Efecto de un protocolo hormonal aplicado previo a la aspiración folicular guiada por ultrasonografía sobre la calidad y cantidad de ovocitos en donadoras con alto y bajo recuento de folículos antrales

Abstract

In the experiment two assays were executed, the first one evaluated the appearance of the new follicular wave after a 2 mg dose of estradiol benzoate (EB) plus progesterone implant (CIDR® Active Progesterone 10%, 1,9 g) and 150 µg prostaglandin, in 8 Holstein mixed breed heifers with high and low antral follicle recount (AFC). Every 24h were evaluated by ultrasound the development of the dominant follicle (DF) and the appearance day of the new follicular wave, besides, serum levels of Anti-müllerian Hormone (AMH) were assessed. In the assay two the information of the first one was used, regarding the restart day of the follicular wave, and the protocol effect was assessed on the antral follicles number between 4 and 8 mm observed on the Ovum pick up (OPU) day, as well as, the number and quality of COC’s in donors with high and low AFC. The animals were treatment (with EB) and control (without EB) at a certain time, organizing in treatment T1= High AFC with EB; T2= High AFC without EB; T3=Low AFC with EB and T4= Low AFC without EB. The EB + P4 combination produced the restart of the follicular wave in all heifers at 3, 8 ± 0,25 days. The COC’s recovery percentage of T1 (81, 2 ± 4, 64) was higher compared to T2 (65,1 ± 5,99); T3 (60,3 ± 5,9) and T4 (58,6 ± 10,23). The amount of COC’s suitable for the production of in vitro embryos (PIVE) in T1 was higher compared to T2, T3 y T4 (P<0, 05). It is concluded that treatment T1= High AFC with EB allowed to obtain higher quantity and quality of COC’s recovered in the OPU sessions.