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DC Field | Value | Language |
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dc.contributor.author | Mendez Alvarez, Maria Silvana | - |
dc.contributor.author | Santiago Moreno, Julián | - |
dc.contributor.author | Galarza Lucero, Diego Andres | - |
dc.contributor.author | Jara Jimbo, Diana Isabel | - |
dc.contributor.author | Paredes Quito, Efraín Bernardo | - |
dc.contributor.author | Muñoz León, Esteban Mauricio | - |
dc.contributor.author | Perea, Fernando P. | - |
dc.contributor.author | Samaniego Campoverde, Jorge Xavier | - |
dc.contributor.author | Soria Parra, Manuel Elias | - |
dc.date.accessioned | 2022-06-10T16:40:55Z | - |
dc.date.available | 2022-06-10T16:40:55Z | - |
dc.date.issued | 2022 | - |
dc.identifier.issn | 0378-4320, e-1873-2232 | - |
dc.identifier.uri | http://dspace.ucuenca.edu.ec/handle/123456789/39094 | - |
dc.identifier.uri | https://www.scopus.com/record/display.uri?eid=2-s2.0-85131145132&origin=resultslist&sort=cp-f&src=s&st1=BoviPure%c2%ae+density-gradient+centrifugation+procedure+enhances+the+quality+of+fresh+and+cryopreserved+dog+epididymal+spermatozoa&sid=8af471bdd14ce7d21da4e32eae2423f6&sot=b&sdt=b&sl=141&s=TITLE-ABS-KEY%28BoviPure%c2%ae+density-gradient+centrifugation+procedure+enhances+the+quality+of+fresh+and+cryopreserved+dog+epididymal+spermatozoa%29&relpos=0&citeCnt=0&searchTerm=&featureToggles=FEATURE_NEW_DOC_DETAILS_EXPORT:1 | - |
dc.description.abstract | BoviPure® is a salt solution containing colloidal silica particles coated with silane used to select sperm (e.g., ruminants) by density-gradient centrifugation (DGC). This research assessed the suitability of the BoviPure-DGC and swim-up methods for selecting dog epididymal sperm in fresh, chilled and frozen-thawed samples on post-treatment sperm quality. Sperm samples (n = 60 epididymides) were recovered by retrograde flushing from thirty orchiectomized adult dogs. Thereafter, 20 sperm pools, containing sperm aliquots of three randomly selected animals, were used for chilling (at 5 ºC for 24 h) and freezing (in liquid nitrogen vapors). Sperm selection by BoviPure-DCG and swim-up was performed in both individual and pooled samples, including non- selected samples as controls. Overall, after BoviPure-DGC selection a higher sperm retrieval rate was obtained than the swim-up selection in both individual (P < 0.05) and pooled (P < 0.01) samples. BoviPure-DGC improved (P < 0.05) the total (TM) and progressive (PSM) sperm mo- tilities, curvilinear (VCL) and straight-line (VSL) velocities, linearity (LIN), wobble (WOB), beat- cross frequency (BCF), and integrity of plasmatic (IPM) and acrosomal (IAM) membranes of in- dividual samples in comparison with non-selected samples. In pooled samples, however, the BoviPure-DGC improved (P < 0.05) the PSM, VCL, WOB, and IPM of chilled and frozen-thawed samples. The swim-up method improved (P < 0.05) only some kinematic variables of the indi- vidual (VCL, WOB and BCF) and cryopreserved pooled samples (VCL and ALH) in comparison with non-selected samples. In conclusion, BoviPure-DGC was more effective for recovering and selecting both fresh and cryopreserved dog epididymal sperm than the swim-up procedure improving the kinematic variables, and membranes intactness | - |
dc.language.iso | es_ES | - |
dc.source | Animal Reproduction Science | - |
dc.subject | BoviPure | - |
dc.subject | Dogs | - |
dc.subject | Sperm selection | - |
dc.subject | Swim-up | - |
dc.subject | Epididymal sperm | - |
dc.title | BoviPure® density-gradient centrifugation procedure enhances the quality of fresh and cryopreserved dog epididymal spermatozoa | - |
dc.type | ARTÍCULO | - |
dc.ucuenca.idautor | 0102423829 | - |
dc.ucuenca.idautor | 0102606373 | - |
dc.ucuenca.idautor | 1801620186 | - |
dc.ucuenca.idautor | 0350007753 | - |
dc.ucuenca.idautor | 0000-0001-8301-6339 | - |
dc.ucuenca.idautor | 0106640402 | - |
dc.ucuenca.idautor | 0105163489 | - |
dc.ucuenca.idautor | 30536191b | - |
dc.ucuenca.idautor | 0103912846 | - |
dc.identifier.doi | 10.1016/j.anireprosci.2022.107003 | - |
dc.ucuenca.version | Versión publicada | - |
dc.ucuenca.areaconocimientounescoamplio | 08 - Agricultura, Silvicultura, Pesca y Veterinaria | - |
dc.ucuenca.afiliacion | Santiago, J., Departamento de Reproducción Animal (INIA), Madrid, España | - |
dc.ucuenca.afiliacion | Paredes, E., Universidad de Cuenca, Facultad de Ciencias Agropecuarias, Cuenca, Ecuador | - |
dc.ucuenca.afiliacion | Galarza, D., Universidad de Cuenca, Facultad de Ciencias Agropecuarias, Cuenca, Ecuador | - |
dc.ucuenca.afiliacion | Samaniego, J., Universidad de Cuenca, Facultad de Ciencias Agropecuarias, Cuenca, Ecuador | - |
dc.ucuenca.afiliacion | Soria, M., Universidad de Cuenca, Facultad de Ciencias Agropecuarias, Cuenca, Ecuador | - |
dc.ucuenca.afiliacion | Mendez, M., Universidad de Cuenca, Facultad de Ciencias Agropecuarias, Cuenca, Ecuador | - |
dc.ucuenca.afiliacion | Muñoz, E., Universidad Católica de Cuenca, Cuenca, Ecuador | - |
dc.ucuenca.afiliacion | Jara, D., Universidad de Cuenca, Facultad de Ciencias Agropecuarias, Cuenca, Ecuador | - |
dc.ucuenca.afiliacion | Perea, F., Laboratorio de Biotecnología de La Reproducción Animal, Facultad de Ciencias Agropecuarias, Universidad de Cuenca, Cuenca, Ecuador | - |
dc.ucuenca.correspondencia | Galarza Lucero, Diego Andres, andres.galarza@ucuenca.edu.ec | - |
dc.ucuenca.volumen | Volumen 242 | - |
dc.ucuenca.indicebibliografico | SCOPUS | - |
dc.ucuenca.factorimpacto | 0.56 | - |
dc.ucuenca.cuartil | Q2 | - |
dc.ucuenca.numerocitaciones | 0 | - |
dc.ucuenca.areaconocimientofrascatiamplio | 4. Ciencias Agrícolas | - |
dc.ucuenca.areaconocimientofrascatiespecifico | 4.3 Medicina Veterinaria | - |
dc.ucuenca.areaconocimientofrascatidetallado | 4.3.1 Ciencias veterinarias | - |
dc.ucuenca.areaconocimientounescoespecifico | 084 - Veterinaria | - |
dc.ucuenca.areaconocimientounescodetallado | 0841 - Veterinaria | - |
dc.ucuenca.urifuente | https://www.sciencedirect.com/journal/animal-reproduction-science/vol/242/suppl/C | - |
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