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DC Field | Value | Language |
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dc.contributor.author | Santiago Moreno, Julián | |
dc.contributor.author | Tamay Siguenza, Erika Fabiola | |
dc.contributor.author | Palacios Aguilar, Maria Paula | |
dc.contributor.author | Pelaez Zhingri, Gabriela Micaela | |
dc.contributor.author | Saa, Luis Rodrigo | |
dc.contributor.author | Galarza Lucero, Diego Andres | |
dc.contributor.author | Dorado , Jesús | |
dc.date.accessioned | 2023-02-28T18:59:31Z | - |
dc.date.available | 2023-02-28T18:59:31Z | - |
dc.date.issued | 2022 | |
dc.identifier.issn | 1947-5535 | |
dc.identifier.uri | http://dspace.ucuenca.edu.ec/handle/123456789/41161 | - |
dc.identifier.uri | https://www.liebertpub.com/doi/10.1089/bio.2022.0097 | |
dc.description.abstract | This research examined the antioxidant and cryoprotective effects of melatonin (ME) and caffeine (CAF) supplementation in freezing medium on the cryosurvival of Peruvian Paso horse sperm using a two-step accelerating cooling rate. Twenty ejaculates from four adult and fertile stallions were recovered, initially diluted with INRA-96 , and finally frozen with INRA-Freeze with either no supplementation (as control), 1 mM ME, or 2mM CAF using a two-ramp freezing system content inside a cryogenic-box and liquid nitrogen vapors. The sperm kinematic parameters and integrity of the plasma and acrosomal membranes of fresh semen and cryopreserved samples were evaluated using the CASA system (SCA-Evolution 2018) and PI/fluorescein isothiocyanate-conjugated peanut (Arachis hypogaea) agglutinin double fluorescent test, respectively. The oxidative stress of post-thaw sperm samples was also assessed using the CellRox Deep Red fluorescence test. The results showed that curvilinear velocity and average-path velocity were greater ( p < 0.05) after freezing with CAF than the control group. In addition, there were significance differences ( p < 0.01) between stallions (1–4) in post-thaw kinematic parameters regardless of ME or CAF addition. Both ME and CAF improved (p < 0.05) the proportion of sperm with intact plasma membranes and intact acrosomes. Nevertheless, neither CAF nor ME improved the oxidative stress after the cryopreservation process. | |
dc.language.iso | es_ES | |
dc.source | Biopreservation and Biobanking | |
dc.subject | Oxidative stress | |
dc.subject | Melatonin | |
dc.subject | Caffeine | |
dc.subject | Peruvian paso horse | |
dc.subject | Spermatozoa | |
dc.title | Effect of melatonin and caffeine supplementation to freezing medium on cryosurvival of peruvian paso horse sperm using a two-step accelerating cooling rate | |
dc.type | ARTÍCULO | |
dc.ucuenca.idautor | 30536191b | |
dc.ucuenca.idautor | 0105500599 | |
dc.ucuenca.idautor | 1102902291 | |
dc.ucuenca.idautor | 0103912846 | |
dc.ucuenca.idautor | 0104793146 | |
dc.ucuenca.idautor | 0302297833 | |
dc.ucuenca.idautor | 0000-0002-4310-7663 | |
dc.identifier.doi | 10.1089/bio.2022.0097 | |
dc.ucuenca.version | Versión publicada | |
dc.ucuenca.areaconocimientounescoamplio | 08 - Agricultura, Silvicultura, Pesca y Veterinaria | |
dc.ucuenca.afiliacion | Santiago, J., Departamento de Reproducción Animal (INIA), Madrid, España | |
dc.ucuenca.afiliacion | Saa, L., Universidad Técnica Particular de Loja, Loja, Ecuador | |
dc.ucuenca.afiliacion | Galarza, D., Universidad de Cuenca, Facultad de Ciencias Agropecuarias, Cuenca, Ecuador | |
dc.ucuenca.afiliacion | Pelaez, G., Universidad de Cuenca, Facultad de Ciencias Agropecuarias, Cuenca, Ecuador | |
dc.ucuenca.afiliacion | Palacios, M., Universidad de Cuenca, Facultad de Ciencias Agropecuarias, Cuenca, Ecuador | |
dc.ucuenca.afiliacion | Tamay, E., Universidad Técnica Particular de Loja, Loja, Ecuador | |
dc.ucuenca.afiliacion | Dorado, J., Universidad de Córdoba, Córdoba, España | |
dc.ucuenca.correspondencia | Galarza Lucero, Diego Andres, andres.galarza@ucuenca.edu.ec | |
dc.ucuenca.volumen | Volumen 0, número 0 | |
dc.ucuenca.indicebibliografico | SCOPUS | |
dc.ucuenca.factorimpacto | 0.512 | |
dc.ucuenca.cuartil | Q2 | |
dc.ucuenca.numerocitaciones | 0 | |
dc.ucuenca.areaconocimientofrascatiamplio | 4. Ciencias Agrícolas | |
dc.ucuenca.areaconocimientofrascatiespecifico | 4.3 Medicina Veterinaria | |
dc.ucuenca.areaconocimientofrascatidetallado | 4.3.1 Ciencias veterinarias | |
dc.ucuenca.areaconocimientounescoespecifico | 084 - Veterinaria | |
dc.ucuenca.areaconocimientounescodetallado | 0841 - Veterinaria | |
dc.ucuenca.urifuente | https://www.liebertpub.com/ | |
Appears in Collections: | Artículos |
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documento.pdf | 227.75 kB | Adobe PDF | View/Open |
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