Determinación de la acción antifúngica del propóleo sobre Malassezia spp. en perros domésticos

Abstract

Bees make propolis from initial substances that come from the exudations of plants that involve resins and fluids, exudates from the buds and scales of the leaves of plants and trees, known as propolis. They use it in the construction, repair and protection of the hive. The antifungal properties of propolis have been established by numerous authors, in vitro studies of propolis extracts of different origins show antifungal activity on Dermatophytos and Candida species. This activity depends on the origin of the propolis and the solvent used for its extraction. The objective of this research is to determine the antifungal action of propolis on Malassezia spp. in domestic dogs, by means of the readings of the inhibition halos coming from the antifungigrams. In the canine species (Canis lupus familiaris) the main diseases associated with these microorganisms are external otitis (OE) and dermatitis. Ten samples were taken from domestic dogs clinically diagnosed with external otitis, the samples from the Clínica Solidaria and Veterinaria Diglar, were transported in Stuart medium to the Laboratorio For the effect, 10% EEP sensidisks, 25 μg Fluconazole (FCZ) sensidisks and 96% ethyl alcohol as control were used. We were able to determine that the ethanolic extract of propolis 10% from the Sierra had a maximum inhibition halo of 22mm and it was observed that it was the one that obtained the least dispersion within its group.