Browsing by Author "Criollo Sumba, Karina Patricia"

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Evaluación de la estabilidad de extractos obtenidos a partir de distintos procesos dE secado de Jungia rugosa
(2016) Criollo Sumba, Karina Patricia; Molina Fernández, Nelly Johanna; León Tamariz, Fabián
The post harvesttreatment applied to medicinal plants get directly involved in their organoleptic and pharmacological characteristics. Within this field, drying methods directly influences on the stability of secondary metabolites, therefore our study was based on the comparison of two methods of drying: stove and lyophilization. By quantifying total phenols, flavonoids and by the evaluation of antioxidant activity through techniques such as DPPH (2, 2 - diphenyl - 1 - picrylhydrazyl) and reducing power, suggesting that the method of drying by lyophilization promotes stability of the polyphenols in comparisson to the method of drying oven. The estimation of the stability of polyphenols was performed by spectrophotometric quantification of metabolites in the dry extracts obtained by comparing their concentrations versus time. Five determinations at times 0, 1, 2, 4 and 6 months were made. Through thin layer chromatography, metabolites present in Jungiarugosawere identifying.,The main present compounds were polyphenols, which showed a higher intensity under fluorescence in plants subjected to freeze-drying. By statistical analysis established that as time goes on there is a decrease in the concentration of total phenols, flavonoids and decrease values AAEAC techniques for reducing power and DPPH. Furthermore, it was also determined that the freeze drying method are obtained higher concentrations than in the oven drying method.