Efecto del flushing energético sobre la población folicular, el número de cuerpos lúteos y calidad ovocitaria en cobayas primerizas

Abstract

The aim of this research was to evaluate the effect of energy flushing administered in primiparous guinea pigs on the follicular population, number of corpora lutea and oocyte quality. Sixty-four primiparous guinea pigs from the Peru line were used and were randomly distributed in 4 dietary treatments with different energy levels, T0 (2400 Kcal/kg of DM), T1 (2600 kcal/kg of DM), T2 (2800 kcal/kg of DM). DM) and T3 (3000 kcal/kg DM). The guinea pigs were fed for 11 days with a forage mixture of red clover plus rye-grass and supplemental feed of corn flour, wheat bran and molasses. All animals were subjected to estrous synchronization by applying PGF2α. At the end of the experiment, the guinea pigs were sacrificed and the ovaries were removed. The COCs were recovered using the slicing method and categorized into three types and histological sections were made to count follicles and corpora lutea. No significant differences were found in the total number of follicles, follicular classification or in the number of corpora lutea. However, the total number of oocytes recovered, the number of category A and B oocytes were (p<0.05) significantly higher in the T3 guinea pigs (222 oocytes), unlike the other treatments. Furthermore, an increase in the percentage of suitable oocytes was observed in T3. In conclusion, increasing the energy level in the diet did not influence the follicular population or the number of corpora lutea, but it did influence the number and quality of oocytes.