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Please use this identifier to cite or link to this item: http://dspace.ucuenca.edu.ec/handle/123456789/33121
Title: A modified lateral flow assay, using serum, for the rapid identification of human and bovine cysticercosis in the absence of false positives
Authors: Parkhouse, Michael
Carpio Rodas, Luis Arturo
Campoverde Cisneros, Manuel Alfredo
Sastre, Patricia
Rojas, Glenda C.
Harrison, Leslie
Cortéz, María Milagros
metadata.dc.ucuenca.correspondencia: Parkhouse, Michael, parkhous@igc.gulbenkian.pt
Keywords: Bovine Cysticercosis
Field Diagnosis
Hp10 Antigen Elisa
Hp10 Antigen Lfa
Neurocysticercosis (Ncc)
metadata.dc.ucuenca.areaconocimientofrascatiamplio: 3. Ciencias Médicas y de la Salud
metadata.dc.ucuenca.areaconocimientofrascatidetallado: 3.3.1 Ciencias y Servicios del Cuidado de la Salud
metadata.dc.ucuenca.areaconocimientofrascatiespecifico: 3.3 Ciencias de la Salud
metadata.dc.ucuenca.areaconocimientounescoamplio: 09 - Salud y Bienestar
metadata.dc.ucuenca.areaconocimientounescodetallado: 0912 - Medicina
metadata.dc.ucuenca.areaconocimientounescoespecifico: 091 - Salud
Issue Date: 2019
metadata.dc.ucuenca.embargoend: 29-Jul-2050
metadata.dc.ucuenca.volumen: volumen 113
metadata.dc.source: Transactions of the Royal Society of Tropical Medicine and Hygiene
metadata.dc.identifier.doi: 10.1093/trstmh/try116
metadata.dc.type: ARTÍCULO
Abstract: 
Background Previously we reported the use of a monoclonal antibody–based (HP10) antigen (Ag) detection lateral flow assay (LFA) for the diagnosis of extraparenchymal neurocysticercosis (EP-NCC). The assay performed well when used with cerebrospinal fluid (CSF) samples but not with their paired serum samples, due to false-positive reactions in some known negative control cases. Methods Our novel modification involves pretreatment of serum samples using a combination of sodium deoxycholate and dithiothreitol. Results The modification overcomes the problem of false positives when using negative serum samples from clinically characterized cases of EP-NCC and bovine cysticercosis. In general, there was good agreement between HP10 Ag enzyme-linked immunosorbent assay (ELISA) and the HP10 Ag-LFA, but the HP10 Ag-ELISA was …
Description: 
Background Previously we reported the use of a monoclonal antibody–based (HP10) antigen (Ag) detection lateral flow assay (LFA) for the diagnosis of extraparenchymal neurocysticercosis (EP-NCC). The assay performed well when used with cerebrospinal fluid (CSF) samples but not with their paired serum samples, due to false-positive reactions in some known negative control cases. Methods Our novel modification involves pretreatment of serum samples using a combination of sodium deoxycholate and dithiothreitol. Results The modification overcomes the problem of false positives when using negative serum samples from clinically characterized cases of EP-NCC and bovine cysticercosis. In general, there was good agreement between HP10 Ag enzyme-linked immunosorbent assay (ELISA) and the HP10 Ag-LFA, but the HP10 Ag-ELISA was …
URI: http://dspace.ucuenca.edu.ec/handle/123456789/33121
https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85061121773&origin=inward
metadata.dc.ucuenca.urifuente: http://www.sciencedirect.com/science/journal/00359203
ISSN: 0035-9203
Appears in Collections:Artículos

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