Determinación del poder antibiótico in vitro del extracto etanólico del propóleo sobre Staphylococcus aureus Escherichia coli presentes en metritis puerperal bovina

Abstract

This research work entitle ¨DETERMINATION OF THE ANTIBIOTIC IN VITRO PROPOLIS ETHANOLIC EXTRACT ON Staphylococcus aureus and Escherichia coli THIS IN BOVINE PUERPERAL METRITIS¨, was held in the city of Cuenca, Azuay Province, Republic of Ecuador, whose research execution was in the period between November 2012 and April 2013. This study was aimed to evaluate the in vitro antimicrobial activity of ethanol extracts of Propolis (EEP) as a natural alternative to combat Staphylococcus aureus and Escherichia coli (bacteria isolated in the laboratory under cultivation in sheep blood agar) present in the clinical metritis of dairy cows in the Victoria’s Portete. This action was compared with the sensitivity caused by an antibiotic that offered greater commercial antimicrobial agar plate than Amoxicillin and Clavulanic Acid. They were produced for this ethanolic extracts of Propolis to 10% and 30% Propolis were collected from apiaries in the area and processed in the University of Cuenca. Was used for susceptibility testing of bacteria concentration adjusted to 0.5 McFarland scale and employing Muller Hinton agar. 3 sensitivity discs were used for measuring the effect of Amoxicillin and Clavulanic Acid, EEP sensitivity discs of 10% and 30%, and as a control, ethyl alcohol 96º measures of inhibitory halos generated Propolis treatments whit Amoxicillin and Clavulanic Acid, on Staphylococcus aureus and give no zone of inhibition on Escherichia coli, whereas sensitivity discs ethyl alcohol of 96ºgive no zone of inhibition. The inhibitory action of the halos of the two concentrates are similar Propolis have lower dispersion than the antibiotic employed. This means that the concentrates of Propolis give stable results.