Browsing by Author "Barrera Luna, Geovanny Patricio"

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Evaluación de un conjunto de cumarinas candidatas a inhibidoras de enzimas fosfolipasas A2 (EC 3.1.1.4) de venenos totales de serpientes
(Universidad de Cuenca, 2022-11-18) Barrera Luna, Geovanny Patricio; Cazar Ramírez, María Elena
Phospholipases A2 (PLA2) hydrolyze membrane phospholipids, releasing long-chain fatty acids, but mainly arachidonic acid (ARA), a precursor to the formation of prostaglandins (PG) and leukotrienes (LT) (pro-inflammatory eicosanoids). A possible and interesting anti-inflammatory treatment could be associated with the development of effective anti-inflammatory drugs in selectively inhibiting PLA2, which would prevent the release of AA and, consequently, the formation of PG and LT. Objective: To evaluate, "in vitro", the activity of a set of coumarins that are potentially inhibitory to phospholipase A2 secreted in snake venoms (sPLA2) and, based on these evaluations, to analyze the chemical structure of these molecules to understand their anti-PLA2 enzymatic activity. Methodology: A bibliographic search was carried out for compounds that have reported inhibitory activity against sPLA2 enzymes, either at an experimental and / or theoretical level. Ten coumarin derivatives synthesized by the organic synthesis group, led by Prof. Dr. Eugenio Uriarte Villares and his collaborators, from the University of Santiago de Compostela (USC), were evaluated for their anti-phospholipid activity. The anti-phospholipid activity of ophidian poisons was evaluated by the N-octanoyl benzoic acid (NOBA) method. Results: The structures of the coumarin set evaluated in this study were efficient in inhibiting the phospholipidic activities of sPLA2 in the total venoms of various species of snakes
Hampi. Sistema informático de apoyo a la decisión clínica para el cálculo de dosis terapéutica en pacientes con insuficiencia renal y notificación de reacciones adversas
(2016-11-28) Barrera Luna, Geovanny Patricio; Cárdenas Cordero, Janneth Fernanda; Ochoa Castro, Maritza Raphaela
HAMPI is a computerized clinical decision support system focused on ensuring efficacy and / or avoiding the toxicity of narrow therapeutic drugs, renal elimination and those nephrotoxic agents that may aggravate renal damage; so, that it performs a personalized calculation of the dose for each patient based on their renal function. Renal function is estimated by creatinine clearance, obtained by the modified Cockcroft-Gault formula based on ideal body weight and body surface area; withclearance, the drug dose is calculated through the Dettli nomogram which handles drug variables as Qo and the standard dose for patients with normal renal function, data found in the program database. At the same time, it allows the notification of Adverse Drug Reactions (ADR) through the yellow card form proposed by the Ministry of Public Health of Ecuador (MSP), which was integrated with the Karch and Lasagna algorithm to evaluate the relationship of Causality between medication and ADR. For the validation of the doses calculated by the program, a retrospective study was carried out on patients from the year 2015 diagnosed with CRS at the Vicente Corral Moscoso Hospital, who were prescribed at least one drug susceptible of adjustment, where it was compared the dose calculated by HAMPI with the dose indicated in the international guidelines versus the dose administered in this health home.
Inhibición de enzimas fosfolipasas A2 de venenos totales de serpientes como estrategia en la búsqueda de nuevos fármacos
(2023) Barrera Luna, Geovanny Patricio; Cazar Ramírez, María Elena
Introduction: Nonsteroidal anti-inflammatory drugs (NSAIDs) are widely used for pain therapy, despite its side effects in renal, stomach and coagulant systems. Phospholipases A2 (PLA2) enzymes, present in snakes and bees’ venoms, and even in the human organism, are responsible for several physiological and pathological processes. These enzymes hydrolyze membrane phospholipids, releasing arachidonic acid, a precursor of pro-inflammatory eicosanoids, which give rise to inflammatory mediators. Objective: The aim of the present work is to review new molecules able to block the cleavage of membrane phospholipids by the action of phospholipases A2, preventing the formation of inflammatory mediators. Methodology: A bibliographic revision from literature published from 2011 to 2021 focused on PLA2 inhibitors was carried out. The potential of structure-activity relationship studies is discussed as a strategy to find active compounds against PLA2. Results: 26 studies including natural and synthetic compounds were reviewed and data from 93 molecules with inhibitory activity were collected, highlighting its potential as PLA2 inhibitors. Conclusion: The inhibitory activity of the reviewed compounds could be associated with the substitution patterns in the benzene ring of the molecules. The evaluation of molecular moieties with relevant capacity to inhibit PLA2 will lead to the identification of candidates for synthesis of new enzymes inhibitors.
Medicina tradicional andina
(Universidad de Cuenca, 2020) Brito Roby, Liliana Alexandra; Achig Balarezo, David Ricardo; Angulo Rosero, Aydee Narcisa; Huiracocha Tutivén, María de Lourdes; Orellana Paucar, Adriana Monserrath; Mosquera Vallejo, Lorena Elizabeth; Barrera Luna, Geovanny Patricio
Perceptions towards the practice of andean traditional medicine and the challenges of its integration with modern medicine. Case Cuenca, Ecuador.
(2021) Quinche Guillen, Valeria Estefania; Garzon Lopez, Danilo Esteban; Ansaloni, Raffaella; Barrera Luna, Geovanny Patricio; Orellana Paucar, Adriana Monserrath; Huiracocha Tutivén, María de Lourdes
There is currently a resurgent interest in traditional medicine. The World Health Organization suggested applying strategies for its proper integration into the National Health System. This study seeks to know and understand the opportunities and challenges of the practice of Andean traditional medicine (ATM) in Cuenca (Ecuador) within the context of a possible integration with modern medicine (MM) from the perspective of healers, physicians, and users. The study is qualitative and has a phenomenological design. Convenience and snowball sampling was applied to select participants for focus groups, individual semi-structured interviews, and individual non-participatory observations. The information was qualitatively processed, and the findings categorized into 2 major themes (a. Opportunities in the practice of ATM, and b. Challenges in the potential integration of ATM in MM) and 14 associated subthemes, respectively 6 in the first main theme and 8 in the second main theme. Participants characterized the strengths and weaknesses in integrating Andean traditional medicine with modern medicine. Findings suggest that an appropriate integration of ATM with MM request a government regulatory framework encouraging the protection of ancestral wisdom and biodiversity, a safe and rational application of joint therapies, and research development in the area.
Sabiduría ancestral andina y uso de plantas medicinales
(Universidad de Cuenca, 2020) Brito Roby, Liliana Alexandra