Facultad de Ciencias Químicas-Tesis Maestrías

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Browsing Facultad de Ciencias Químicas-Tesis Maestrías by Author "Curillo Santos, Diana Lorena"

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    Caracterización de la comunidad de hongos endófitos asociados a la raíz de joyapa (Macleania rupestris) y evaluación del efecto de cepas cultivables en el desarrollo de plántulas
    (Universidad de Cuenca, 2022-11-29) Curillo Santos, Diana Lorena; Peña Tapia, Denisse Fabiola
    Macleania rupestris is a native Ericaceae with high potential in terms of benefits for health and the environment. Characterizing generalist or specialized interactions between M. rupestris and associated fungi is key to supporting its conservation and domestication. However, very little is known about the relationship between plant growth promoting endophytes and M. rupestris. Therefore, to learn more about the soil- associated microbiota of M. rupestris, we analyzed endophyte communities associated with the plant's surrounding soil and rhizosphere. Which presented a great abundance of genera such as Proteobacteria and Actinobacteria. rupestris plants. Objective: Characterize the endophytic fungal community associated with the root of joyapa (Macleania rupestris) and evaluate the effect of cultivable strains on seedlings development Methodology: Fungal endophytes were isolated from the roots of M. rupestris to evaluate their growth- promoting activity on seedlings. This research discusses the influence of endophytic fungi on the development of M. A total of six cultivable fungi were extracted, which were identified by the community of isolated fungi and characterized by molecular markers ITS (internal transcribed spacer) and LSU (large subunit). To investigate the effect of endophytes on plant growth, the 6 isolated fungi were selected to evaluate their ability to promote plant growth by applying a strain inoculation bioassay. Results: The results indicated that Clonostachys rosea and Trichoderma paravidescens isolated from the roots of M. rupestris have a vital role in promoting plant growth. Their potential use as inoculants to establish a sustainable crop production system and their application in the discovery of bioactive compounds should be part of future research.