La Hormona Antimülleriana como un predictor endócrino para estimar la producción de embriones transferibles in vivo

Abstract

The aim of the present study sought to establish whether Antimüllerian hormone (AMH) serves as a reliable marker to estimate endocrine production of transferable embryos in donors Holstein mestiza under grazing systems on 2500 masl. The research was conducted in livestock farms located in the provinces of Azuay and Cañar, Austral area of Ecuador. A total of 36 cows received the same superovulation protocol (SOV). At the beginning of the protocol (day 0), blood samples were taken by coccygeal venipuncture to measure the plasma levels of Antimüllerian hormone, using the DL-AMH-b kit (Develop®). Then the antral follicle count (AFC) was performed for ultrasound, using linear transducer Prosound 2, 7.5 MHz. The assessment of the response to the SOV was performed by counting follicles >8 mm present before the first AI (day 9). On day 15 after embryo collection, the CL number count was performed by ultrasound. Once the collection was completed, the stage and the quality of the structures recovered were assessed based on the criteria of the IETS. The animals were classified into tertiles, having groups of high (0.10 ± 0.002 ng/ml) and low (0.05 ± 0.05 ng/ml) AMH concentration. No correlation was found between the plasma concentration of AMH and the AFC. However, the correlation between AMH and the response to SOV was positive and highly significant (r=0.61; P<0.001). This was also observed with the number of corpus luteum (r= 0.46; P<0.005), the total number of structures recovered (r=0.53; P<0.001) and the number of transferable embryos (r= 0.55; P<0.001). In conclusion, AMH can be used as a reliable endocrine marker for the selection of good embryo donors.