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DC Field | Value | Language |
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dc.contributor.author | Chen, Shulin | |
dc.contributor.author | Pelaez Samaniego, Manuel Raul | |
dc.contributor.author | Hiscox, William | |
dc.contributor.author | Bule, Mahesh | |
dc.contributor.author | Wensel, Pierre C. | |
dc.contributor.author | Helms, Gregory L. | |
dc.contributor.author | Davis, William C. | |
dc.contributor.author | Kirchhoff, Helmut | |
dc.contributor.author | Garcia Perez, Manuel | |
dc.date.accessioned | 2022-12-12T14:57:35Z | - |
dc.date.available | 2022-12-12T14:57:35Z | - |
dc.date.issued | 2022 | |
dc.identifier.issn | 2076-3417 | |
dc.identifier.uri | http://dspace.ucuenca.edu.ec/handle/123456789/40452 | - |
dc.identifier.uri | https://api.elsevier.com/content/abstract/scopus_id/85123718385 | |
dc.description.abstract | Overcoming obstacles to commercialization of algal-based processes for biofuels and co-products requires not just piecemeal incremental improvements, but rather a comprehensive and fundamental re-consideration starting with the selected algae and its associated cultivation, harvesting, biomass conversion, and refinement. A novel two-stage process designed to address challenges of mass outdoor microalgal cultivation for biofuels and co-products was previously demonstrated using an oleaginous, haloalkaline-tolerant, and multi-trophic green Chlorella vulgaris. ALP2 from a soda lake. This involved cultivating the microalgae in a fermenter heterotrophically or photobioreactor mixotrophically (first-stage) to rapidly obtain high cell densities and inoculate an open-pond phototrophic culture (second-stage) featuring high levels of NaHCO3, pH, and salinity. An improved two-stage cultivation that instead sustainably used as more cheap and sustainable inputs the organic carbon, nitrogen, and phosphorous from fractionation of waste was here demonstrated in a small-scale biorefinery process. The first cultivation stage consisted of two simultaneous batch flask cultures featuring (1) mixotrophic cell productivity of 7.25 × 107 cells mL−1 day−1 on BG-110 medium supplemented with 1.587 g L−1 urea and an enzymatic hydrolysate of pre-treated (torrefaction + grinding + ozonolysis + soaking ammonia) wheat-straw that corresponded to 10 g L−1 glucose, and (2) mixotrophic cell productivity of 2.25 × 107 cells mL−1 day−1 on BG-110 medium supplemented with 1.587 g L−1 urea and a purified and de-toxified condensate of pre-treated (torrefaction + grinding) wheat straw that corresponded to 0.350 g L−1 of potassium acetate. The second cultivation stage featured 1H NMR-determined phototrophic lipid productivity of 0.045 g triacylglycerides (TAG) L−1 day−1 on BG-110 medium supplemented with 16.8 g L−1 NaHCO3 and fed batch-added 22% (v/v) anaerobically digested food waste effluent at HCl-mediated pH 9.</jats:p> | |
dc.language.iso | es_ES | |
dc.source | Applied Sciences (Switzerland) | |
dc.subject | Anaerobic digestion | |
dc.subject | Algae | |
dc.subject | Biorefinery | |
dc.subject | Enzyme hydrolysis | |
dc.subject | Food and lignocellulosic waste | |
dc.subject | Torrefaction | |
dc.subject | Computer science applications | |
dc.subject | Fluid flow and transfer processes | |
dc.subject | General engineering | |
dc.subject | General materials science | |
dc.subject | Instrumentation | |
dc.subject | Process chemistry and technology | |
dc.title | Biorefinery processing of waste to supply cost-effective and sustainable inputs for two-stage microalgal cultivation | |
dc.type | ARTÍCULO | |
dc.ucuenca.idautor | 0000-0002-3814-0258 | |
dc.ucuenca.idautor | 0000-0002-9353-2123 | |
dc.ucuenca.idautor | 0000-0001-5874-3681 | |
dc.ucuenca.idautor | 0000-0002-9386-2632 | |
dc.ucuenca.idautor | 0301219309 | |
dc.ucuenca.idautor | 0000-0002-3503-1654 | |
dc.ucuenca.idautor | 0000-0003-4173-0416 | |
dc.ucuenca.idautor | 0000-0002-7765-1165 | |
dc.ucuenca.idautor | 0000-0002-0522-0884 | |
dc.identifier.doi | 10.3390/app12031485 | |
dc.ucuenca.version | Versión publicada | |
dc.ucuenca.areaconocimientounescoamplio | 08 - Agricultura, Silvicultura, Pesca y Veterinaria | |
dc.ucuenca.afiliacion | Hiscox, W., University of Washington, Seattle, Estados unidos | |
dc.ucuenca.afiliacion | Kirchhoff, H., University of Washington, Seattle, Estados unidos | |
dc.ucuenca.afiliacion | Davis, W., University of Washington, Seattle, Estados unidos | |
dc.ucuenca.afiliacion | Helms, G., University of Washington, Seattle, Estados unidos | |
dc.ucuenca.afiliacion | Wensel, P., University of Washington, Seattle, Estados unidos | |
dc.ucuenca.afiliacion | Bule, M., University of Washington, Seattle, Estados unidos | |
dc.ucuenca.afiliacion | Pelaez, M., Washington State University, Washington, Estados unidos; Pelaez, M., Universidad de Cuenca, Cuenca, Ecuador | |
dc.ucuenca.afiliacion | Garcia, M., University of Washington, Seattle, Estados unidos | |
dc.ucuenca.afiliacion | Chen, S., University of Washington, Seattle, Estados unidos | |
dc.ucuenca.correspondencia | Wensel, Pierre C., pierrewensel@gmail.com | |
dc.ucuenca.volumen | Volumen 12, número 23 | |
dc.ucuenca.indicebibliografico | SCOPUS | |
dc.ucuenca.factorimpacto | 0.51 | |
dc.ucuenca.cuartil | Q2 | |
dc.ucuenca.numerocitaciones | 0 | |
dc.ucuenca.areaconocimientofrascatiamplio | 1. Ciencias Naturales y Exactas | |
dc.ucuenca.areaconocimientofrascatiespecifico | 1.6 Ciencias Biológicas | |
dc.ucuenca.areaconocimientofrascatidetallado | 1.6.12 Botánica | |
dc.ucuenca.areaconocimientounescoespecifico | 084 - Veterinaria | |
dc.ucuenca.areaconocimientounescodetallado | 0841 - Veterinaria | |
dc.ucuenca.urifuente | https://www.mdpi.com/2076-3417/12/3 | |
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documento.pdf | 5.07 MB | Adobe PDF | View/Open |
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