Reacción en cadena de polimerasa transcriptasa inversa para diagnóstico de leucemia mieloide crónica Cuenca 2013-2017

Abstract

Background: The Reverse Transcriptase Polymerase Chain Reaction (RT-PCR), a tool for important diagnosis in the detection of reciprocal translocations between chromosomes, which consists of using ribonucleic acid to generate multiple copies of a specific region through the enzyme Taq polymerase. General objective: General Objective: To determine the prevalence of the BCR / ABL p210 fusion gene by means of the Reverse Transcriptase Polymerase Chain Reaction (RT-PCR) technique in patients with Chronic Myeloid Leukemia (CML). Methodology: Methodology: The design of the research was observational, descriptive and retrospective, carried out from December 2013 to December 2017 and included a total of 121 patients with a diagnosis of CML. The results obtained were analyzed and tabulated with the software SPSS version 23.0 and Excel 2016; they are represented in tables and graphs. Results: The prevalence of the BCR / ABL p210 fusion gene was 38.84% of which positive cases in the city of Cuenca were 24,79 %, male patients accounted for 30,58 % and 9,92 % made up the age group of 28- 37 years. Conclusion: The patients with greater predisposition to the disease were the young adults of the male sex, the city of Cuenca presented the highest number of positive cases and the implementation of the RT-PCR technique was the highly sensitive in vitro diagnostic method for the detection of the gene of fusion BCR / ABL p210