pregnancy rank, and body condition on body weight and the condition of ewes and lambs in mid-pregnancy (75 5 d), in late pregnancy (142 4d), and postpartum (52 5d pp). Maternal age was associated with initial decreases, followed by increases, in body weight and condition. After lambing, both mature and maiden ewes lost weight and body condition. Maternal indices of glucose, protein, and lipid metabolism were within physiological values during pregnancy, but postpartum values depended on maternal parity and pregnancy rank, with multiple-pregnant ewes showing a postpartum increase in glucose and maiden sheep a postpartum increase in plasma cholesterol concentration. Male lambs were heavier than female lambs at birth, and lambs born to mothers with higher body condition scores were heavier. Lambs born as singletons were heavier than those born in litters. Maternal age and pregnancy rank did not influence lamb metabolic indicators. Sex affected plasma concentrations of glucose, triglycerides, and cholesterol. Maternal metabolic indicators showed minimal effects on lamb phenotype. These results suggest that, when appropriately fed, dairy sheep can cover the metabolic demands of pregnancy and milk production, regardless of age and pregnancy rank.
Las explotaciones lecheras no tienen que asumir una mala eficiencia reproductiva
(2018) Arias Vaca, Milton Rafael; Torres Rovira, Laura; Hernandez, Fernando; Gonzalez Martin, Juan Vicente; Gonzalez Bulnes, Antonio; Pesántez Pacheco, José Luis
Sin lugar a dudas, la eficiencia reproductiva compromete la rentabilidad de los rebaños. Desde los años 60, hemos ido observando como los indices reproductivos, en especial la tasa de concepción, ha ido disminuyendo a medida que la producción por lactacion y vaca se eleva.
Maternal supplementation with polyphenols and omega-3 fatty acids during pregnancy: effects on growth, metabolism, and body composition of the offspring
(2020) Heras Molina, Ana; Pesántez Pacheco, José Luis; Astiz, Susana; García Contreras, Consolacion; Vázquez Gómez, Marta; Encinas, María Teresa; Óvilo, Cristina; Isabel, Beatriz; Gonzalez Bulnes, Antonio
Maternal supplementation with antioxidants and n3 PUFAs may be a promising strategy to reduce the risk of intrauterine growth restriction and preterm delivery, which may diminish the appearance of low-birth-weight neonates. The present study aimed to determine benefits and risks of a dietary supplementation combining hydroxytyrosol, a polyphenol from olive leaves and fruits, and n3 PUFAs, from linseed oil, on developmental patterns and metabolic traits of offspring in swine, a model of IUGR pregnancies. The results obtained indicate that maternal supplementation with hydroxytyrosol and n-3 fatty acids during pregnancy has no deleterious effects on the reproductive traits of the sows (prolificacy, homogeneity of the litter, and percentage of stillborns and low-birth-weight, LBW, piglets) and the postnatal features of the piglets (growth patterns, adiposity, and metabolic traits). Conversely, in spite of a lower mean weight and corpulence at birth, piglets from the supplemented sows showed higher average daily weight gain and fractional growth rate. Thus, at juvenile stages afterwards, the offspring from the treated group reached higher weight and corpulence, with increased muscle development and better lipidemic and fatty acid profiles, in spite of similar adiposity, than offspring in the control group. However, much caution and more research are still needed before practical recommendation and use in human pregnancies.
Maternal supplementation with polyphenols and omega-3 fatty acids during pregnancy: prenatal effects on growth and metabolism
(2021) Astiz, Susana ; García Contreras, Consolacion; Redondo, Beatriz Isabel; Óvilo, Cristina; Nuñez, Yolanda; Heras Molina, Ana; Pesántez Pacheco, José Luis; Vázquez Gómez, Marta; López García, Adrián; Gonzalez Bulnes, Antonio; Benitez, Rita
Maternal supplementation with antioxidants and n-3 PUFAs may be a promising strategy to reduce the risk of intrauterine growth restriction and preterm delivery, which may diminish the appearance of low-birth-neonates. A previous studies showed beneficial outcomes of the combination of hydroxytyrosol and linoleic acid, but there is no data of its prenatal effects. The present study aimed to determine the possible prenatal implications of such maternal supplementation at prenatal stages in swine, a model of IUGR pregnancies. Results showed effects on litter size, with treated sows having larger litters and, therefore, smaller fetuses. However, the brain/head weight ratio showed a positive effect of the treatment in development, as well as in some other major organs like lungs, spleen, or kidneys. On the other hand, treated piglets showed better glycemic and lipidemic profiles, which could explain postnatal effects. However, further research on the implications of the treatment on litter size and prenatal and postnatal development must be done before practical recommendation can be given.
Metformin alleviates obesity and systemic oxidative stress in obese young swine
(2020) Astiz, Susana; Gonzalez Bulnes, Antonio; Astiz, Isabel; Barbero Fernández, Alicia; Pesántez Pacheco, José Luis; García Contreras, Consolacion; Vázquez Gómez, Marta; Heras Molina, Ana
The present study assessed the relationship between obesity induced by lifestyle and systemic oxidative stress and possible modulations by oral metformin treatments in young individuals, by using a translational swine model of obesity and associated cardiometabolic disorders (Iberian pig). The results indicate the existence of an age-related increase in both adiposity and systemic oxidative stress (using hydrogen peroxide as a marker), which is higher in individuals with obesogenic lifestyle and increased weight and obesity. Such effect was not found in individuals treated with metformin. The translation of these results suggests that childhood obesity increases production of reactive oxygen species (ROS), and therefore systemic oxidative stress. Treatment with metformin would improve such oxidative status.
Observational study on a novel management system involving 10 lambings per year in one high-yield dairy ewe farm
(WBC 2018 SPONSORS, 2018) Pesántez Pacheco, José Luis; Torres Rovira, Laura; Gonzalez Bulnes, Antonio; Astiz, Susana; Vazquez Gomez, Marta; Gonzalez Martin, Juan Vicente; Heras Molina, Ana; Sanz Fernandez, María Victoria; Gonzalez Bulnes, Antonio
Objectives: In order to reduce non-productive days in dairy ewe production, the “accelerated sheep mating systems” can be used. One example is the STAR system, which involves up to 5 lambings/ewe in 3 years (1.2 to 1.4 lambing/ewe/year) through 5 successive annual breeding and lambing seasons (5LY). Despite its feasibility, a major disadvantage to this system is an irregular distribution of the workload throughout the year. For instance, the farm staff capacity is easily overwhelmed during the lambings periods, resulting in a deficient care of the newborns. In an attempt to distribute the workload more evenly, a high-yielding Lacaune farm transitioned from the 5LY system to a novel one consisting on 10 lambing periods per year (10LY). The present study examines the efficiency, productivity and workload concentration in this farm under both systems. Its goal was to determine the effects of a more intensive management system on the farm’s productivity, animal welfare and workload distribution. Material and methods: This observational study was performed in a high-yielding Lacaune dairy ewe farm (“Granja Cerromonte”, Spain). The 5LY system was performed from 2010 to 2012, and the 10LY system from 2014 to 2015. Years 2009 and 2013 were considered transition years. During this period, 27,415 lactations were recorded from an average of 3,746 ewes/year. Results: Daily production (1.70 ± 0.62 l/d vs 1.73 ± 1.66; P = 0.038), interlambing period (302 ± 44 vs 283 ± 50 d; P < 0.0001) and lambings/ewe/year (1.30 ± 0.01 vs1.42 ± 0.01; P < 0.05) slightly worsened in the 10LY system compared to the 5LY one. In contrast, milk yield/lactation (370 ± 156 vs. 349 ± 185 L), lactation length (218±75 vs 192 ± 75 d) and dry period length (53.5 ± 38.3 vs 69.1 ± 34.8 d) (all P < 0.0001) were better in the 10LY system. Most importantly, the workload distribution improved by the 10LY system implementation. During 2010–2012, an average of 889.19 ± 158.47 lambings/lambing period (range, 664 to 1 291) was recorded, resulting in an average of 1,502.73 ± 267.82 live newborn lambs/lambing period and 20.80±18.73 lambings/day (range, 1 to 110). There were 500.6 lambs and 296.6 lambings per worker during lambings periods. Extra work hours were necessary at peak lambings/ day. However, during 2014–2015, an average of 443.66 ± 253.18 lambings/lambing period (range, 85 to 937) was recorded, resulting in 709.85 ± 405.10 live newborn lambs/lambing period and 15.16±7.83 lambings/day (range, 1 to 51). There were 354.5 lambs and 221.5 lambings per worker during lambing periods. Since the lambing and lamb related workload was better distributed in the 10LY-period, the workload/worker fell by 50%, needing only two workers/lambing season to take care of the lambings and lamb pens in the 10LY system (354.5 lambs and 221.5 lambing per worker), compared to the three necessary in the 5LY one (500.6 lambs and 296.6 lambing per worker). Further, culling rate decreased (35.39 ± 0.53 vs 42.51 ± 7.51 % P=0.294) and higher-order lactations increased (24.17 vs 16,08%, P<0.0001) which could be the result of better ewe and lamb management. Another possible benefit could be the increase in the worker’s welfare, since the workload does not concentrate so drastically in certain days. Conclusions: Our study suggests that a 10LY herd management system can be compatible with good profitability and productivity and, more importantly, better animal welfare due to lack of animal overcrowding and better care of lambs and ewes during lambing. Furthermore, it could also mean better working conditions for the farm staff, since the workload is more uniformly distributed throughout the year.
Polyphenols and IUGR pregnancies: effects of maternal hydroxytyrosol supplementation on hepatic fat accretion and energy and fatty acids profile of fetal tissues
(2019) García Contreras, Consolación; Vazquez Gomez, Marta; Pardo, Zaira; Heras Molina, Ana; Pesántez Pacheco, José Luis; Encinas, Teresa; Torres Rovira, Laura; Astiz, Susana; Nieto, Rosa; Ovilo, Cristina; Gonzalez Bulnes, Antonio; Isabel, Beatriz
Maternal supplementation with hydroxytyrosol, a polyphenol present in olive leaves and fruits, is a highly promising strategy to improve the oxidative and metabolic status of fetuses at risk of intrauterine growth restriction, which may diminish the appearance of low-birth-weight neonates. The present study aimed to determine whether hydroxytyrosol, by preventing lipid peroxidation, may influence the fat accretion and energy homeostasis in the liver, as well as the fatty acid composition in the liver and muscle. The results indicate that hydroxytyrosol treatment significantly decreased the energy content of the fetal liver, without a ecting fat accretion, and caused significant changes in the availability of fatty acids. There were significant increases in the amount of total polyunsaturated fatty acids, omega-3 and omega-6, which are highly important for adequate fetal tissue development. However, there were increases in the omega-6/omega-3 ratio and the desaturation index, which make further studies necessary to determine possible e ects on the pro/anti-inflammatory status of the fetuses.
Short-term effects of early menopause on adiposity, fatty acids profile and insulin sensitivity of a swine model of female obesity
(2020) Heras Molina, Ana; Pesántez Pacheco, José Luis; Vázquez Gómez, Marta; García Contretas, Consolación; Astiz, Susana; Redondo Yelamos, Beatriz Isabel; Gonzalez Bulnes, Antonio
Menopause strongly increases incidence and consequences of obesity and non-communicable diseases in women, with recent research suggesting a very early onset of changes in lipid accumulation, dyslipidemia, and insulin resistance. However, there is a lack of adequate preclinical models for its study. The present trial evaluated the usefulness of an alternative method to surgical ovariectomy, the administration of two doses of a GnRH analogue-protein conjugate (Vacsincel®), for inducing ovarian inactivity in sows used as preclinical models of obesity and menopause. All the sows treated with the compound developed ovarian stoppage after the second dose and, when exposed to obesogenic diets during the following three months, showed changes in the patterns of fat deposition, in the fatty acids profiles at the different tissues and in the plasma concentrations of fructosamine, urea, β-hydroxibutirate, and haptoglobin when compared to obese fed with the same diet but maintaining ovarian activity. Altogether, these results indicate that menopause early augments the deleterious effects induced by overfeeding and obesity on metabolic traits, paving the way for future research on physiopathology of these conditions and possible therapeutic targets using the swine model.
Ten years evolution of dairy cattle herds: fertility, production and management
(Scientific Committee, 2018) Pesántez Pacheco, José Luis; Hernandez, Fernando; Gonzalez Bulnes, Antonio; Astiz, Susana; Gonzalez Martin, Juan Vicente; Vazquez Gomez, Marta; Vazquez Gomez, Marta
This study described the productive data evolution from 71 dairy herds over ten years (2008 to 2017). During this period, two global management strategies changed: timed Artificial Insemination (AI) programs since 2011 onwards and composting bedding systems since 2014. The results showed an increase of total cows/ herd (293±299 in 2008 vs 498±558 cattle in 2017), and maximal herd size (980 in 2008 vs 2,505 cattle in 2017). The percentage of heifers (based on the total of animals) was 69.0% without a tendency of change over the years. Regarding production, average daily milk yield per lactating cow increased with time (30.3±2.5 in 2008 vs 33.5±2.2 l/cow in 2017). While calving to first AI interval and estrous detection rate remained stable (91 days and 51.2%, respectively), average days in milk decreased with time (204±14d in 2008 vs 195±16d in 2017). Similarly, the total of AIs per pregnancy decreased from 4.4±1.2 in 2008 to 3.6±1.1 AI/P in 2017. Accordingly to these data, conception and pregnancy rates increased (CR=28.7±5.0% and PR=15.0±3.5% in 2008 vs CR=32.0±5.6% and PR=16.4±4.3 in 2017). Age at first AI decreased from 16.1±1.4 m of age in 2008 to 14.8±51.5 m in 2017, with a reduced age at first calving from 26±1.7 m to 25.1±1.6 m of age in 2017. Neonatal mortality was 8.5%/year during the whole period, with a minimum of 7.2% in 2013 and a maximum of 13.4% in 2008. The annual percentage of culled cows remained stable (29.1±6.8 in 2008 vs 29.8±7.2% in 2017). Dry period length decreased from 64±10 in 2008 to 62±9d in 2017, without an apparent decreasing tendency over the period. In conclusion, the increase in the size of farms is confirmed during this ‘after milk quota period’. The historically impaired fertility with an increasing individual production could not be observed. Moreover, cow’s fertility and heifers reproductive efficiency (age at first calving) improved during this period.