Please use this identifier to cite or link to this item:
http://dspace.ucuenca.edu.ec/handle/123456789/37112
Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Rodríguez Coyago, María de Lourdes | - |
dc.contributor.author | Rosa, Alcira Cristina | - |
dc.contributor.author | Nastri, María Lorena | - |
dc.contributor.author | Jewtuchowicz, Virginia Marta | - |
dc.date.accessioned | 2021-10-25T14:11:01Z | - |
dc.date.available | 2021-10-25T14:11:01Z | - |
dc.date.issued | 2021 | - |
dc.identifier.issn | 2613-4950 | - |
dc.identifier.uri | https://www.sciencerepository.org/oral-dysbiosis-exacerbates-candida-parapsilosis-sensu-stricto-biofilm_DOBCR-2021-1-107 | - |
dc.description.abstract | Introduction: Candida parapsilosis sensu stricto is the second to third most frequent cause of candidemia. Studies place this yeast as a frequent colonizer of niches of the oral cavity, predominantly in pathological conditions. We hypothesize that a buccal environment in dysbiosis enhances the virulence of C. parapsilosis sensu stricto. Objective: To evaluate the phenotype and molecular level of the production of biofilm in oral isolates of Candida parapsilosis sensu stricto and correlate the results with the clinical origin (dysbiosis versus eubiosis). Materials and Methods: The biofilm-forming ability was compared in 50 oral isolates of Candida parapsilosis sensu stricto obtained from patients with and without oral dysbiosis; by quantification of metabolic activity. The results were corroborated by confocal fluorescence microscopy, and correlated with the transcriptional activity of CPH2, by RT-qPCR. The data were analysed by Excel 2010, and InfoStat 2018, with a 95% confidence interval. Results: The metabolic activity in biofilm was significantly higher in oral dysbiosis relative to control (p = 0.0025). Basal expression of CPH2 increased 2.8 times more in oral dysbiosis related to the control condition and showed no significant differences with pathogenic isolates of this same yeast, derived from onychomycosis lesions. Conclusion: The oral cavity in dysbiosis increases the virulence of C. parapsilosis sensu stricto due to possible changes in epigenetic marks. This finding suggests that the oral cavity in dysbiosis may be an alternative route to the skin in the epidemiology of nosocomial candidemia. | - |
dc.language.iso | es_ES | - |
dc.source | Dental oral biology and craniofacial research | - |
dc.subject | Candida parapsilosis sensu stricto | - |
dc.subject | Virulence | - |
dc.subject | Oral dysbiosis | - |
dc.subject | Oral eubiosis | - |
dc.subject | CPH2 gene master | - |
dc.title | Oral dysbiosis exacerbates candida parapsilosis sensu stricto biofilm production via up-regulation of the CPH2 biofilm master gene | - |
dc.type | ARTÍCULO | - |
dc.ucuenca.idautor | 0704480276 | - |
dc.ucuenca.idautor | 0000-0002-6516-5303 | - |
dc.ucuenca.idautor | sgrp-3259-004 | - |
dc.ucuenca.idautor | SGRP-3250-3 | - |
dc.identifier.doi | 10.31487/j.DOBCR.2021.01.07 | - |
dc.ucuenca.embargoend | 2050-12-30 | - |
dc.ucuenca.version | Versión publicada | - |
dc.ucuenca.embargointerno | 2050-12-30 | - |
dc.ucuenca.areaconocimientounescoamplio | 09 - Salud y Bienestar | - |
dc.ucuenca.afiliacion | Rodríguez, M., Universidad de Cuenca, Facultad de Odontología, Cuenca, Ecuador; Rodríguez, M., University of Buenos Aires, Buenos Aires, Argentina | - |
dc.ucuenca.afiliacion | Rosa, A., University of Buenos Aires, Buenos Aires, Argentina | - |
dc.ucuenca.afiliacion | Nastri, M., University of Buenos Aires, Buenos Aires, Argentina | - |
dc.ucuenca.afiliacion | Jewtuchowicz, V., University of Buenos Aires, Buenos Aires, Argentina | - |
dc.ucuenca.correspondencia | Rodríguez Coyago, María de Lourdes, maria.rodriguezc84@ucuenca.edu.ec | - |
dc.ucuenca.volumen | Volumen 4, número 1 | - |
dc.ucuenca.indicebibliografico | SIN INDEXAR | - |
dc.ucuenca.numerocitaciones | 0 | - |
dc.ucuenca.areaconocimientofrascatiamplio | 1. Ciencias Naturales y Exactas | - |
dc.ucuenca.areaconocimientofrascatiespecifico | 1.6 Ciencias Biológicas | - |
dc.ucuenca.areaconocimientofrascatidetallado | 1.6.2 Microbiología | - |
dc.ucuenca.areaconocimientounescoespecifico | 091 - Salud | - |
dc.ucuenca.areaconocimientounescodetallado | 0912 - Medicina | - |
dc.ucuenca.urifuente | https://www.sciencerepository.org/dental-oral-biology-and-craniofacial-research | - |
Appears in Collections: | Artículos |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
documento.pdf Until 2050-12-30 | document | 71.64 kB | Adobe PDF | View/Open Request a copy |
This item is protected by original copyright |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.
Centro de Documentacion Regional "Juan Bautista Vázquez" | ||||||||||
|