Evaluación de la eficacia del desinfectante líquido alcalino clorado (ACL 40) frente a coliformes totales y E. coli en las superficies en contacto con alimentos en el área de producción de la empresa New Lac

Abstract

The efficacy of the chlorinated alkaline liquid disinfectant ACL 40 in 5% concentration for 30 minutes of exposure time, was evaluated against total coliforms and E. coli on regular and irregular inert surfaces in contact with food in the production area of the company New Lac. This study was based on a descriptive, observational, cross-sectional research. The sampling was carried out by means of the hyssop method. A total of 240 samples were sampled for later analysis by 3M® Petrifilm Plates: E. coli / Coliforms. In the statistical analysis, the Wilcoxon-Mann-Whitney test was used, because the results were non-parametric. The alternative hypothesis was accepted: "The population of total coliforms and E. coli before the PLD is greater than the population of total coliforms and E. coli after the PLD." However, to show the dispersion of the microbiological counts, histograms were performed and to demonstrate the microbicidal action of the disinfectant, box plots were used, which allowed comparing the bacterial populations of both total coliforms and E. coli before and after the PLD. Finally, the colony count of total coliforms where the significant reduction of the population of total coliforms was observed in most of the regular and irregular inert surfaces, complying with the rule. In the case of E. coli, absence was reported in the microbiological counts, achieving the elimination of the population and thus complying with the regulations, which requires the absence of the microorganism in question.