Evaluación in vitro de la actividad antibacteriana de los extractos etanólicos de Uncaria tomentosa; Piper aduncun; Artemisa absinthium frente a Escherichia coli ATCC 25922

Abstract

The plants have been used traditionally for medicinal purposes and could constitute a valuable resource to find new molecules with pharmacological activity in the face of conditions of microbiological origin, especially with the bacterial type. Those that currently constitute a public health problem, mainly due to the resistance acquired from microorganisms to the drugs used in their usual treatment and other factors that contribute to the emergence of resistant bacteria. The purpose of this research was the in-vitro evaluation of the antibacterial activity of ethanol extracts of ethanol plants of Uncaria tomentosa (cat's claw), Piper aduncum (matico), and Artemisa absinthium (wormwood) against the strain of E Coli ATCC 25922. The plant extracts were obtained through the maceration method using 96% ethanol as a solvent. The main functional groups present in the extracts obtained were identified by specific qualitative reactions to confirm the species and subsequently, the antibacterial activity was evaluated by the method of microdilution on the plate standardized by Clinical and Laboratory Standards Institute (CLSI) M100 29 to determine the minimum inhibitory concentration (MIC) of alcoholic extracts against E. coli. The research was exploratory and experimental. It shows the following results: Uncaria tomentosa had a minimum inhibitory concentration (MIC) at 625 µg/ml, while the case of Artemisia absinthium and Piper aduncum no bactericidal effect was observed; however, a slight decrease was observed in bacterial growth so it could arise to hurt that these plants have a bacteriostatic effect.